Construct: sgRNA BRDN0001147565
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTAGTGATGCGCGACGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK3 (50808)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76583
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
4741018 |
+ |
AK3 |
NNNAGTGATGCGCGACGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
128945137 |
+ |
GLT1D1 |
NNNAGTGATACGCGACGACA |
NAG |
2 |
0.242 |
Tier II |
| 3 |
NC_000022.11 |
22 |
29125059 |
+ |
KREMEN1 |
NNNAGTGATGCTCGCCGACA |
NGG |
2 |
0.1049 |
Tier II |
| 4 |
NC_000015.10 |
15 |
93944871 |
- |
LINC01581 |
NNNAGTGATTCGCAACGACA |
NGG |
2 |
0.6094 |
Tier III |
| 5 |
NC_000015.10 |
15 |
93944871 |
- |
LINC01580 |
NNNAGTGATTCGCAACGACA |
NGG |
2 |
0.6094 |
Tier III |
| 6 |
NC_000012.12 |
12 |
128945137 |
+ |
AK3P6 |
NNNAGTGATACGCGACGACA |
NAG |
2 |
0.242 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
29047760 |
+ |
Ak3 |
NNNGGTGATGCGTGACGACA |
NGG |
2 |
0.4375 |
Tier I |
| 2 |
NC_000074.6 |
8 |
70319401 |
- |
Gdf1 |
NNNAGTGATGCGTGAGGACA |
NGG |
2 |
0.1077 |
Tier II |
| 3 |
NC_000074.6 |
8 |
70319401 |
- |
Cers1 |
NNNAGTGATGCGTGAGGACA |
NGG |
2 |
0.1077 |
Tier II |
| 4 |
NC_000085.6 |
19 |
29047760 |
+ |
1700018L02Rik |
NNNGGTGATGCGTGACGACA |
NGG |
2 |
0.4375 |
Tier III |
Other clones with same target sequence:
(none)
