Construct: sgRNA BRDN0001147568
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGTAGCCCCATCAAGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK1 (7443)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75522
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
96856140 |
+ |
VRK1 |
NNNGTAGCCCCATCAAGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
17582020 |
- |
NHS |
NNNGTAGCCCCATCAAGAAA |
NGG |
2 |
0.4018 |
Tier II |
| 3 |
NC_000006.12 |
6 |
106506079 |
+ |
CRYBG1 |
NNNGTAGCTCCATCAACACG |
NGG |
2 |
0.2059 |
Tier II |
| 4 |
NC_000003.12 |
3 |
185047164 |
+ |
VPS8 |
NNNGTAGCCACATCAGGACG |
NGG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000015.10 |
15 |
44713818 |
- |
B2M |
NNNCTAGCCCCATCAAGAGG |
NGG |
2 |
0.0662 |
Tier II |
| 6 |
NC_000023.11 |
X |
17582020 |
- |
LOC101928389 |
NNNGTAGCCCCATCAAGAAA |
NGG |
2 |
0.4018 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
106058586 |
+ |
Vrk1 |
NNNGTAGCCCCATCAAGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000072.6 |
6 |
88766670 |
- |
Mgll |
NNNGTGGCCACATCAAGACG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000083.6 |
17 |
5720749 |
+ |
Zdhhc14 |
NNNGAAGCCCCACCAAGACG |
NGG |
2 |
0.3947 |
Tier II |
| 4 |
NC_000069.6 |
3 |
100479753 |
- |
Tent5c |
NNNGTAGCCCCCTCAAGAAG |
NGG |
2 |
0.1128 |
Tier II |
| 5 |
NC_000069.6 |
3 |
8975806 |
+ |
Tpd52 |
NNNGTAGCCCCATGAAGATG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
