Construct: sgRNA BRDN0001147570

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAAGGTGACCTTCTCAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKG1 (5260)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76355

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 56083683 + PHKG1 NNNAGGTGACCTTCTCAGTG NGG 0 1.0 Tier I
2 NC_000014.9 14 20474785 + PNP NNNAGGTGACATTCCCAGTG NGG 2 0.2045 Tier I
3 NC_000017.11 17 63530527 - KCNH6 NNNGGGTGACCTTCTCAGTG NCG 2 0.067 Tier I
4 NC_000007.14 7 56083683 + SUMF2 NNNAGGTGACCTTCTCAGTG NGG 0 1.0 Tier II
5 NC_000010.11 10 98386979 + PYROXD2 NNNTGGTGACCTTCTCAGTG NGG 1 0.6364 Tier II
6 NC_000015.10 15 52132923 + GNB5 NNNAGATCACCTTCTCAGTG NGG 2 0.6154 Tier II
7 NC_000002.12 2 169349262 - LRP2 NNNAGGTCACCCTCTCAGTG NGG 2 0.583 Tier II
8 NC_000019.10 19 48816632 + HSD17B14 NNNAGGTCACCTCCTCAGTG NGG 2 0.4858 Tier II
9 NC_000021.9 21 46530224 - DIP2A NNNAGGTGACCTTCTCACTA NGG 2 0.4464 Tier II
10 NC_000017.11 17 47979338 - CDK5RAP3 NNNAGGTCACCTTCTCAATG NGG 2 0.426 Tier II
11 NC_000014.9 14 64004961 - SYNE2 NNNAGGCCACCTTCTCAGTG NGG 2 0.4231 Tier II
12 NC_000001.11 1 201912915 - LMOD1 NNNTGGTGTCCTTCTCAGTG NGG 2 0.3818 Tier II
13 NC_000001.11 1 113540417 + MAGI3 NNNAGATGACCTTCTTAGTG NGG 2 0.3077 Tier II
14 NC_000017.11 17 17547495 + PEMT NNNAGGTGACCTTCACACTG NGG 2 0.2757 Tier II
15 NC_000019.10 19 56267113 + ZSCAN5A NNNATGTGACCATCTCAGTG NGG 2 0.24 Tier II
16 NC_000011.10 11 102582434 + MMP20 NNNAGGTGACCTTTCCAGTG NGG 2 0.2 Tier II
17 NC_000007.14 7 83381479 + SEMA3E NNNTGGTGACCTTCTTAGTG NGG 2 0.1958 Tier II
18 NC_000012.12 12 2819738 - ITFG2 NNNATGTTACCTTCTCAGTG NGG 2 0.1875 Tier II
19 NC_000001.11 1 150372682 - RPRD2 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
20 NC_000002.12 2 73928465 - DGUOK NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
21 NC_000002.12 2 185826509 + FSIP2 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
22 NC_000003.12 3 54634336 + CACNA2D3 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
23 NC_000004.12 4 75045083 - PARM1 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
24 NC_000006.12 6 31942618 - C2 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
25 NC_000006.12 6 64176059 - EYS NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
26 NC_000006.12 6 100452217 + SIM1 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
27 NC_000007.14 7 103767841 + RELN NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
28 NC_000010.11 10 92017234 - BTAF1 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
29 NC_000017.11 17 31491511 - RAB11FIP4 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
30 NC_000018.10 18 46553807 - LOXHD1 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
31 NC_000018.10 18 69500400 + DOK6 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
32 NC_000019.10 19 38325678 - KCNK6 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
33 NC_000020.11 20 8701439 + PLCB1 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
34 NC_000021.9 21 39665715 + B3GALT5 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier II
35 NC_000005.10 5 142960953 + ARHGAP26 NNNAGGTCACCTTCTCAGAG NGG 2 0.1758 Tier II
36 NC_000005.10 5 147431017 + DPYSL3 NNNCTGTGACCTTCTCAGTG NGG 2 0.1059 Tier II
37 NC_000005.10 5 95598940 + ARSK NNNATGTGACCTTCTCAGAG NGG 2 0.0857 Tier II
38 NC_000023.11 X 124945116 - TENM1 NNNATGTGACCTTCTCAGAG NGG 2 0.0857 Tier II
39 NC_000003.12 3 23936950 + NKIRAS1 NNNAGGTGACTTTCCCAGTG NGG 2 0.0839 Tier II
40 NC_000010.11 10 110020433 - ADD3 NNNAGGTCACCTTCTCTGTG NGG 2 0.0821 Tier II
41 NC_000005.10 5 137442913 + SPOCK1 NNNATGTGACCTGCTCAGTG NGG 2 0.0783 Tier II
42 NC_000007.14 7 688691 + PRKAR1B NNNATGTGACCTTCTCAGTG NAG 2 0.0778 Tier II
43 NC_000011.10 11 11335456 - GALNT18 NNNAGGTGACCTTCTCAGAG NAG 2 0.0741 Tier II
44 NC_000001.11 1 64909984 + JAK1 NNNAGGTGACCTCCTCAGTG NGA 2 0.0548 Tier II
45 NC_000005.10 5 142845583 + ARHGAP26 NNNAGGTGGCCTTCTCAGTG NTG 2 0.026 Tier II
46 NC_000005.10 5 60347784 - PDE4D NNNAGGTGAACTTCTCAGTG NGC 2 0.0193 Tier II
47 NC_000002.12 2 12726131 - TRIB2 NNNAGGTGACCTTCTCAGAG NTG 2 0.0111 Tier II
48 NC_000002.12 2 73093605 - RAB11FIP5 NNNAGGTGACCTGGTCAGTG NGG 2 0.0 Tier II
49 NC_000009.12 9 33380974 + LOC124902142 NNNAAGTCACCTTCTCAGTG NGG 2 0.5333 Tier III
50 NC_000004.12 4 187963703 - LOC124900881 NNNAGGTTACATTCTCAGTG NGG 2 0.4688 Tier III
51 NC_000021.9 21 41686012 + LINC00111 NNNAGGTGACCTCCACAGTG NGG 2 0.4571 Tier III
52 NC_000007.14 7 97338265 + LOC105375416 NNNTGGTTACCTTCTCAGTG NGG 2 0.3977 Tier III
53 NC_000001.11 1 204613241 + LOC105371692 NNNATGTTACCTTCTCAGTG NGG 2 0.1875 Tier III
54 NC_000003.12 3 40446132 - ENTPD3-AS1 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier III
55 NC_000003.12 3 54634336 + ESRG NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier III
56 NC_000004.12 4 75045083 - LOC107986289 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier III
57 NC_000007.14 7 136372792 + LOC105375523 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier III
58 NC_000012.12 12 29872521 - LOC105369715 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier III
59 NC_000020.11 20 31423012 + DEFB122 NNNATGTCACCTTCTCAGTG NGG 2 0.1846 Tier III
60 NC_000001.11 1 166305331 - LOC112268276 NNNAGGTCACCTTCCCAGTG NGG 2 0.1678 Tier III
61 NC_000009.12 9 89677278 - LINC03062 NNNAGGTCACCTTCTCAGGG NGG 2 0.1538 Tier III
62 NC_000010.11 10 46369163 - FAM25BP NNNAGGTGGCCTTCTCGGTG NGG 2 0.1176 Tier III
63 NC_000002.12 2 121934052 + LOC105373592 NNNATGTGACCTTCTCAGGG NGG 2 0.075 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 12664494 - Slc22a1 NNNAGGTGGCCTTCACAGTG NGG 2 0.386 Tier I
2 NC_000071.6 5 129869494 + Phkg1 NNNAGGTGACCTTCTCAGTG NGA 1 0.0694 Tier I
3 NC_000078.6 12 74926291 - Kcnh5 NNNAGGTGATCCTCTCAGTG NGG 2 0.8916 Tier II
4 NC_000070.6 4 132982770 + Fgr NNNAGGTGACCATCTCAGTA NGG 2 0.75 Tier II
5 NC_000080.6 14 99874740 - Klf12 NNNAGGAGACCTTCTCAGTT NGG 2 0.6125 Tier II
6 NC_000071.6 5 19683856 - Magi2 NNNAGGTGCCCATCTCAGTG NGG 2 0.4571 Tier II
7 NC_000084.6 18 38445708 + Ndfip1 NNNAGGTGAGCTTCACAGTG NGG 2 0.2251 Tier II
8 NC_000077.6 11 89416667 + Ankfn1 NNNAGGTGACCTCCTCAGTG NAG 2 0.2047 Tier II
9 NC_000073.6 7 131116175 - Dmbt1 NNNTGGTGACTTTCTCAGTG NGG 2 0.1958 Tier II
10 NC_000068.7 2 4227202 - Frmd4a NNNAGGTGACATTCTCAGGG NGG 2 0.1875 Tier II
11 NC_000076.6 10 11209043 - Shprh NNNATGTTACCTTCTCAGTG NGG 2 0.1875 Tier II
12 NC_000068.7 2 27753408 + Rxra NNNGGGTGACCTGCTCAGTG NGG 2 0.163 Tier II
13 NC_000075.6 9 115921045 - Gadl1 NNNAGGTGACCTCCTCCGTG NGG 2 0.1393 Tier II
14 NC_000086.7 X 133976824 - Sytl4 NNNAAGTGACCTTCTGAGTG NGG 2 0.1333 Tier II
15 NC_000068.7 2 90482667 + Ptprj NNNAGGTGACCCTCTCTGTG NGG 2 0.1263 Tier II
16 NC_000073.6 7 141312715 - Deaf1 NNNAGGTGACCCTCTCTGTG NGG 2 0.1263 Tier II
17 NC_000080.6 14 121360705 - Stk24 NNNAGGTGACTTTCTCAGTG NAG 2 0.0798 Tier II
18 NC_000072.6 6 127915752 - Tspan11 NNNAGGGGACCTTCTCTGTG NGG 2 0.0784 Tier II
19 NC_000078.6 12 110463056 + Ppp2r5c NNNATGTGACCTGCTCAGTG NGG 2 0.0783 Tier II
20 NC_000067.6 1 88519853 + Glrp1 NNNAGGTGACCTTCTTAGTG NGA 2 0.0214 Tier II
21 NC_000074.6 8 18943812 + Xkr5 NNNAGGTGACCTTTTCAGTG NGC 2 0.0163 Tier II
22 NC_000083.6 17 71837042 + Clip4 NNNAGGTGACCTTCGCAGTG NTG 2 0.0019 Tier II
23 NC_000071.6 5 19683856 - Gm52766 NNNAGGTGCCCATCTCAGTG NGG 2 0.4571 Tier III
24 NC_000084.6 18 84750515 + Gm38576 NNNAGGTGAACTTCTCACTG NGG 2 0.4127 Tier III
25 NC_000080.6 14 13283572 - Gm5087 NNNAGGTGACCTGCTCAGTG NTG 2 0.0102 Tier III
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Other clones with same target sequence:

(none)