Construct: sgRNA BRDN0001147570
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAAGGTGACCTTCTCAGTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PHKG1 (5260)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000007.14 | 7 | 56083683 | + | PHKG1 | NNNAGGTGACCTTCTCAGTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000014.9 | 14 | 20474785 | + | PNP | NNNAGGTGACATTCCCAGTG | NGG | 2 | 0.2045 | Tier I |
3 | NC_000017.11 | 17 | 63530527 | - | KCNH6 | NNNGGGTGACCTTCTCAGTG | NCG | 2 | 0.067 | Tier I |
4 | NC_000007.14 | 7 | 56083683 | + | SUMF2 | NNNAGGTGACCTTCTCAGTG | NGG | 0 | 1.0 | Tier II |
5 | NC_000010.11 | 10 | 98386979 | + | PYROXD2 | NNNTGGTGACCTTCTCAGTG | NGG | 1 | 0.6364 | Tier II |
6 | NC_000015.10 | 15 | 52132923 | + | GNB5 | NNNAGATCACCTTCTCAGTG | NGG | 2 | 0.6154 | Tier II |
7 | NC_000002.12 | 2 | 169349262 | - | LRP2 | NNNAGGTCACCCTCTCAGTG | NGG | 2 | 0.583 | Tier II |
8 | NC_000019.10 | 19 | 48816632 | + | HSD17B14 | NNNAGGTCACCTCCTCAGTG | NGG | 2 | 0.4858 | Tier II |
9 | NC_000021.9 | 21 | 46530224 | - | DIP2A | NNNAGGTGACCTTCTCACTA | NGG | 2 | 0.4464 | Tier II |
10 | NC_000017.11 | 17 | 47979338 | - | CDK5RAP3 | NNNAGGTCACCTTCTCAATG | NGG | 2 | 0.426 | Tier II |
11 | NC_000014.9 | 14 | 64004961 | - | SYNE2 | NNNAGGCCACCTTCTCAGTG | NGG | 2 | 0.4231 | Tier II |
12 | NC_000001.11 | 1 | 201912915 | - | LMOD1 | NNNTGGTGTCCTTCTCAGTG | NGG | 2 | 0.3818 | Tier II |
13 | NC_000001.11 | 1 | 113540417 | + | MAGI3 | NNNAGATGACCTTCTTAGTG | NGG | 2 | 0.3077 | Tier II |
14 | NC_000017.11 | 17 | 17547495 | + | PEMT | NNNAGGTGACCTTCACACTG | NGG | 2 | 0.2757 | Tier II |
15 | NC_000019.10 | 19 | 56267113 | + | ZSCAN5A | NNNATGTGACCATCTCAGTG | NGG | 2 | 0.24 | Tier II |
16 | NC_000011.10 | 11 | 102582434 | + | MMP20 | NNNAGGTGACCTTTCCAGTG | NGG | 2 | 0.2 | Tier II |
17 | NC_000007.14 | 7 | 83381479 | + | SEMA3E | NNNTGGTGACCTTCTTAGTG | NGG | 2 | 0.1958 | Tier II |
18 | NC_000012.12 | 12 | 2819738 | - | ITFG2 | NNNATGTTACCTTCTCAGTG | NGG | 2 | 0.1875 | Tier II |
19 | NC_000001.11 | 1 | 150372682 | - | RPRD2 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
20 | NC_000002.12 | 2 | 73928465 | - | DGUOK | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
21 | NC_000002.12 | 2 | 185826509 | + | FSIP2 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
22 | NC_000003.12 | 3 | 54634336 | + | CACNA2D3 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
23 | NC_000004.12 | 4 | 75045083 | - | PARM1 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
24 | NC_000006.12 | 6 | 31942618 | - | C2 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
25 | NC_000006.12 | 6 | 64176059 | - | EYS | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
26 | NC_000006.12 | 6 | 100452217 | + | SIM1 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
27 | NC_000007.14 | 7 | 103767841 | + | RELN | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
28 | NC_000010.11 | 10 | 92017234 | - | BTAF1 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
29 | NC_000017.11 | 17 | 31491511 | - | RAB11FIP4 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
30 | NC_000018.10 | 18 | 46553807 | - | LOXHD1 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
31 | NC_000018.10 | 18 | 69500400 | + | DOK6 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
32 | NC_000019.10 | 19 | 38325678 | - | KCNK6 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
33 | NC_000020.11 | 20 | 8701439 | + | PLCB1 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
34 | NC_000021.9 | 21 | 39665715 | + | B3GALT5 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier II |
35 | NC_000005.10 | 5 | 142960953 | + | ARHGAP26 | NNNAGGTCACCTTCTCAGAG | NGG | 2 | 0.1758 | Tier II |
36 | NC_000005.10 | 5 | 147431017 | + | DPYSL3 | NNNCTGTGACCTTCTCAGTG | NGG | 2 | 0.1059 | Tier II |
37 | NC_000005.10 | 5 | 95598940 | + | ARSK | NNNATGTGACCTTCTCAGAG | NGG | 2 | 0.0857 | Tier II |
38 | NC_000023.11 | X | 124945116 | - | TENM1 | NNNATGTGACCTTCTCAGAG | NGG | 2 | 0.0857 | Tier II |
39 | NC_000003.12 | 3 | 23936950 | + | NKIRAS1 | NNNAGGTGACTTTCCCAGTG | NGG | 2 | 0.0839 | Tier II |
40 | NC_000010.11 | 10 | 110020433 | - | ADD3 | NNNAGGTCACCTTCTCTGTG | NGG | 2 | 0.0821 | Tier II |
41 | NC_000005.10 | 5 | 137442913 | + | SPOCK1 | NNNATGTGACCTGCTCAGTG | NGG | 2 | 0.0783 | Tier II |
42 | NC_000007.14 | 7 | 688691 | + | PRKAR1B | NNNATGTGACCTTCTCAGTG | NAG | 2 | 0.0778 | Tier II |
43 | NC_000011.10 | 11 | 11335456 | - | GALNT18 | NNNAGGTGACCTTCTCAGAG | NAG | 2 | 0.0741 | Tier II |
44 | NC_000001.11 | 1 | 64909984 | + | JAK1 | NNNAGGTGACCTCCTCAGTG | NGA | 2 | 0.0548 | Tier II |
45 | NC_000005.10 | 5 | 142845583 | + | ARHGAP26 | NNNAGGTGGCCTTCTCAGTG | NTG | 2 | 0.026 | Tier II |
46 | NC_000005.10 | 5 | 60347784 | - | PDE4D | NNNAGGTGAACTTCTCAGTG | NGC | 2 | 0.0193 | Tier II |
47 | NC_000002.12 | 2 | 12726131 | - | TRIB2 | NNNAGGTGACCTTCTCAGAG | NTG | 2 | 0.0111 | Tier II |
48 | NC_000002.12 | 2 | 73093605 | - | RAB11FIP5 | NNNAGGTGACCTGGTCAGTG | NGG | 2 | 0.0 | Tier II |
49 | NC_000009.12 | 9 | 33380974 | + | LOC124902142 | NNNAAGTCACCTTCTCAGTG | NGG | 2 | 0.5333 | Tier III |
50 | NC_000004.12 | 4 | 187963703 | - | LOC124900881 | NNNAGGTTACATTCTCAGTG | NGG | 2 | 0.4688 | Tier III |
51 | NC_000021.9 | 21 | 41686012 | + | LINC00111 | NNNAGGTGACCTCCACAGTG | NGG | 2 | 0.4571 | Tier III |
52 | NC_000007.14 | 7 | 97338265 | + | LOC105375416 | NNNTGGTTACCTTCTCAGTG | NGG | 2 | 0.3977 | Tier III |
53 | NC_000001.11 | 1 | 204613241 | + | LOC105371692 | NNNATGTTACCTTCTCAGTG | NGG | 2 | 0.1875 | Tier III |
54 | NC_000003.12 | 3 | 40446132 | - | ENTPD3-AS1 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier III |
55 | NC_000003.12 | 3 | 54634336 | + | ESRG | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier III |
56 | NC_000004.12 | 4 | 75045083 | - | LOC107986289 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier III |
57 | NC_000007.14 | 7 | 136372792 | + | LOC105375523 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier III |
58 | NC_000012.12 | 12 | 29872521 | - | LOC105369715 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier III |
59 | NC_000020.11 | 20 | 31423012 | + | DEFB122 | NNNATGTCACCTTCTCAGTG | NGG | 2 | 0.1846 | Tier III |
60 | NC_000001.11 | 1 | 166305331 | - | LOC112268276 | NNNAGGTCACCTTCCCAGTG | NGG | 2 | 0.1678 | Tier III |
61 | NC_000009.12 | 9 | 89677278 | - | LINC03062 | NNNAGGTCACCTTCTCAGGG | NGG | 2 | 0.1538 | Tier III |
62 | NC_000010.11 | 10 | 46369163 | - | FAM25BP | NNNAGGTGGCCTTCTCGGTG | NGG | 2 | 0.1176 | Tier III |
63 | NC_000002.12 | 2 | 121934052 | + | LOC105373592 | NNNATGTGACCTTCTCAGGG | NGG | 2 | 0.075 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000083.6 | 17 | 12664494 | - | Slc22a1 | NNNAGGTGGCCTTCACAGTG | NGG | 2 | 0.386 | Tier I |
2 | NC_000071.6 | 5 | 129869494 | + | Phkg1 | NNNAGGTGACCTTCTCAGTG | NGA | 1 | 0.0694 | Tier I |
3 | NC_000078.6 | 12 | 74926291 | - | Kcnh5 | NNNAGGTGATCCTCTCAGTG | NGG | 2 | 0.8916 | Tier II |
4 | NC_000070.6 | 4 | 132982770 | + | Fgr | NNNAGGTGACCATCTCAGTA | NGG | 2 | 0.75 | Tier II |
5 | NC_000080.6 | 14 | 99874740 | - | Klf12 | NNNAGGAGACCTTCTCAGTT | NGG | 2 | 0.6125 | Tier II |
6 | NC_000071.6 | 5 | 19683856 | - | Magi2 | NNNAGGTGCCCATCTCAGTG | NGG | 2 | 0.4571 | Tier II |
7 | NC_000084.6 | 18 | 38445708 | + | Ndfip1 | NNNAGGTGAGCTTCACAGTG | NGG | 2 | 0.2251 | Tier II |
8 | NC_000077.6 | 11 | 89416667 | + | Ankfn1 | NNNAGGTGACCTCCTCAGTG | NAG | 2 | 0.2047 | Tier II |
9 | NC_000073.6 | 7 | 131116175 | - | Dmbt1 | NNNTGGTGACTTTCTCAGTG | NGG | 2 | 0.1958 | Tier II |
10 | NC_000068.7 | 2 | 4227202 | - | Frmd4a | NNNAGGTGACATTCTCAGGG | NGG | 2 | 0.1875 | Tier II |
11 | NC_000076.6 | 10 | 11209043 | - | Shprh | NNNATGTTACCTTCTCAGTG | NGG | 2 | 0.1875 | Tier II |
12 | NC_000068.7 | 2 | 27753408 | + | Rxra | NNNGGGTGACCTGCTCAGTG | NGG | 2 | 0.163 | Tier II |
13 | NC_000075.6 | 9 | 115921045 | - | Gadl1 | NNNAGGTGACCTCCTCCGTG | NGG | 2 | 0.1393 | Tier II |
14 | NC_000086.7 | X | 133976824 | - | Sytl4 | NNNAAGTGACCTTCTGAGTG | NGG | 2 | 0.1333 | Tier II |
15 | NC_000068.7 | 2 | 90482667 | + | Ptprj | NNNAGGTGACCCTCTCTGTG | NGG | 2 | 0.1263 | Tier II |
16 | NC_000073.6 | 7 | 141312715 | - | Deaf1 | NNNAGGTGACCCTCTCTGTG | NGG | 2 | 0.1263 | Tier II |
17 | NC_000080.6 | 14 | 121360705 | - | Stk24 | NNNAGGTGACTTTCTCAGTG | NAG | 2 | 0.0798 | Tier II |
18 | NC_000072.6 | 6 | 127915752 | - | Tspan11 | NNNAGGGGACCTTCTCTGTG | NGG | 2 | 0.0784 | Tier II |
19 | NC_000078.6 | 12 | 110463056 | + | Ppp2r5c | NNNATGTGACCTGCTCAGTG | NGG | 2 | 0.0783 | Tier II |
20 | NC_000067.6 | 1 | 88519853 | + | Glrp1 | NNNAGGTGACCTTCTTAGTG | NGA | 2 | 0.0214 | Tier II |
21 | NC_000074.6 | 8 | 18943812 | + | Xkr5 | NNNAGGTGACCTTTTCAGTG | NGC | 2 | 0.0163 | Tier II |
22 | NC_000083.6 | 17 | 71837042 | + | Clip4 | NNNAGGTGACCTTCGCAGTG | NTG | 2 | 0.0019 | Tier II |
23 | NC_000071.6 | 5 | 19683856 | - | Gm52766 | NNNAGGTGCCCATCTCAGTG | NGG | 2 | 0.4571 | Tier III |
24 | NC_000084.6 | 18 | 84750515 | + | Gm38576 | NNNAGGTGAACTTCTCACTG | NGG | 2 | 0.4127 | Tier III |
25 | NC_000080.6 | 14 | 13283572 | - | Gm5087 | NNNAGGTGACCTGCTCAGTG | NTG | 2 | 0.0102 | Tier III |