Construct: sgRNA BRDN0001147571
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCACGTTGTCCGGCACCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTPCR (84284)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76875
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
232955697 |
+ |
NTPCR |
NNNCGTTGTCCGGCACCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
74310046 |
- |
DNAI2 |
NNNCATTGTCCTGCACCCGG |
NGG |
2 |
0.3333 |
Tier I |
| 3 |
NC_000016.10 |
16 |
680832 |
- |
STUB1 |
NNNCGTTCTCCGGCACCCGG |
NGC |
2 |
0.0137 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
114752886 |
- |
Dnaic2 |
NNNCGTTGTCCTGAACCCGG |
NGG |
2 |
0.1346 |
Tier I |
| 2 |
NC_000067.6 |
1 |
10040148 |
- |
Cspp1 |
NNNCGTTGTCAGGCAACCGG |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000085.6 |
19 |
59170408 |
+ |
Vax1 |
NNNGGTTGTCCGACACCCGG |
NGG |
2 |
0.4615 |
Tier II |
Other clones with same target sequence:
(none)
