Construct: sgRNA BRDN0001147576
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATCCACATGGAACAACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UHMK1 (127933)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77184
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
162500073 |
- |
UHMK1 |
NNNTCCACATGGAACAACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
104989416 |
- |
LIN28B |
NNNTCAACATGGCACAACCC |
NGG |
2 |
0.1955 |
Tier II |
| 3 |
NC_000016.10 |
16 |
73687442 |
- |
ZFHX3 |
NNNTCCACATGTAACAACAC |
NGG |
2 |
0.1648 |
Tier II |
| 4 |
NC_000007.14 |
7 |
107446516 |
- |
COG5 |
NNNTTCACATGGACCAACCC |
NGG |
2 |
0.1364 |
Tier II |
| 5 |
NC_000002.12 |
2 |
159108748 |
- |
TANC1 |
NNNTCCACTTGGCACAACCC |
NGG |
2 |
0.1263 |
Tier II |
| 6 |
NC_000020.11 |
20 |
6768804 |
- |
BMP2 |
NNNTCCACATGGAAAAACTC |
NGG |
2 |
0.1026 |
Tier II |
| 7 |
NC_000017.11 |
17 |
77122711 |
- |
SEC14L1 |
NNNTCCTCATGGCACAACCC |
NGG |
2 |
0.0921 |
Tier II |
| 8 |
NC_000017.11 |
17 |
48834209 |
+ |
CALCOCO2 |
NNNTCCTCATGGAACAGCCC |
NGG |
2 |
0.0772 |
Tier II |
| 9 |
NC_000001.11 |
1 |
236542807 |
- |
LGALS8 |
NNNTCCACATGAAATAACCC |
NGG |
2 |
0.0622 |
Tier II |
| 10 |
NC_000012.12 |
12 |
124778904 |
+ |
SCARB1 |
NNNTCAACATGGAACAACCG |
NGG |
2 |
0.0546 |
Tier II |
| 11 |
NC_000023.11 |
X |
44273880 |
- |
EFHC2 |
NNNTCCACATTGAATAACCC |
NGG |
2 |
0.0256 |
Tier II |
| 12 |
NC_000020.11 |
20 |
32478548 |
- |
NOL4L |
NNNTCCACATGGCAGAACCC |
NGG |
2 |
0.0105 |
Tier II |
| 13 |
NC_000002.12 |
2 |
153819594 |
+ |
GALNT13 |
NNNTCCACATGGAACAACAC |
NGT |
2 |
0.0069 |
Tier II |
| 14 |
NC_000020.11 |
20 |
17133225 |
+ |
LOC105372544 |
NNNTCCACATGGATCAAACC |
NGG |
2 |
0.2872 |
Tier III |
| 15 |
NC_000011.10 |
11 |
109816424 |
+ |
LINC02715 |
NNNTCCACATTGAGCAACCC |
NGG |
2 |
0.1795 |
Tier III |
| 16 |
NC_000013.11 |
13 |
49636587 |
- |
LOC105370205 |
NNNTCAACATGGAACAGCCC |
NGG |
2 |
0.1639 |
Tier III |
| 17 |
NC_000003.12 |
3 |
82328059 |
+ |
LINC02008 |
NNNTCCACATGGTATAACCC |
NGG |
2 |
0.02 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
70426154 |
+ |
Gbe1 |
NNNTCCACATGGAGCAACCC |
NGG |
1 |
0.4667 |
Tier II |
| 2 |
NC_000077.6 |
11 |
60164827 |
- |
Rai1 |
NNNTCCCCATGGAACAACCA |
NGG |
2 |
0.2188 |
Tier II |
| 3 |
NC_000084.6 |
18 |
65043992 |
+ |
Nedd4l |
NNNTCCACATAGCACAACCC |
NGG |
2 |
0.2105 |
Tier II |
| 4 |
NC_000078.6 |
12 |
105728034 |
- |
Ak7 |
NNNTCAACATGGAACACCCC |
NGG |
2 |
0.1639 |
Tier II |
| 5 |
NC_000077.6 |
11 |
72127969 |
+ |
Pitpnm3 |
NNNTCCACATGGAACAGCAC |
NGG |
2 |
0.0756 |
Tier II |
| 6 |
NC_000084.6 |
18 |
76083761 |
+ |
Zbtb7c |
NNNTCCACAAGGAATAACCC |
NGG |
2 |
0.0571 |
Tier II |
| 7 |
NC_000081.6 |
15 |
100329339 |
+ |
Mettl7a1 |
NNNTCCACATGGAATAAACC |
NGG |
2 |
0.0359 |
Tier II |
| 8 |
NC_000075.6 |
9 |
97717533 |
+ |
Clstn2 |
NNNTCCACAGGGAATAACCC |
NGG |
2 |
0.0333 |
Tier II |
| 9 |
NC_000086.7 |
X |
95881108 |
+ |
Zc3h12b |
NNNTCCACATGGAAAAAGCC |
NGG |
2 |
0.0296 |
Tier II |
| 10 |
NC_000068.7 |
2 |
6576362 |
+ |
Celf2 |
NNNTACACATGGAACAACCC |
NTG |
2 |
0.0223 |
Tier II |
| 11 |
NC_000078.6 |
12 |
29694015 |
+ |
Myt1l |
NNNTACACATGGAACAACCC |
NTG |
2 |
0.0223 |
Tier II |
| 12 |
NC_000082.6 |
16 |
30265145 |
- |
Cpn2 |
NNNTCCACATGGAACAATCC |
NGT |
2 |
0.0104 |
Tier II |
| 13 |
NC_000079.6 |
13 |
37363033 |
+ |
Ly86 |
NNNTCCACATGGAAAAACCC |
NGC |
2 |
0.0049 |
Tier II |
| 14 |
NC_000071.6 |
5 |
76828481 |
+ |
Cracd |
NNNTCCACATGGAACAACCG |
NGA |
2 |
0.0041 |
Tier II |
| 15 |
NC_000070.6 |
4 |
109684087 |
- |
Faf1 |
NNNTCCACATGGAAAAACCC |
NGT |
2 |
0.0036 |
Tier II |
| 16 |
NC_000084.6 |
18 |
65043992 |
+ |
Gm41756 |
NNNTCCACATAGCACAACCC |
NGG |
2 |
0.2105 |
Tier III |
| 17 |
NC_000071.6 |
5 |
119685312 |
+ |
Tbx3os2 |
NNNTCCACATGGAACACCCA |
NGG |
2 |
0.0882 |
Tier III |
| 18 |
NC_000071.6 |
5 |
76828481 |
+ |
Gm52818 |
NNNTCCACATGGAACAACCG |
NGA |
2 |
0.0041 |
Tier III |
Other clones with same target sequence:
(none)
