Construct: sgRNA BRDN0001147576

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCATCCACATGGAACAACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UHMK1 (127933)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77184

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 162500073 - UHMK1 NNNTCCACATGGAACAACCC NGG 0 1.0 Tier I
2 NC_000006.12 6 104989416 - LIN28B NNNTCAACATGGCACAACCC NGG 2 0.1955 Tier II
3 NC_000016.10 16 73687442 - ZFHX3 NNNTCCACATGTAACAACAC NGG 2 0.1648 Tier II
4 NC_000007.14 7 107446516 - COG5 NNNTTCACATGGACCAACCC NGG 2 0.1364 Tier II
5 NC_000002.12 2 159108748 - TANC1 NNNTCCACTTGGCACAACCC NGG 2 0.1263 Tier II
6 NC_000020.11 20 6768804 - BMP2 NNNTCCACATGGAAAAACTC NGG 2 0.1026 Tier II
7 NC_000017.11 17 77122711 - SEC14L1 NNNTCCTCATGGCACAACCC NGG 2 0.0921 Tier II
8 NC_000017.11 17 48834209 + CALCOCO2 NNNTCCTCATGGAACAGCCC NGG 2 0.0772 Tier II
9 NC_000001.11 1 236542807 - LGALS8 NNNTCCACATGAAATAACCC NGG 2 0.0622 Tier II
10 NC_000012.12 12 124778904 + SCARB1 NNNTCAACATGGAACAACCG NGG 2 0.0546 Tier II
11 NC_000023.11 X 44273880 - EFHC2 NNNTCCACATTGAATAACCC NGG 2 0.0256 Tier II
12 NC_000020.11 20 32478548 - NOL4L NNNTCCACATGGCAGAACCC NGG 2 0.0105 Tier II
13 NC_000002.12 2 153819594 + GALNT13 NNNTCCACATGGAACAACAC NGT 2 0.0069 Tier II
14 NC_000020.11 20 17133225 + LOC105372544 NNNTCCACATGGATCAAACC NGG 2 0.2872 Tier III
15 NC_000011.10 11 109816424 + LINC02715 NNNTCCACATTGAGCAACCC NGG 2 0.1795 Tier III
16 NC_000013.11 13 49636587 - LOC105370205 NNNTCAACATGGAACAGCCC NGG 2 0.1639 Tier III
17 NC_000003.12 3 82328059 + LINC02008 NNNTCCACATGGTATAACCC NGG 2 0.02 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 70426154 + Gbe1 NNNTCCACATGGAGCAACCC NGG 1 0.4667 Tier II
2 NC_000077.6 11 60164827 - Rai1 NNNTCCCCATGGAACAACCA NGG 2 0.2188 Tier II
3 NC_000084.6 18 65043992 + Nedd4l NNNTCCACATAGCACAACCC NGG 2 0.2105 Tier II
4 NC_000078.6 12 105728034 - Ak7 NNNTCAACATGGAACACCCC NGG 2 0.1639 Tier II
5 NC_000077.6 11 72127969 + Pitpnm3 NNNTCCACATGGAACAGCAC NGG 2 0.0756 Tier II
6 NC_000084.6 18 76083761 + Zbtb7c NNNTCCACAAGGAATAACCC NGG 2 0.0571 Tier II
7 NC_000081.6 15 100329339 + Mettl7a1 NNNTCCACATGGAATAAACC NGG 2 0.0359 Tier II
8 NC_000075.6 9 97717533 + Clstn2 NNNTCCACAGGGAATAACCC NGG 2 0.0333 Tier II
9 NC_000086.7 X 95881108 + Zc3h12b NNNTCCACATGGAAAAAGCC NGG 2 0.0296 Tier II
10 NC_000068.7 2 6576362 + Celf2 NNNTACACATGGAACAACCC NTG 2 0.0223 Tier II
11 NC_000078.6 12 29694015 + Myt1l NNNTACACATGGAACAACCC NTG 2 0.0223 Tier II
12 NC_000082.6 16 30265145 - Cpn2 NNNTCCACATGGAACAATCC NGT 2 0.0104 Tier II
13 NC_000079.6 13 37363033 + Ly86 NNNTCCACATGGAAAAACCC NGC 2 0.0049 Tier II
14 NC_000071.6 5 76828481 + Cracd NNNTCCACATGGAACAACCG NGA 2 0.0041 Tier II
15 NC_000070.6 4 109684087 - Faf1 NNNTCCACATGGAAAAACCC NGT 2 0.0036 Tier II
16 NC_000084.6 18 65043992 + Gm41756 NNNTCCACATAGCACAACCC NGG 2 0.2105 Tier III
17 NC_000071.6 5 119685312 + Tbx3os2 NNNTCCACATGGAACACCCA NGG 2 0.0882 Tier III
18 NC_000071.6 5 76828481 + Gm52818 NNNTCCACATGGAACAACCG NGA 2 0.0041 Tier III
Download CSV

Other clones with same target sequence:

(none)

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