Construct: sgRNA BRDN0001147582
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTAGGCTATAATCACAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABL1 (25)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77732
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
130854834 |
+ |
ABL1 |
NNNAGGCTATAATCACAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
162457585 |
+ |
PRKN |
NNNAAGCTAAAATCACAATG |
NGG |
2 |
0.7429 |
Tier II |
| 3 |
NC_000013.11 |
13 |
101887030 |
+ |
FGF14 |
NNNTGGCTATAATCAAAATG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000011.10 |
11 |
4991344 |
- |
MMP26 |
NNNAAGCTATGATCACAATG |
NGG |
2 |
0.5633 |
Tier II |
| 5 |
NC_000010.11 |
10 |
6306489 |
+ |
PFKFB3 |
NNNAGGCCATCATCACAATG |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000009.12 |
9 |
69000052 |
+ |
PIP5K1B |
NNNAGGCTACAATTACAATG |
NGG |
2 |
0.3911 |
Tier II |
| 7 |
NC_000018.10 |
18 |
6393761 |
- |
L3MBTL4 |
NNNAGGCTATGATCACAATC |
NGG |
2 |
0.2786 |
Tier II |
| 8 |
NC_000013.11 |
13 |
46007561 |
+ |
ZC3H13 |
NNNTGGCTATAATCACAATC |
NGG |
2 |
0.2727 |
Tier II |
| 9 |
NC_000007.14 |
7 |
103848716 |
+ |
RELN |
NNNAGACTATACTCACAATG |
NGG |
2 |
0.2632 |
Tier II |
| 10 |
NC_000003.12 |
3 |
186281064 |
- |
DGKG |
NNNAAGCTATAATCACAAAG |
NGG |
2 |
0.2476 |
Tier II |
| 11 |
NC_000015.10 |
15 |
80697243 |
- |
ABHD17C |
NNNAAGCTATAATCCCAATG |
NGG |
2 |
0.2364 |
Tier II |
| 12 |
NC_000007.14 |
7 |
39086643 |
- |
POU6F2 |
NNNATGCTATAAACACAATG |
NGG |
2 |
0.2077 |
Tier II |
| 13 |
NC_000015.10 |
15 |
80493595 |
- |
ARNT2 |
NNNAGGGTATAATCACAGTG |
NGG |
2 |
0.1882 |
Tier II |
| 14 |
NC_000011.10 |
11 |
132854487 |
+ |
OPCML |
NNNAGGCTCTAATCACAAAG |
NGG |
2 |
0.1633 |
Tier II |
| 15 |
NC_000002.12 |
2 |
165557000 |
- |
CSRNP3 |
NNNAGCCTATAATCACACTG |
NGG |
2 |
0.1299 |
Tier II |
| 16 |
NC_000003.12 |
3 |
51827232 |
+ |
IQCF3 |
NNNAGGATATAATCACAATG |
NTG |
2 |
0.0292 |
Tier II |
| 17 |
NC_000015.10 |
15 |
80493595 |
- |
LOC124903537 |
NNNAGGGTATAATCACAGTG |
NGG |
2 |
0.1882 |
Tier III |
| 18 |
NC_000004.12 |
4 |
78669063 |
- |
LINC01094 |
NNNAGGCTATAAGCTCAATG |
NGG |
2 |
0.0522 |
Tier III |
| 19 |
NC_000003.12 |
3 |
168632745 |
- |
EGFEM1P |
NNNAGGATATAATCACAATG |
NGC |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
31778954 |
+ |
Abl1 |
NNNGGGTTATAATCACAATG |
NGG |
2 |
0.5078 |
Tier I |
| 2 |
NC_000082.6 |
16 |
90236971 |
+ |
Scaf4 |
NNNAGACTATAATCAAAATG |
NGG |
2 |
1.0 |
Tier II |
| 3 |
NC_000078.6 |
12 |
35930579 |
- |
Agr3 |
NNNAGGTCATAATCACAATG |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000067.6 |
1 |
178831817 |
- |
Kif26b |
NNNTGGCTACAATCACAATG |
NGG |
2 |
0.3394 |
Tier II |
| 5 |
NC_000070.6 |
4 |
43731429 |
+ |
Spaar |
NNNAGGCTCTACTCACAATG |
NGG |
2 |
0.1504 |
Tier II |
| 6 |
NC_000071.6 |
5 |
96664791 |
+ |
Fras1 |
NNNAGGCTATAATTAGAATG |
NGG |
2 |
0.1128 |
Tier II |
| 7 |
NC_000075.6 |
9 |
66123539 |
- |
Snx1 |
NNNAGGCTATAATCATAAAG |
NGG |
2 |
0.0879 |
Tier II |
| 8 |
NC_000086.7 |
X |
68778916 |
- |
Fmr1nb |
NNNAGACTATAATCACAATG |
NGA |
2 |
0.0694 |
Tier II |
| 9 |
NC_000074.6 |
8 |
19731488 |
- |
4930467E23Rik |
NNNAGGCTATAAGCACAATG |
NAG |
2 |
0.0676 |
Tier II |
| 10 |
NC_000074.6 |
8 |
20361291 |
+ |
Gm15319 |
NNNAGGCTATAAGCACAATG |
NAG |
2 |
0.0676 |
Tier II |
| 11 |
NC_000074.6 |
8 |
20616839 |
- |
Gm21119 |
NNNAGGCTATAAGCACAATG |
NAG |
2 |
0.0676 |
Tier II |
| 12 |
NC_000071.6 |
5 |
13395585 |
+ |
Sema3a |
NNNATGCTATAATCACAATG |
NGA |
2 |
0.0208 |
Tier II |
| 13 |
NC_000067.6 |
1 |
102213536 |
- |
Gm3116 |
NNNAGACTATAATCACCATG |
NGG |
2 |
0.1765 |
Tier III |
| 14 |
NC_000070.6 |
4 |
94266985 |
+ |
Gm12648 |
NNNTGGCTATAATCACAATG |
NAG |
2 |
0.165 |
Tier III |
| 15 |
NC_000073.6 |
7 |
105841729 |
- |
9130208D14Rik |
NNNAGGCTATAATCACAGTG |
NAG |
2 |
0.1037 |
Tier III |
| 16 |
NC_000074.6 |
8 |
20829927 |
- |
Gm20946 |
NNNAGGCTATAAGCACAATG |
NAG |
2 |
0.0676 |
Tier III |
| 17 |
NC_000074.6 |
8 |
25366244 |
+ |
Gm39146 |
NNNAGGCTATAATCAGAGTG |
NGG |
2 |
0.0615 |
Tier III |
Other clones with same target sequence:
(none)
