Construct: sgRNA BRDN0001147584

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGCTGTACAGGGTGTACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLK (51701)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75482

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 28043055 - NLK NNNCTGTACAGGGTGTACAG NGG 0 1.0 Tier I
2 NC_000013.11 13 98498728 - STK24 NNNTTGTGCAGGGTGTACAG NGG 2 0.5867 Tier II
3 NC_000020.11 20 34082089 + RALY NNNCTGAAGAGGGTGTACAG NGG 2 0.5417 Tier II
4 NC_000001.11 1 120017258 - NOTCH2 NNNCTGAACAGCGTGTACAG NGG 2 0.4632 Tier II
5 NC_000001.11 1 120776368 + NBPF26 NNNCTGAACAGCGTGTACAG NGG 2 0.4632 Tier II
6 NC_000001.11 1 120776368 + NOTCH2NLR NNNCTGAACAGCGTGTACAG NGG 2 0.4632 Tier II
7 NC_000001.11 1 146176673 - NOTCH2NLA NNNCTGAACAGCGTGTACAG NGG 2 0.4632 Tier II
8 NC_000001.11 1 148627608 - NOTCH2NLB NNNCTGAACAGCGTGTACAG NGG 2 0.4632 Tier II
9 NC_000001.11 1 149443660 + NOTCH2NLC NNNCTGAACAGCGTGTACAG NGG 2 0.4632 Tier II
10 NC_000009.12 9 133110264 - RALGDS NNNCTGCACAGGGTGCACAG NGG 2 0.4583 Tier II
11 NC_000013.11 13 59713152 - DIAPH3 NNNCTGTACTTGGTGTACAG NGG 2 0.3394 Tier II
12 NC_000015.10 15 34393215 - GOLGA8A NNNCAGTACAGGGTGTATAG NGG 2 0.3214 Tier II
13 NC_000015.10 15 34539436 - GOLGA8B NNNCAGTACAGGGTGTATAG NGG 2 0.3214 Tier II
14 NC_000010.11 10 119426340 - GRK5 NNNCTGTGCAGGGTGGACAG NGG 2 0.2538 Tier II
15 NC_000017.11 17 29479089 - TAOK1 NNNGTGTACAGTGTGTACAG NGG 2 0.1923 Tier II
16 NC_000017.11 17 17045554 + MPRIP NNNCTGTTCAGGGTCTACAG NGG 2 0.1169 Tier II
17 NC_000018.10 18 14917658 - ANKRD30B NNNCTGTTCAGGGTGTACAG NAG 2 0.1111 Tier II
18 NC_000008.11 8 47837493 + PRKDC NNNCTGTACAGGGTGTCCTG NGG 2 0.095 Tier II
19 NC_000008.11 8 129933492 + CYRIB NNNCTGGACAGGGTTTACAG NGG 2 0.084 Tier II
20 NC_000020.11 20 32293797 - KIF3B NNNCTGTACAGAGTGTACAG NGA 2 0.0648 Tier II
21 NC_000012.12 12 44089008 - TMEM117 NNNATGTACAGGGTGTACAG NTG 2 0.0328 Tier II
22 NC_000008.11 8 35442033 - UNC5D NNNCTGTACAGGTTGTACAG NGT 2 0.0048 Tier II
23 NC_000010.11 10 1544612 + ADARB2 NNNCTGGACAGGGGGTACAG NGG 2 0.0 Tier II
24 NC_000013.11 13 87431942 + LOC105370302 NNNCTGTAGAGGGTGTACAA NGG 2 0.5804 Tier III
25 NC_000005.10 5 95627850 + LOC112267933 NNNCTGTACAGGGAGCACAG NGG 2 0.4127 Tier III
26 NC_000002.12 2 34084081 + LINC01317 NNNCTGTACAGTGTGTACAG NAG 2 0.0997 Tier III
27 NC_000010.11 10 1544612 + ADARB2-AS1 NNNCTGGACAGGGGGTACAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 125630774 + Il21r NNNCTGTACAGGGAGCACAG NGG 2 0.4127 Tier I
2 NC_000070.6 4 121500431 - Gm12880 NNNCTGAACAGAGTGTACAG NGG 2 0.8167 Tier II
3 NC_000077.6 11 115585657 + Gga3 NNNCTGTTCAGGGTGTACAG NGG 1 0.4286 Tier II
4 NC_000068.7 2 165027367 - Ncoa5 NNNCTGTACAGGCTGTACAG NGG 1 0.4211 Tier II
5 NC_000074.6 8 75910021 - Iqcm NNNCTGTCCAGAGTGTACAG NGG 2 0.4 Tier II
6 NC_000070.6 4 140053359 + Igsf21 NNNCTGTTCTGGGTGTACAG NGG 2 0.3782 Tier II
7 NC_000079.6 13 24599380 - Ripor2 NNNCTGTACTGGGTGTACAG NAG 2 0.2288 Tier II
8 NC_000070.6 4 137552007 - Hspg2 NNNATGTACAGGGTGTGCAG NGG 2 0.1486 Tier II
9 NC_000068.7 2 181264061 + Gmeb2 NNNCTGTTCAGGGTGGACAG NGG 2 0.1484 Tier II
10 NC_000072.6 6 33769167 + Exoc4 NNNCTGTACAGGGTGAAGAG NGG 2 0.1212 Tier II
11 NC_000069.6 3 9775147 + Pag1 NNNCTGTGCAGGGTTTACAG NGG 2 0.1048 Tier II
12 NC_000085.6 19 50226872 + Sorcs1 NNNCTCTACAGGGTTTACAG NGG 2 0.0974 Tier II
13 NC_000075.6 9 71749618 - Cgnl1 NNNCTGTACAGGGTGTAGAG NAG 2 0.0346 Tier II
14 NC_000071.6 5 100165575 - Tmem150c NNNCTGTACAAGGTGTACAG NGT 2 0.0161 Tier II
15 NC_000073.6 7 46212868 - Ush1c NNNCTGTACAGGGGGTACAG NTG 2 0.0 Tier II
16 NC_000068.7 2 5034760 + Optn NNNCTGTACAGGGGTTACAG NGG 2 0.0 Tier II
17 NC_000084.6 18 83484674 + Gm31565 NNNGTGTACAGAGTGTACAG NGG 2 0.4667 Tier III
18 NC_000079.6 13 24599380 - Gm11346 NNNCTGTACTGGGTGTACAG NAG 2 0.2288 Tier III
19 NC_000080.6 14 79690698 + Gm41218 NNNCTGTTCAGGGTGTACAG NTG 2 0.0167 Tier III
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Other clones with same target sequence:

(none)