Construct: sgRNA BRDN0001147587

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGCTTTGATTTATATGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATR (545)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77551

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 142559256 - ATR NNNGCTTTGATTTATATGCA NGG 0 1.0 Tier I
2 NC_000022.11 22 36227543 + APOL2 NNNGCTTTGACTCATATGCA NGG 2 0.5263 Tier I
3 NC_000001.11 1 175645915 - TNR NNNGCTTTGTCTTATATGCA NGG 2 0.5882 Tier II
4 NC_000016.10 16 61884196 + CDH8 NNNGATTTGATTTATATGCA NGG 1 0.5714 Tier II
5 NC_000005.10 5 37161180 - CPLANE1 NNNACTTTGATTTATATGTA NGG 2 0.4154 Tier II
6 NC_000008.11 8 75492819 + HNF4G NNNGCTTTGAATTATAAGCA NGG 2 0.4 Tier II
7 NC_000003.12 3 17684230 - TBC1D5 NNNCCTTTGATTTATATACA NGG 2 0.3665 Tier II
8 NC_000001.11 1 171534101 + PRRC2C NNNGTTTTTATTTATATGCA NGG 2 0.3394 Tier II
9 NC_000010.11 10 99723548 - COX15 NNNGCTTTGATTTAAAAGCA NGG 2 0.3088 Tier II
10 NC_000005.10 5 31222348 - CDH6 NNNTCTCTGATTTATATGCA NGG 2 0.25 Tier II
11 NC_000008.11 8 19739895 + CSGALNACT1 NNNTCTTTCATTTATATGCA NGG 2 0.1958 Tier II
12 NC_000007.14 7 137579727 + DGKI NNNGCTCTGATTTACATGCA NGG 2 0.1875 Tier II
13 NC_000010.11 10 113580216 - HABP2 NNNGCTTTGAATTCTATGCA NGG 2 0.1607 Tier II
14 NC_000003.12 3 78717977 - ROBO1 NNNGCTTTGATTTATATGCT NAG 2 0.1556 Tier II
15 NC_000006.12 6 116074628 - FRK NNNGCTTTGATATATATGGA NGG 2 0.1 Tier II
16 NC_000016.10 16 76330360 + CNTNAP4 NNNTCTTTGATTTCTATGCA NGG 2 0.0779 Tier II
17 NC_000001.11 1 151434836 - POGZ NNNTCTTTGATTTATGTGCA NGG 2 0.0699 Tier II
18 NC_000013.11 13 110354103 + COL4A2 NNNCCTTTGATTTATATGGA NGG 2 0.0662 Tier II
19 NC_000015.10 15 100475614 - CERS3 NNNGCTTTGCTTTATGTGCA NGG 2 0.0641 Tier II
20 NC_000003.12 3 131618726 - CPNE4 NNNGCTTTGATTGATATGCC NGG 2 0.0593 Tier II
21 NC_000010.11 10 66005352 - CTNNA3 NNNGCTTTGATTTAAATGCA NGA 2 0.0402 Tier II
22 NC_000016.10 16 27947084 - GSG1L NNNGCTTGGATTTAGATGCA NGG 2 0.0367 Tier II
23 NC_000023.11 X 19358591 - PDHA1 NNNGCTTTGATTTAGATGTA NGG 2 0.0231 Tier II
24 NC_000002.12 2 110863865 - ACOXL NNNGCTTTGATTTCTATGCA NGA 2 0.0149 Tier II
25 NC_000009.12 9 124757741 + NR6A1 NNNGCTTTTATTTATATGCA NGC 2 0.0119 Tier II
26 NC_000001.11 1 197199051 - ZBTB41 NNNGCTTTGATTTATATGCC NGT 2 0.0037 Tier II
27 NC_000003.12 3 114845549 - ZBTB20 NNNGCCTTGATTTATTTGCA NGG 2 0.0 Tier II
28 NC_000014.9 14 72084985 + RGS6 NNNGCTTGGATTTATTTGCA NGG 2 0.0 Tier II
29 NC_000023.11 X 96786970 + DIAPH2 NNNGCTTTGATTTATATGCA NAT 2 0.0 Tier II
30 NC_000001.11 1 78062031 - GIPC2 NNNGCTTTGATTTATCTCCA NGG 2 0.0 Tier II
31 NC_000002.12 2 199403605 - SATB2 NNNGCTTTGCTTTATTTGCA NGG 2 0.0 Tier II
32 NC_000002.12 2 50530868 + NRXN1 NNNGCTTTTATTTATCTGCA NGG 2 0.0 Tier II
33 NC_000016.10 16 53150701 + CHD9 NNNGCTTTTATTTATCTGCA NGG 2 0.0 Tier II
34 NC_000009.12 9 22120550 - CDKN2B-AS1 NNNGCTTTGATCTATATGCT NGG 2 0.5684 Tier III
35 NC_000008.11 8 15394395 - LOC124902059 NNNGCTTAGATTTATATTCA NGG 2 0.5333 Tier III
36 NC_000005.10 5 61694057 - LINC03122 NNNGCATTGATTTATATGTA NGG 2 0.4 Tier III
37 NC_000005.10 5 61694057 - LINC03152 NNNGCATTGATTTATATGTA NGG 2 0.4 Tier III
38 NC_000014.9 14 81621942 - LINC01467 NNNACTTTGCTTTATATGCA NGG 2 0.3 Tier III
39 NC_000007.14 7 25379430 + LOC124901604 NNNGGTTTGATTTATATGTA NGG 2 0.2769 Tier III
40 NC_000004.12 4 28119572 + LOC105374557 NNNGCTTTGATTTATATCAA NGG 2 0.2041 Tier III
41 NC_000005.10 5 111542334 - STARD4-AS1 NNNGCTTTGATTTATATGCT NTG 2 0.0234 Tier III
42 NC_000015.10 15 53032465 - LOC107983981 NNNGCTTTGCTTTATATGCA NGA 2 0.0231 Tier III
43 NC_000006.12 6 66054547 + LOC105377839 NNNGCTTTGATTTATAGGGA NGG 2 0.0147 Tier III
44 NC_000006.12 6 66054547 + LOC105377840 NNNGCTTTGATTTATAGGGA NGG 2 0.0147 Tier III
45 NC_000009.12 9 124757741 + LOC124900274 NNNGCTTTTATTTATATGCA NGC 2 0.0119 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 95870068 + Atr NNNGCTCTGATGTATATGCA NGG 2 0.3438 Tier I
2 NC_000067.6 1 25597791 + Adgrb3 NNNGCATTGATCTATATGCA NGG 2 0.8211 Tier II
3 NC_000076.6 10 87204827 + 1700113H08Rik NNNGATTTGTTTTATATGCA NGG 2 0.5042 Tier II
4 NC_000075.6 9 100846489 + Stag1 NNNGCTTGGATTTATATTCA NGG 2 0.4889 Tier II
5 NC_000068.7 2 76495095 - Osbpl6 NNNGCTTAGATTTTTATGCA NGG 2 0.4267 Tier II
6 NC_000074.6 8 92866071 + Lpcat2 NNNGCTTTTATTCATATGCA NGG 2 0.4211 Tier II
7 NC_000071.6 5 127324197 + Tmem132c NNNGCTGTGATTTATACGCA NGG 2 0.4152 Tier II
8 NC_000069.6 3 138320989 - Adh6a NNNGCTTAGATTTATATCCA NGG 2 0.381 Tier II
9 NC_000079.6 13 68910965 + Adcy2 NNNGCTTTGATTTTTATTCA NGG 2 0.3556 Tier II
10 NC_000070.6 4 113536910 - Skint5 NNNGCTTTGATATATATGAA NGG 2 0.3429 Tier II
11 NC_000076.6 10 22187433 - Raet1e NNNCTTTTGATTTATATGCA NGG 2 0.3369 Tier II
12 NC_000070.6 4 8218963 - Car8 NNNGCTTTGATTTTTATGCT NGG 2 0.32 Tier II
13 NC_000071.6 5 22961210 + Lhfpl3 NNNCCTTTGATTTATATGCT NGG 2 0.3176 Tier II
14 NC_000085.6 19 8904823 - Ganab NNNGCTTTGATTTAAAAGCA NGG 2 0.3088 Tier II
15 NC_000083.6 17 5574264 + Zdhhc14 NNNTCTTTGATTTATACGCA NGG 2 0.2567 Tier II
16 NC_000081.6 15 13143007 + Cdh6 NNNTCTCTGATTTATATGCA NGG 2 0.25 Tier II
17 NC_000072.6 6 25671973 - Gpr37 NNNGCTTTGATCTATATGCA NAG 2 0.2456 Tier II
18 NC_000086.7 X 86865506 - Il1rapl1 NNNGCTTTGCTTTATATACA NGG 2 0.2308 Tier II
19 NC_000081.6 15 80516580 + Enthd1 NNNGCTTTGATTTATATGAA NAG 2 0.1111 Tier II
20 NC_000083.6 17 64710295 - Man2a1 NNNGCTTTGCTTTATATGCA NAG 2 0.0864 Tier II
21 NC_000067.6 1 25095001 - Adgrb3 NNNGCTTTGATTTGTAGGCA NGG 2 0.0549 Tier II
22 NC_000080.6 14 93686427 + Pcdh9 NNNGCTTTAATTTATATGCA NGA 2 0.0446 Tier II
23 NC_000083.6 17 67118128 - Ptprm NNNGCTTTGAATTAGATGCA NGG 2 0.0375 Tier II
24 NC_000073.6 7 50023260 - Nell1 NNNGCTTTTATTTATATGCA NGA 2 0.037 Tier II
25 NC_000074.6 8 15962512 - Csmd1 NNNGCTTTGATTTAGATGCA NTG 2 0.0019 Tier II
26 NC_000083.6 17 80699664 - Map4k3 NNNGCTTTGATTTTTTTGCA NGG 2 0.0 Tier II
27 NC_000067.6 1 88093766 - Ugt1a10 NNNGTTTTGATTTATCTGCA NGG 2 0.0 Tier II
28 NC_000067.6 1 88093766 - Ugt1a9 NNNGTTTTGATTTATCTGCA NGG 2 0.0 Tier II
29 NC_000067.6 1 88093766 - Ugt1a8 NNNGTTTTGATTTATCTGCA NGG 2 0.0 Tier II
30 NC_000070.6 4 128637946 + Gm12958 NNNGCTCTGATTCATATGCA NGG 2 0.5428 Tier III
31 NC_000080.6 14 79744168 - LOC115485928 NNNAATTTGATTTATATGCA NGG 2 0.5143 Tier III
32 NC_000073.6 7 142850813 - Gm6471 NNNGATTTGATATATATGCA NGG 2 0.4571 Tier III
33 NC_000073.6 7 142850813 - Gm39117 NNNGATTTGATATATATGCA NGG 2 0.4571 Tier III
34 NC_000071.6 5 39544588 - 4930513D17Rik NNNGCTTAGATTTATATGAA NGG 2 0.3429 Tier III
35 NC_000076.6 10 22187433 - Gm26740 NNNCTTTTGATTTATATGCA NGG 2 0.3369 Tier III
36 NC_000076.6 10 22377594 - Gm34607 NNNCTTTTGATTTATATGCA NGG 2 0.3369 Tier III
37 NC_000086.7 X 155625210 - Gm15155 NNNGCTTTGATTAATATGCA NGT 2 0.0112 Tier III
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Other clones with same target sequence:

(none)