Construct: sgRNA BRDN0001147595
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTAATCCTAAGTGGACCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4K2A (55361)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76822
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
97651007 |
+ |
PI4K2A |
NNNATCCTAAGTGGACCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
23574049 |
- |
SLC25A37 |
NNNATCTTAAGTGGACCAAA |
NGG |
2 |
0.7617 |
Tier II |
| 3 |
NC_000003.12 |
3 |
16361457 |
+ |
RFTN1 |
NNNATACTAAGTGGGCCAAG |
NGG |
2 |
0.6036 |
Tier II |
| 4 |
NC_000008.11 |
8 |
30396464 |
- |
RBPMS |
NNNATCCTAAGTGGACAAAA |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000005.10 |
5 |
35813574 |
- |
SPEF2 |
NNNATGCTAAGTGAACCAAG |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000010.11 |
10 |
123765704 |
+ |
CPXM2 |
NNNATCCTAACTGAACCAAG |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000013.11 |
13 |
101971751 |
- |
FGF14 |
NNNATCCTAGGTGGCCCAAG |
NGG |
2 |
0.1515 |
Tier II |
| 8 |
NC_000002.12 |
2 |
26340371 |
- |
ADGRF3 |
NNNATCCTAAGTGGAACAAG |
NGA |
2 |
0.0694 |
Tier II |
| 9 |
NC_000017.11 |
17 |
14845017 |
+ |
LINC02096 |
NNNATCCTAAGAGAACCAAG |
NGG |
2 |
0.6 |
Tier III |
| 10 |
NC_000008.11 |
8 |
16694741 |
+ |
LOC101929028 |
NNNATCATAAGTGGACAAAG |
NGG |
2 |
0.35 |
Tier III |
| 11 |
NC_000017.11 |
17 |
53434610 |
+ |
LOC645163 |
NNNATCCTAAGTGGATCAAG |
NGG |
1 |
0.3077 |
Tier III |
| 12 |
NC_000013.11 |
13 |
87578801 |
- |
MIR4500HG |
NNNATCCTAAGGGGACCAAG |
NAG |
2 |
0.1296 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
42100703 |
+ |
Pi4k2a |
NNNACCCTAAGTGGACCAAG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
25565013 |
- |
Fgfr1 |
NNNATCCTAAGAGGACCAAT |
NGG |
2 |
0.56 |
Tier II |
| 3 |
NC_000082.6 |
16 |
33859968 |
- |
Itgb5 |
NNNATCCTCAGTAGACCAAG |
NGG |
2 |
0.5275 |
Tier II |
| 4 |
NC_000083.6 |
17 |
34472336 |
+ |
Btnl4 |
NNNATCCTAAGGGGACCAAA |
NGG |
2 |
0.4688 |
Tier II |
| 5 |
NC_000077.6 |
11 |
67348080 |
+ |
Myh13 |
NNNATCCAAAGTGGCCCAAG |
NGG |
2 |
0.2182 |
Tier II |
| 6 |
NC_000071.6 |
5 |
8628703 |
+ |
Abcb1a |
NNNATCCTAAGAGTACCAAG |
NGG |
2 |
0.2133 |
Tier II |
| 7 |
NC_000069.6 |
3 |
146885729 |
+ |
Ttll7 |
NNNATCCTAAGTGGGGCAAG |
NGG |
2 |
0.1 |
Tier II |
| 8 |
NC_000077.6 |
11 |
94931085 |
+ |
Gm46293 |
NNNATCCCAAGAGGACCAAG |
NGG |
2 |
0.8 |
Tier III |
Other clones with same target sequence:
(none)
