Construct: sgRNA BRDN0001147596

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAAGGATGGAACAAGACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMPK1 (51727)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77300

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 47373052 + CMPK1 NNNAGGATGGAACAAGACCA NGG 0 1.0 Tier I
2 NC_000015.10 15 73894802 + TBC1D21 NNNTGGATGGAACAAGACCA NGG 1 0.6364 Tier II
3 NC_000011.10 11 43933430 - ACCSL NNNGGGAGGGAACAAGACCA NGG 2 0.4583 Tier II
4 NC_000011.10 11 11586039 + GALNT18 NNNAGGATGGAACAAGAACG NGG 2 0.4118 Tier II
5 NC_000001.11 1 159294404 - FCER1A NNNAGGATGAAACAAGACAA NGG 2 0.4 Tier II
6 NC_000006.12 6 89830538 - CASP8AP2 NNNAGAATGGAAAAAGACCA NGG 2 0.3846 Tier II
7 NC_000001.11 1 14293583 - KAZN NNNAGAATGGACCAAGACCA NGG 2 0.2632 Tier II
8 NC_000002.12 2 225567726 + NYAP2 NNNAGGATGGTACAAGAACA NGG 2 0.1657 Tier II
9 NC_000004.12 4 148254112 - NR3C2 NNNAGGATGGACCTAGACCA NGG 2 0.1404 Tier II
10 NC_000004.12 4 139023179 - NOCT NNNAGGATGCATCAAGACCA NGG 2 0.1333 Tier II
11 NC_000019.10 19 54374015 + LAIR1 NNNATGATGCAACAAGACCA NGG 2 0.12 Tier II
12 NC_000005.10 5 33767873 + ADAMTS12 NNNAAGATGGAACAAGAGCA NGG 2 0.1156 Tier II
13 NC_000021.9 21 44807620 - SUMO3 NNNAGGATGGAAGAAGATCA NGG 2 0.0877 Tier II
14 NC_000002.12 2 42529388 - MTA3 NNNATGATGGACCAAGACCA NGG 2 0.0789 Tier II
15 NC_000014.9 14 36743650 + SLC25A21 NNNAGGATGGAACAAGACAA NGA 2 0.0298 Tier II
16 NC_000008.11 8 67254614 + ARFGEF1 NNNAGGATGGCACAAGACCA NGA 2 0.0278 Tier II
17 NC_000001.11 1 2700691 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
18 NC_000001.11 1 2761642 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
19 NC_000001.11 1 2764979 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
20 NC_000001.11 1 2768694 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
21 NC_000001.11 1 2768853 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
22 NC_000001.11 1 2772241 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
23 NC_000001.11 1 2776319 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
24 NC_000001.11 1 2776438 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
25 NC_000001.11 1 2776597 + TTC34 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier II
26 NC_000005.10 5 139053650 - SIL1 NNNAGGATGGAAAAAGACCA NTG 2 0.015 Tier II
27 NC_000020.11 20 3797244 - CDC25B NNNAGGATGGACCAAGACCA NGT 2 0.0042 Tier II
28 NC_000010.11 10 1450793 - ADARB2 NNNAGGCTGGAACAACACCA NGG 2 0.0 Tier II
29 NC_000011.10 11 36824974 - LOC107984326 NNNAGAGTGGAACAAGACCA NGG 2 0.7059 Tier III
30 NC_000011.10 11 125696806 - LOC105369551 NNNAGCATGGAACAAGACCA NGG 1 0.6818 Tier III
31 NC_000001.11 1 210231675 - SERTAD4-AS1 NNNAGGAAGGAACAAGATCA NGG 2 0.5143 Tier III
32 NC_000011.10 11 43933430 - LOC124902663 NNNGGGAGGGAACAAGACCA NGG 2 0.4583 Tier III
33 NC_000001.11 1 38379191 + LOC105378657 NNNAGTATAGAACAAGACCA NGG 2 0.4286 Tier III
34 NC_000001.11 1 14293583 - LOC124903847 NNNAGAATGGACCAAGACCA NGG 2 0.2632 Tier III
35 NC_000015.10 15 36314422 - LOC105370767 NNNAGGATGGATCAAGACCA NAG 2 0.0864 Tier III
36 NC_000001.11 1 2764979 + LOC107985732 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
37 NC_000001.11 1 2764979 + LOC112268220 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
38 NC_000001.11 1 2768694 + LOC105378598 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
39 NC_000001.11 1 2768853 + LOC105378598 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
40 NC_000001.11 1 2772241 + LOC105378598 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
41 NC_000001.11 1 2772241 + LOC107984904 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
42 NC_000001.11 1 2776319 + LOC105378598 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
43 NC_000001.11 1 2776438 + LOC105378598 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
44 NC_000001.11 1 2776597 + LOC105378598 NNNAGCATGGAACAAGACCA NTG 2 0.0266 Tier III
45 NC_000010.11 10 38212330 + CCNYL4 NNNAGGATGGAACAAGACAA NGT 2 0.0069 Tier III
46 NC_000010.11 10 42410171 - CCNYL2 NNNAGGATGGAACAAGACAA NGT 2 0.0069 Tier III
47 NC_000023.11 X 103553744 + LINC02589 NNNAGGATGGAAAAATACCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 114964939 - Cmpk1 NNNAGGTTGGAACAAAACCA NGG 2 0.4375 Tier I
2 NC_000068.7 2 119293042 + Vps18 NNNGGGATGGAACAAGGCCA NGG 2 0.1103 Tier I
3 NC_000084.6 18 79051090 + Setbp1 NNNAGGAAGGAACAAAACCA NGG 2 0.8 Tier II
4 NC_000078.6 12 105730045 + Ak7 NNNAGGAAGGAACAAGACCT NGG 2 0.48 Tier II
5 NC_000068.7 2 21505844 - Gpr158 NNNGGGATGGAACAGGACCA NGG 2 0.4063 Tier II
6 NC_000068.7 2 143677096 + Pcsk2 NNNAGGATGGAACAAAACCA NAG 2 0.2593 Tier II
7 NC_000068.7 2 102095011 + Ldlrad3 NNNAGGCTGGCACAAGACCA NGG 2 0.175 Tier II
8 NC_000074.6 8 33671382 + Gsr NNNAGGATGGAACAAGACCT NAG 2 0.1556 Tier II
9 NC_000067.6 1 128123888 - R3hdm1 NNNATGATGGAAAAAGACCA NGG 2 0.1154 Tier II
10 NC_000067.6 1 65118585 - D630023F18Rik NNNAGGAAGGAACAAGAGCA NGG 2 0.1067 Tier II
11 NC_000076.6 10 119665735 - Grip1 NNNAGGAGGGAAGAAGACCA NGG 2 0.1 Tier II
12 NC_000068.7 2 14708406 - Cacnb2 NNNAGGATGGAACAGGACCA NCG 2 0.0696 Tier II
13 NC_000072.6 6 92524580 + Prickle2 NNNAGGATGGACCAAGACCC NGG 2 0.0598 Tier II
14 NC_000073.6 7 127540589 - Srcap NNNAGGATGGAAGAAGTCCA NGG 2 0.0182 Tier II
15 NC_000068.7 2 65148109 + Cobll1 NNNAGCATGGAACAACACCA NGG 2 0.0 Tier II
16 NC_000070.6 4 43271341 - Atp8b5 NNNAGGATGGGACAATACCA NGG 2 0.0 Tier II
17 NC_000071.6 5 69461470 + Gm5867 NNNAGGCTGGAACAAAACCA NGG 2 0.4375 Tier III
18 NC_000067.6 1 89360629 + C030007H22Rik NNNAGGATTGAACAAGAACA NGG 2 0.2872 Tier III
19 NC_000067.6 1 128123888 - Gm29383 NNNATGATGGAAAAAGACCA NGG 2 0.1154 Tier III
20 NC_000082.6 16 89657335 - Gm35500 NNNAGGATGGAACAGGGCCA NGG 2 0.1147 Tier III
21 NC_000067.6 1 65118585 - Gm36358 NNNAGGAAGGAACAAGAGCA NGG 2 0.1067 Tier III
22 NC_000071.6 5 3298015 + Gm36470 NNNAGGGTGGAAGAAGACCA NGG 2 0.0963 Tier III
23 NC_000077.6 11 44038517 + Gm12153 NNNAGGATGGAACAAGAACA NGA 2 0.0374 Tier III
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Other clones with same target sequence:

(none)