Construct: sgRNA BRDN0001147601
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCGCCGAAACTCTTTCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80199
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
2192577 |
- |
SMARCA2 |
NNNCGCCAAATCTCTTTCCG |
NGG |
2 |
0.3077 |
Tier II |
| 2 |
NC_000009.12 |
9 |
2192577 |
- |
LOC107987043 |
NNNCGCCAAATCTCTTTCCG |
NGG |
2 |
0.3077 |
Tier III |
| 3 |
NC_000016.10 |
16 |
80422132 |
+ |
DYNLRB2-AS1 |
NNNCACCGAAACTCCTTCCG |
NGG |
2 |
0.2364 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
42982026 |
- |
Ppp2r2b |
NNNCGCCGATACTCTTTCTG |
NGG |
2 |
0.4072 |
Tier II |
| 2 |
NC_000076.6 |
10 |
36992943 |
- |
Hdac2 |
NNNCTCTGAAACTCTTTCCG |
NGG |
2 |
0.2438 |
Tier II |
Other clones with same target sequence:
(none)
