Construct: sgRNA BRDN0001147603

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTACTCACATTCCTCAAGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTPCR (84284)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76876

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 232956435 - NTPCR NNNCTCACATTCCTCAAGAC NGG 0 1.0 Tier I
2 NC_000001.11 1 166922425 - ILDR2 NNNCTCACATTTGTCAAGAC NGG 2 0.0734 Tier I
3 NC_000010.11 10 50817664 - A1CF NNNCTCATCTTCCTCAAGAC NGG 2 0.5 Tier II
4 NC_000008.11 8 55395896 + XKR4 NNNCTCACAGTTCTCAAGAC NGG 2 0.2692 Tier II
5 NC_000003.12 3 177025990 + TBL1XR1 NNNCACACATTCCTCAACAC NGG 2 0.2381 Tier II
6 NC_000010.11 10 62464069 - ZNF365 NNNCTCTCATTGCTCAAGAC NGG 2 0.1944 Tier II
7 NC_000005.10 5 155957431 + SGCD NNNCTCCCATTCCTCAAGGC NGG 2 0.1641 Tier II
8 NC_000004.12 4 151614724 - FHIP1A NNNCTCACATTCCTCAAACC NGG 2 0.1432 Tier II
9 NC_000003.12 3 125127130 + SLC12A8 NNNCTCAGATTCCTAAAGAC NGG 2 0.1429 Tier II
10 NC_000012.12 12 119495728 + CCDC60 NNNCTCACTTTCCTCAAGCC NGG 2 0.1241 Tier II
11 NC_000010.11 10 47732000 - ANXA8 NNNCTCAGATTCCTCAGGAC NGG 2 0.1134 Tier II
12 NC_000006.12 6 132817573 + RPS12 NNNCTCACATTCCTCATGTC NGG 2 0.0718 Tier II
13 NC_000014.9 14 59255450 - DAAM1 NNNCTCACATTCCTCATGTC NGG 2 0.0718 Tier II
14 NC_000012.12 12 132495118 - FBRSL1 NNNCTCACATTCCCCACGAC NGG 2 0.0504 Tier II
15 NC_000003.12 3 33091467 + GLB1 NNNCTCACATTCCCCAGGAC NGG 2 0.0504 Tier II
16 NC_000003.12 3 33091467 + TMPPE NNNCTCACATTCCCCAGGAC NGG 2 0.0504 Tier II
17 NC_000003.12 3 7171129 + GRM7 NNNCTCACATTCCTGAAGTC NGG 2 0.0269 Tier II
18 NC_000001.11 1 41032137 - SCMH1 NNNCTCACAGTCCTGAAGAC NGG 2 0.025 Tier II
19 NC_000006.12 6 20154799 + MBOAT1 NNNCTCACATTCCCTAAGAC NGG 2 0.019 Tier II
20 NC_000023.11 X 97140292 - DIAPH2 NNNCTCACATTCCTAAAGAG NGG 2 0.0131 Tier II
21 NC_000011.10 11 24989366 + LUZP2 NNNCTCACATTCCTAAAGAC NGT 2 0.0036 Tier II
22 NC_000006.12 6 57206698 - RAB23 NNNCTCACATTCCTCAAGAC NTA 2 0.0 Tier II
23 NC_000019.10 19 4058584 + ZBTB7A NNNCTCACATTCCTCCAGGC NGG 2 0.0 Tier II
24 NC_000001.11 1 165894460 + UCK2 NNNCTGACATTCCTCTAGAC NGG 2 0.0 Tier II
25 NC_000012.12 12 73003292 - LOC105369838 NNNCTCACATTCCTCAAGTC NGG 1 0.5385 Tier III
26 NC_000005.10 5 172630423 - LOC107986478 NNNCTCACATTCTTCAAGTC NGG 2 0.3769 Tier III
27 NC_000006.12 6 113365840 - LOC107986637 NNNCACACATTCCTCAAAAC NGG 2 0.3462 Tier III
28 NC_000003.12 3 55308153 + LOC124906243 NNNCTCACAGTTCTCAAGAC NGG 2 0.2692 Tier III
29 NC_000014.9 14 22481246 - TRA NNNCTGACATTTCTCAAGAC NGG 2 0.2692 Tier III
30 NC_000014.9 14 22481246 - TRD-AS1 NNNCTGACATTTCTCAAGAC NGG 2 0.2692 Tier III
31 NC_000006.12 6 167240757 + LOC102724220 NNNCTCAAATTCCTCAAGGC NGG 2 0.2438 Tier III
32 NC_000012.12 12 76296628 + LNCOG NNNCTCACATGCCTCAAGTC NGG 2 0.2154 Tier III
33 NC_000023.11 X 103430503 - LL0XNC01-250H12.3 NNNCTCACATGCCTCAAGTC NGG 2 0.2154 Tier III
34 NC_000012.12 12 119495728 + PRKAB1-AS1 NNNCTCACTTTCCTCAAGCC NGG 2 0.1241 Tier III
35 NC_000010.11 10 46609691 + LOC105378289 NNNCTCAGATTCCTCAGGAC NGG 2 0.1134 Tier III
36 NC_000010.11 10 47732000 - LOC124902422 NNNCTCAGATTCCTCAGGAC NGG 2 0.1134 Tier III
37 NC_000015.10 15 96691859 - LOC105371002 NNNTTCACATTCCTCAAGAC NTG 2 0.0312 Tier III
38 NC_000001.11 1 41032137 - SLFNL1-AS1 NNNCTCACAGTCCTGAAGAC NGG 2 0.025 Tier III
39 NC_000002.12 2 140089719 + LINC01853 NNNCTCACTTTCCTCAAGAC NGT 2 0.0097 Tier III
40 NC_000014.9 14 88042334 + HISLA NNNCTCACTTTCCTCAAGAC NGT 2 0.0097 Tier III
41 NC_000001.11 1 101672499 - LINC01709 NNNCTCACATTTCTCTAGAC NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 10427712 - Greb1l NNNCTAACATTCCTCAACAC NGG 2 0.4422 Tier II
2 NC_000073.6 7 73393919 - Rgma NNNCCCACATTCCTCAAGGC NGG 2 0.375 Tier II
3 NC_000076.6 10 74283271 + Pcdh15 NNNCTTACATTCATCAAGAC NGG 2 0.3571 Tier II
4 NC_000076.6 10 89587324 + Slc17a8 NNNCTGACATTCCTCAAAAC NGG 2 0.3462 Tier II
5 NC_000075.6 9 46294594 + Bud13 NNNGTCAGATTCCTCAAGAC NGG 2 0.3214 Tier II
6 NC_000075.6 9 100470558 - Il20rb NNNCTCTCATTCTTCAAGAC NGG 2 0.3062 Tier II
7 NC_000078.6 12 85696464 - Batf NNNCACACATTCCTCAAGTC NGG 2 0.2692 Tier II
8 NC_000078.6 12 102161313 + Slc24a4 NNNCTCACAATCCTAAAGAC NGG 2 0.1905 Tier II
9 NC_000069.6 3 154935544 - Tnni3k NNNCTCACATTTCTCAAGAT NGG 2 0.1615 Tier II
10 NC_000077.6 11 106243792 + Ddx42 NNNCACACATTCCTCAAGAC NAG 2 0.1296 Tier II
11 NC_000067.6 1 38457521 - Aff3 NNNCACACATTCCTCAGGAC NGG 2 0.0882 Tier II
12 NC_000077.6 11 80814463 - Tmem98 NNNCTGACATTCCTCATGAC NGG 2 0.0667 Tier II
13 NC_000078.6 12 44546925 - Nrcam NNNCTCACATTCCTGAAGAC NGG 1 0.05 Tier II
14 NC_000068.7 2 125364919 - Fbn1 NNNCTCACATTCTTCAAGAC NGA 2 0.0486 Tier II
15 NC_000067.6 1 115701233 + Cntnap5a NNNCTCACATTCTTTAAGAC NGG 2 0.0467 Tier II
16 NC_000079.6 13 99217199 + Zfp366 NNNCTCACTTTCCTCAAGAG NGG 2 0.0353 Tier II
17 NC_000072.6 6 97937298 + Mitf NNNCTCACATTCCTCAACAG NGG 2 0.028 Tier II
18 NC_000068.7 2 13451103 - Cubn NNNCTCACATTCCTCAAGGC NGA 2 0.026 Tier II
19 NC_000080.6 14 48531369 + Tmem260 NNNCTCACGTTCCTCAAGAC NGC 2 0.0148 Tier II
20 NC_000068.7 2 76024201 + Pde11a NNNCTCACATTCCTCAGGAC NGA 2 0.0123 Tier II
21 NC_000074.6 8 32320325 + Nrg1 NNNCTCACATTCCTGAAGCC NGG 2 0.0103 Tier II
22 NC_000068.7 2 39153293 - Scai NNNCTCACATTCCTCTACAC NGG 2 0.0 Tier II
23 NC_000068.7 2 97623202 + Lrrc4c NNNCTCACATTCCTCTACAC NGG 2 0.0 Tier II
24 NC_000070.6 4 25217915 + Fhl5 NNNCTCACATTCCTCTACAC NGG 2 0.0 Tier II
25 NC_000081.6 15 43120325 + Rspo2 NNNCTCACATTCCTCTACAC NGG 2 0.0 Tier II
26 NC_000086.7 X 162622294 - Reps2 NNNCTCACATTCCTCTACAC NGG 2 0.0 Tier II
27 NC_000074.6 8 58854253 - Galntl6 NNNCTCACATTCCTCTATAC NGG 2 0.0 Tier II
28 NC_000074.6 8 125463837 + Sipa1l2 NNNCTCACATTGCTCCAGAC NGG 2 0.0 Tier II
29 NC_000073.6 7 73393919 - LOC115486533 NNNCCCACATTCCTCAAGGC NGG 2 0.375 Tier III
30 NC_000083.6 17 46351757 + BC040756 NNNCTCAAATTCCTCAAGCC NGG 2 0.1345 Tier III
31 NC_000081.6 15 61723937 + D030024E09Rik NNNGTCACATTCCTCAAGCC NGG 2 0.1034 Tier III
32 NC_000077.6 11 80814463 - Gm31228 NNNCTGACATTCCTCATGAC NGG 2 0.0667 Tier III
Download CSV

Other clones with same target sequence:

(none)