Construct: sgRNA BRDN0001147606
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGACGGAGCCGTAGGCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK11 (5600)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77482
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
50270193 |
+ |
MAPK11 |
NNNGACGGAGCCGTAGGCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
15944966 |
+ |
ZBTB17 |
NNNGACGGAGCCGTAGGCCT |
NGG |
2 |
0.1345 |
Tier I |
| 3 |
NC_000017.11 |
17 |
44005748 |
- |
NAGS |
NNNGAGGGAGCCGTAGGGGC |
NGG |
2 |
0.0667 |
Tier I |
| 4 |
NC_000009.12 |
9 |
133429103 |
- |
ADAMTS13 |
NNNGACGGAGCTGTGGGCGC |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000009.12 |
9 |
133428924 |
- |
ADAMTS13 |
NNNGACGGAGCGGTGGGCGC |
NGG |
2 |
0.2889 |
Tier II |
| 6 |
NC_000018.10 |
18 |
812458 |
- |
YES1 |
NNNGACGGGGGCGTAGGCGC |
NGG |
2 |
0.1667 |
Tier II |
| 7 |
NC_000016.10 |
16 |
138325 |
+ |
NPRL3 |
NNNGACGGAGCCGGAGGCGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000016.10 |
16 |
138357 |
+ |
NPRL3 |
NNNGACGGAGCCGGAGGCGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000015.10 |
15 |
45167319 |
+ |
SHF |
NNNGACGGCGCCGGAGGCGC |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
89149414 |
+ |
Mapk11 |
NNNGACTGAGCCGTAGGCGC |
NGG |
1 |
0.5714 |
Tier I |
| 2 |
NC_000075.6 |
9 |
31360938 |
- |
Prdm10 |
NNNGACGGAGCCGCAGGGGC |
NGG |
2 |
0.0381 |
Tier I |
| 3 |
NC_000079.6 |
13 |
23555394 |
- |
H1f3 |
NNNGAAGGAGCCGGAGGCGC |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000079.6 |
13 |
23622197 |
+ |
H1f4 |
NNNGAAGGAGCCGGAGGCGC |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000083.6 |
17 |
84371936 |
- |
Thada |
NNNGACGGAGCCGTAGCCGC |
NGG |
1 |
0.2353 |
Tier II |
| 6 |
NC_000070.6 |
4 |
73498535 |
+ |
Gm11488 |
NNNGACGGAGCCGTCGGCGA |
NGG |
2 |
0.1364 |
Tier III |
Other clones with same target sequence:
(none)
