Construct: sgRNA BRDN0001147607
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATACATGAAAACGTAGCCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1A1L (122011)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77383
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
37104635 |
+ |
CSNK1A1L |
NNNCATGAAAACGTAGCCTA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
63823872 |
+ |
HERC1 |
NNNAATGAAAACGTAGCCTA |
NGG |
1 |
0.8421 |
Tier II |
| 3 |
NC_000002.12 |
2 |
161937830 |
+ |
SLC4A10 |
NNNCCTGAAAACATAGCCTA |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000006.12 |
6 |
157901290 |
- |
SNX9 |
NNNAATGAAAAGGTAGCCTA |
NGG |
2 |
0.3743 |
Tier II |
| 5 |
NC_000019.10 |
19 |
53548619 |
- |
ZNF331 |
NNNAATGAAAAGGTAGCCTA |
NGG |
2 |
0.3743 |
Tier II |
| 6 |
NC_000013.11 |
13 |
40206963 |
- |
LOC124903162 |
NNNCATGAATACGCAGCCTA |
NGG |
2 |
0.2521 |
Tier II |
| 7 |
NC_000022.11 |
22 |
44842968 |
- |
ARHGAP8 |
NNNAATGAAAACGTAGCCCA |
NGG |
2 |
0.2323 |
Tier II |
| 8 |
NC_000022.11 |
22 |
44842968 |
- |
PRR5-ARHGAP8 |
NNNAATGAAAACGTAGCCCA |
NGG |
2 |
0.2323 |
Tier II |
| 9 |
NC_000003.12 |
3 |
128338657 |
- |
EEFSEC |
NNNCCTGAAAACGTAGCCAA |
NGG |
2 |
0.1429 |
Tier II |
| 10 |
NC_000001.11 |
1 |
108254009 |
- |
NBPF4 |
NNNCATGAAAAGGCAGCCTA |
NGG |
2 |
0.127 |
Tier II |
| 11 |
NC_000001.11 |
1 |
108440362 |
+ |
NBPF6 |
NNNCATGAAAAGGCAGCCTA |
NGG |
2 |
0.127 |
Tier II |
| 12 |
NC_000013.11 |
13 |
40206963 |
- |
LINC00548 |
NNNCATGAATACGCAGCCTA |
NGG |
2 |
0.2521 |
Tier III |
| 13 |
NC_000005.10 |
5 |
152071509 |
+ |
LINC01933 |
NNNCATGAAAACGAAGCCTA |
NGA |
2 |
0.043 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
112146442 |
- |
Skint4 |
NNNTATGAAAAAGTAGCCTA |
NGG |
2 |
0.5714 |
Tier II |
| 2 |
NC_000071.6 |
5 |
135116747 |
- |
Mlxipl |
NNNCATGAAAACGTGGCCTG |
NGG |
2 |
0.4971 |
Tier II |
| 3 |
NC_000067.6 |
1 |
98490930 |
- |
Slco6d1 |
NNNCATGAAAATGTAGACTA |
NGG |
2 |
0.2513 |
Tier II |
| 4 |
NC_000067.6 |
1 |
181124944 |
+ |
Nvl |
NNNCATGAGAACTTAGCCTA |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000075.6 |
9 |
117015620 |
+ |
Rbms3 |
NNNCATGAAAATGTAGCCAA |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000069.6 |
3 |
153633771 |
- |
St6galnac3 |
NNNCATGAAAGCGTAGGCTA |
NGG |
2 |
0.0382 |
Tier II |
| 7 |
NC_000071.6 |
5 |
30889553 |
- |
Agbl5 |
NNNTATGAAAACGTAGCCTA |
NGC |
2 |
0.0178 |
Tier II |
| 8 |
NC_000072.6 |
6 |
41202522 |
+ |
Tcrb |
NNNCATGAGAAGGTAGCCTA |
NGG |
2 |
0.2963 |
Tier III |
| 9 |
NC_000071.6 |
5 |
119079554 |
- |
Gm31034 |
NNNCATGAAAAAGTAGCCTA |
NGT |
2 |
0.0115 |
Tier III |
| 10 |
NC_000080.6 |
14 |
67177830 |
+ |
Gm30438 |
NNNCATGAAAACGGGGCCTA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
