Construct: sgRNA BRDN0001147611
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCCCATTAGGCCCATTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK494 (124923)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77173
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
28613462 |
- |
RSKR |
NNNCCCATTAGGCCCATTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
25045269 |
+ |
KIAA1671 |
NNNCCCTCTAGGCCCATTAG |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000011.10 |
11 |
126384108 |
- |
ST3GAL4 |
NNNCCCATTAGTCCCATTAG |
NAG |
2 |
0.0997 |
Tier II |
| 4 |
NC_000005.10 |
5 |
31731967 |
+ |
PDZD2 |
NNNGCCATTAGGGCCATTAG |
NGG |
2 |
0.0682 |
Tier II |
| 5 |
NC_000002.12 |
2 |
219546300 |
- |
TMEM198 |
NNNGCCATTAGGCCCATTAG |
NGA |
2 |
0.0347 |
Tier II |
| 6 |
NC_000002.12 |
2 |
197995511 |
- |
PLCL1 |
NNNCCCATTAGGCCCCTTTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000017.11 |
17 |
28613462 |
- |
SPAG5-AS1 |
NNNCCCATTAGGCCCATTAG |
NGG |
0 |
1.0 |
Tier III |
| 8 |
NC_000012.12 |
12 |
73829498 |
- |
LINC02445 |
NNNCCAAATAGGCCCATTAG |
NGG |
2 |
0.7429 |
Tier III |
| 9 |
NC_000001.11 |
1 |
143934173 |
- |
LOC105371209 |
NNNCCCAGGAGGCCCATTAG |
NGG |
2 |
0.454 |
Tier III |
| 10 |
NC_000001.11 |
1 |
206165064 |
- |
LOC105372873 |
NNNCCCAGGAGGCCCATTAG |
NGG |
2 |
0.454 |
Tier III |
| 11 |
NC_000015.10 |
15 |
81958040 |
+ |
LINC01418 |
NNNCCCATTAGGCCCATTAT |
NAG |
2 |
0.1815 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
90783287 |
+ |
Anks1b |
NNNCCCATTAAGCCCATTAC |
NGG |
2 |
0.4286 |
Tier II |
| 2 |
NC_000072.6 |
6 |
115217672 |
+ |
Syn2 |
NNNCCCATAAGTCCCATTAG |
NGG |
2 |
0.3571 |
Tier II |
| 3 |
NC_000072.6 |
6 |
43392618 |
+ |
Tpk1 |
NNNCCCATTGGGCCCATTAG |
NGC |
2 |
0.0123 |
Tier II |
| 4 |
NC_000075.6 |
9 |
115493405 |
+ |
Gm39447 |
NNNCCAATTAGGCCAATTAG |
NGG |
2 |
0.2063 |
Tier III |
Other clones with same target sequence:
(none)
