Construct: sgRNA BRDN0001147613
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCACCTTAATGATCCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKI (9162)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76615
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
137619850 |
+ |
DGKI |
NNNCACCTTAATGATCCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
162021984 |
+ |
DPP4 |
NNNCACCTCTATGATCCAAG |
NGG |
2 |
0.8145 |
Tier II |
| 3 |
NC_000001.11 |
1 |
162681984 |
- |
DDR2 |
NNNCACCTAAATGTTCCAAG |
NGG |
2 |
0.4952 |
Tier II |
| 4 |
NC_000009.12 |
9 |
113321347 |
+ |
WDR31 |
NNNTACCTTAATGATCCATG |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000020.11 |
20 |
58406559 |
- |
VAPB |
NNNCACCTTGATGATCCAAC |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000003.12 |
3 |
141914126 |
+ |
ATP1B3 |
NNNCACATTAATGCTCCAAG |
NGG |
2 |
0.1607 |
Tier II |
| 7 |
NC_000009.12 |
9 |
15795860 |
+ |
CCDC171 |
NNNCACCTTAATGATCCAAG |
NAC |
2 |
0.0 |
Tier II |
| 8 |
NC_000010.11 |
10 |
115055642 |
- |
LOC107984272 |
NNNTAACTTAATGATCCAAG |
NGG |
2 |
0.7429 |
Tier III |
| 9 |
NC_000011.10 |
11 |
78427680 |
+ |
LINC02728 |
NNNCACCTTAATGATTCAAT |
NGG |
2 |
0.2154 |
Tier III |
| 10 |
NC_000008.11 |
8 |
7871016 |
- |
LOC124901865 |
NNNTACCTTAATGATCCAAG |
NAG |
2 |
0.2074 |
Tier III |
| 11 |
NC_000005.10 |
5 |
56770703 |
+ |
LOC105378979 |
NNNCACCTAAATGATCCCAG |
NGG |
2 |
0.1769 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
37063923 |
+ |
Dgki |
NNNCACCTTAATGATCCAGG |
NGG |
1 |
0.375 |
Tier I |
| 2 |
NC_000072.6 |
6 |
135772237 |
- |
Grin2b |
NNNCGCCTTCATGATCCAAG |
NGG |
2 |
0.24 |
Tier I |
| 3 |
NC_000081.6 |
15 |
41499489 |
- |
Oxr1 |
NNNCACCCTAATGAACCAAG |
NGG |
2 |
0.5789 |
Tier II |
| 4 |
NC_000069.6 |
3 |
65351129 |
+ |
Kcnab1 |
NNNCACCTTAATGATACAGG |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000080.6 |
14 |
19902037 |
+ |
Gng2 |
NNNCACCATAATCATCCAAG |
NGG |
2 |
0.3368 |
Tier II |
| 6 |
NC_000080.6 |
14 |
22989291 |
+ |
Lrmda |
NNNCACCTTAATGATCCTAG |
NAG |
2 |
0.1296 |
Tier II |
| 7 |
NC_000068.7 |
2 |
20435924 |
- |
Etl4 |
NNNCACCTTAATGATGCCAG |
NGG |
2 |
0.0293 |
Tier II |
| 8 |
NC_000071.6 |
5 |
91529109 |
- |
Parm1 |
NNNCACCTTGATGATCCAAG |
NTG |
2 |
0.0216 |
Tier II |
| 9 |
NC_000086.7 |
X |
17114087 |
- |
Gm4203 |
NNNGACCTTAATGATCCAGG |
NGG |
2 |
0.1875 |
Tier III |
| 10 |
NC_000080.6 |
14 |
45550735 |
- |
4930527F14Rik |
NNNCACCTTAATGATCTAAG |
NGA |
2 |
0.0324 |
Tier III |
| 11 |
NC_000080.6 |
14 |
45550735 |
- |
LOC115488341 |
NNNCACCTTAATGATCTAAG |
NGA |
2 |
0.0324 |
Tier III |
| 12 |
NC_000081.6 |
15 |
38446680 |
+ |
G930009F23Rik |
NNNCACCTTAATGAACCAAG |
NTG |
2 |
0.0226 |
Tier III |
Other clones with same target sequence:
(none)
