Construct: sgRNA BRDN0001147616

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGTCTTCCAGGTTACTGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDXK (8566)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76935

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000021.9 21 43750506 + PDXK NNNTCTTCCAGGTTACTGAG NGG 0 1.0 Tier I
2 NC_000005.10 5 70974274 + NAIP NNNTCTTCCAGGTTACTAAG NGA 2 0.0481 Tier I
3 NC_000011.10 11 108279530 + ATM NNNTCTTCCAGGTTACTGAA NGC 2 0.0208 Tier I
4 NC_000009.12 9 133373219 - SURF4 NNNTCTCCCAGGTTACTGAG NGG 1 0.6875 Tier II
5 NC_000007.14 7 116681160 - MET NNNTCTTCCTGGTAACTGAG NGG 2 0.5462 Tier II
6 NC_000016.10 16 71453967 - ZNF23 NNNCCTTACAGGTTACTGAG NGG 2 0.4206 Tier II
7 NC_000010.11 10 24091985 - KIAA1217 NNNTCTTCCATGTTAATGAG NGG 2 0.3846 Tier II
8 NC_000007.14 7 122003787 - PTPRZ1 NNNTCTACCACGTTACTGAG NGG 2 0.375 Tier II
9 NC_000001.11 1 210431732 + HHAT NNNTCTTCCAGGTTACTATG NGG 2 0.3728 Tier II
10 NC_000010.11 10 125644218 - TEX36 NNNTCTTCCAGCTTACTAAG NGG 2 0.3665 Tier II
11 NC_000006.12 6 166928945 - RNASET2 NNNTCTTCCAGGTTACTGGA NGG 2 0.3516 Tier II
12 NC_000010.11 10 80550242 - SH2D4B NNNTCTTCCAGGTCACTGAG NGG 1 0.2857 Tier II
13 NC_000011.10 11 16814730 + PLEKHA7 NNNTCTTCCAGCTTACTGTG NGG 2 0.2851 Tier II
14 NC_000011.10 11 7037730 - NLRP14 NNNTCTTCCAGTTAACTGAG NGG 2 0.2381 Tier II
15 NC_000010.11 10 130052781 - C10orf143 NNNTCTTCAAGGGTACTGAG NGG 2 0.2236 Tier II
16 NC_000011.10 11 92655031 + FAT3 NNNTCTTCCAGGTTTCTGAA NGG 2 0.1875 Tier II
17 NC_000017.11 17 44925007 - KIF18B NNNTCTTCCTGGTTTCTGAG NGG 2 0.1765 Tier II
18 NC_000010.11 10 79110494 + ZMIZ1 NNNTCTTCCAGGGAACTGAG NGG 2 0.1615 Tier II
19 NC_000002.12 2 214156208 + SPAG16 NNNTCTTCCAGGTTTCTGAT NGG 2 0.14 Tier II
20 NC_000004.12 4 112251552 - AP1AR NNNTCCTCCAGGTTAGTGAG NGG 2 0.1399 Tier II
21 NC_000020.11 20 43056837 + PTPRT NNNTGTTCCAGGTTTCTGAG NGG 2 0.12 Tier II
22 NC_000012.12 12 128539740 + TMEM132C NNNTCTTCTAGGTTACGGAG NGG 2 0.1029 Tier II
23 NC_000013.11 13 46096982 - CPB2 NNNTCTTCCAGGTTCTTGAG NGG 2 0.0839 Tier II
24 NC_000002.12 2 182541418 - PDE1A NNNTCTGCCAGGTTACTGAG NTG 2 0.0229 Tier II
25 NC_000002.12 2 167352798 - B3GALT1 NNNTCTTTCAGGTTACTGAG NGT 2 0.0141 Tier II
26 NC_000014.9 14 74681325 - AREL1 NNNTCATCCAGGTTACTGAG NGT 2 0.014 Tier II
27 NC_000020.11 20 58392624 + VAPB NNNTCTTCCAGGTTACTCAG NGT 2 0.0077 Tier II
28 NC_000017.11 17 34891147 - LOC105371742 NNNTCCTCCAGGTTGCTGAG NGG 2 0.5909 Tier III
29 NC_000007.14 7 116681160 - COMETT NNNTCTTCCTGGTAACTGAG NGG 2 0.5462 Tier III
30 NC_000017.11 17 73790569 + LINC00469 NNNCTTTCCAGGTTACTGAG NGG 2 0.4118 Tier III
31 NC_000017.11 17 73790569 + LINC02092 NNNCTTTCCAGGTTACTGAG NGG 2 0.4118 Tier III
32 NC_000024.10 Y 9806303 + TTTY22 NNNTCTTCCAGGTTACTCAT NGG 2 0.3333 Tier III
33 NC_000005.10 5 162623930 - LOC105377698 NNNTCTTCCTGGTTATTGAG NGG 2 0.2715 Tier III
34 NC_000011.10 11 92655031 + LOC124902732 NNNTCTTCCAGGTTTCTGAA NGG 2 0.1875 Tier III
35 NC_000013.11 13 46096982 - CPB2-AS1 NNNTCTTCCAGGTTCTTGAG NGG 2 0.0839 Tier III
36 NC_000015.10 15 46739877 - LOC729316 NNNTCTTCCAGGTTATTGAG NAG 2 0.0798 Tier III
37 NC_000005.10 5 70098830 + NAIPP2 NNNTCTTCCAGGTTACTAAG NGA 2 0.0481 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 78446990 - Pdxk NNNTGTTCCAGGTTGCTGAG NGG 2 0.39 Tier I
2 NC_000067.6 1 167846648 - Lmx1a NNNTCTCCCAGGTTACTGAG NGA 2 0.0477 Tier I
3 NC_000071.6 5 23975997 + Fam126a NNNTCTTCTAGGTTACAGAG NGG 2 0.4667 Tier II
4 NC_000069.6 3 114160199 - Col11a1 NNNTCTTCCAGGATGCTGAG NGG 2 0.45 Tier II
5 NC_000083.6 17 47374194 + Mrps10 NNNTCTTCCGGGTTACTAAG NGG 2 0.3846 Tier II
6 NC_000068.7 2 52152051 - Neb NNNTCTCCCAGGTTACTCAG NGG 2 0.3274 Tier II
7 NC_000077.6 11 60236628 + Tom1l2 NNNTCTGCCAGGTTACAGAG NGG 2 0.3137 Tier II
8 NC_000072.6 6 129379687 + Clec12b NNNTCTTCCAGGTTACAGTG NGG 2 0.2872 Tier II
9 NC_000079.6 13 73559951 + Slc6a3 NNNTCTACCAGGTTTCTGAG NGG 2 0.175 Tier II
10 NC_000077.6 11 5109030 - Emid1 NNNTCTTCAAGGTTTCTGAG NGG 2 0.1714 Tier II
11 NC_000068.7 2 103667280 + Abtb2 NNNTCTTCCAGGGTACTCAG NGG 2 0.1242 Tier II
12 NC_000068.7 2 83806726 - Itgav NNNTCTTCCAGGTTACTTAG NGA 2 0.0463 Tier II
13 NC_000080.6 14 72688865 + Fndc3a NNNCCTTCCAGGTTACTGAG NGA 2 0.0449 Tier II
14 NC_000076.6 10 102203764 + Mgat4c NNNTCTTTCAGGTTACTGAG NTG 2 0.0341 Tier II
15 NC_000068.7 2 162993139 + Sgk2 NNNTCTTCCAGGTTACTGTG NGT 2 0.0087 Tier II
16 NC_000082.6 16 45814592 - Phldb2 NNNTCTTCCAGGTTACTGTG NGT 2 0.0087 Tier II
17 NC_000068.7 2 34942066 - Traf1 NNNTCTTCCAGGTGACTGAG NGA 2 0.0 Tier II
18 NC_000076.6 10 121951669 - Srgap1 NNNTCTTCCTGGTGACTGAG NGG 2 0.0 Tier II
19 NC_000077.6 11 72363949 + Gm34317 NNNTCTGCCAAGTTACTGAG NGG 2 0.5882 Tier III
20 NC_000067.6 1 62841920 + Gm39652 NNNTCTTCCAGGTTACTCAC NGG 2 0.2041 Tier III
21 NC_000082.6 16 50665512 - Gm9575 NNNTCTTCCAGGTTGCTGAG NAG 2 0.1685 Tier III
22 NC_000069.6 3 60786889 + Gm553061 NNNTCTTCCAGGTTACTGAG NAT 2 0.0 Tier III
23 NC_000078.6 12 115052892 + Igh NNNTCTTCCTGGTGACTGAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)