Construct: sgRNA BRDN0001147617
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGACAATCTACTCACAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP3 (4356)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77812
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
43818046 |
+ |
MPP3 |
NNNGACAATCTACTCACAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
79037361 |
+ |
RASGRF1 |
NNNGACAATCTTCTCACAGA |
NGG |
2 |
0.3125 |
Tier II |
| 3 |
NC_000011.10 |
11 |
47541226 |
+ |
CELF1 |
NNNGACAATCTACTCACAGT |
NGA |
2 |
0.0486 |
Tier II |
| 4 |
NC_000002.12 |
2 |
153387018 |
+ |
GALNT13 |
NNNGACAATCTACTCACAGA |
NGT |
2 |
0.0151 |
Tier II |
| 5 |
NC_000017.11 |
17 |
37687207 |
- |
HNF1B |
NNNGACAATCCACTCTCAGG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000003.12 |
3 |
76808983 |
- |
ROBO2 |
NNNGACGATCTACTCTCAGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000002.12 |
2 |
57886176 |
+ |
LOC124907769 |
NNNGACAATCTACTCACAGC |
NGG |
1 |
0.4286 |
Tier III |
| 8 |
NC_000014.9 |
14 |
53615652 |
- |
LOC105370504 |
NNNGACAATCTAATCACAGG |
NGG |
1 |
0.3846 |
Tier III |
| 9 |
NC_000015.10 |
15 |
96700051 |
+ |
LOC105371002 |
NNNGACAATCCCCTCACAGG |
NGG |
2 |
0.1754 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
130817917 |
+ |
Caln1 |
NNNGACAACTTACTCACAGG |
NGG |
2 |
0.8688 |
Tier II |
| 2 |
NC_000081.6 |
15 |
53825562 |
+ |
Samd12 |
NNNAACAAACTACTCACAGG |
NGG |
2 |
0.8357 |
Tier II |
| 3 |
NC_000082.6 |
16 |
44964245 |
- |
Cd200r3 |
NNNAACAAACTACTCACAGG |
NGG |
2 |
0.8357 |
Tier II |
| 4 |
NC_000067.6 |
1 |
82633669 |
+ |
Col4a3 |
NNNGACAAAATACTCACAGG |
NGG |
2 |
0.8048 |
Tier II |
| 5 |
NC_000074.6 |
8 |
54931551 |
- |
Gpm6a |
NNNGACAAAATACTCACAGG |
NGG |
2 |
0.8048 |
Tier II |
| 6 |
NC_000086.7 |
X |
84642281 |
+ |
Dmd |
NNNGACAAAATACTCACAGG |
NGG |
2 |
0.8048 |
Tier II |
| 7 |
NC_000074.6 |
8 |
58619757 |
+ |
Galntl6 |
NNNAACAATATACTCACAGG |
NGG |
2 |
0.78 |
Tier II |
| 8 |
NC_000086.7 |
X |
87604515 |
- |
Il1rapl1 |
NNNAACAATATACTCACAGG |
NGG |
2 |
0.78 |
Tier II |
| 9 |
NC_000082.6 |
16 |
14445539 |
- |
Abcc1 |
NNNGACAATCCACTCACAGG |
NGG |
1 |
0.6667 |
Tier II |
| 10 |
NC_000071.6 |
5 |
66818703 |
- |
Limch1 |
NNNGAGAATCTACTCACAGA |
NGG |
2 |
0.4688 |
Tier II |
| 11 |
NC_000079.6 |
13 |
117629543 |
- |
Hcn1 |
NNNGACAATATACTCATAGG |
NGG |
2 |
0.4044 |
Tier II |
| 12 |
NC_000069.6 |
3 |
158517460 |
- |
Lrrc7 |
NNNGACAATCTACTCACAGA |
NAG |
2 |
0.2431 |
Tier II |
| 13 |
NC_000077.6 |
11 |
106069011 |
- |
Taco1 |
NNNTACAATCTACTCATAGG |
NGG |
2 |
0.1697 |
Tier II |
| 14 |
NC_000068.7 |
2 |
72210694 |
- |
Rapgef4 |
NNNGACAATCAAGTCACAGG |
NGG |
2 |
0.1023 |
Tier II |
| 15 |
NC_000074.6 |
8 |
53588203 |
- |
Neil3 |
NNNGACAATCTAGTCACAGG |
NGA |
2 |
0.0095 |
Tier II |
| 16 |
NC_000072.6 |
6 |
83698475 |
+ |
Vax2os |
NNNGACAAAATACTCACAGG |
NGG |
2 |
0.8048 |
Tier III |
| 17 |
NC_000076.6 |
10 |
124491623 |
+ |
4930503E24Rik |
NNNAACAATATACTCACAGG |
NGG |
2 |
0.78 |
Tier III |
| 18 |
NC_000083.6 |
17 |
66522368 |
- |
Gm41608 |
NNNGACTATCAACTCACAGG |
NGG |
2 |
0.3281 |
Tier III |
Other clones with same target sequence:
(none)
