Construct: sgRNA BRDN0001147619
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCAAACATGACCCCACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD2 (25865)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77615
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
46713941 |
- |
PRKD2 |
NNNCAAACATGACCCCACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
46091159 |
+ |
KANSL1 |
NNNCAAACCTGACCCCATGT |
NGG |
2 |
0.3673 |
Tier II |
| 3 |
NC_000001.11 |
1 |
75346112 |
+ |
SLC44A5 |
NNNGAAACATGACCCCACAT |
NGG |
2 |
0.3571 |
Tier II |
| 4 |
NC_000012.12 |
12 |
12944447 |
+ |
GPRC5D |
NNNCAAACATGACACCACCT |
NGG |
2 |
0.1569 |
Tier II |
| 5 |
NC_000019.10 |
19 |
1472937 |
+ |
APC2 |
NNNCCAACATGACCACACGT |
NGG |
2 |
0.1111 |
Tier II |
| 6 |
NC_000016.10 |
16 |
88404854 |
- |
ZNF469 |
NNNCCAACATGACCCCAGGT |
NGG |
2 |
0.0667 |
Tier II |
| 7 |
NC_000023.11 |
X |
27684227 |
+ |
DCAF8L2 |
NNNCAAACATGACCACAGGT |
NGG |
2 |
0.0296 |
Tier II |
| 8 |
NC_000002.12 |
2 |
209517737 |
+ |
MAP2 |
NNNCTAACATGACCCCACGT |
NGA |
2 |
0.0253 |
Tier II |
| 9 |
NC_000012.12 |
12 |
12944447 |
+ |
GPRC5D-AS1 |
NNNCAAACATGACACCACCT |
NGG |
2 |
0.1569 |
Tier III |
| 10 |
NC_000014.9 |
14 |
106440026 |
- |
IGH |
NNNCAAACATGTCCCCACGT |
NGA |
2 |
0.0231 |
Tier III |
| 11 |
NC_000014.9 |
14 |
106440026 |
- |
LOC124903400 |
NNNCAAACATGTCCCCACGT |
NGA |
2 |
0.0231 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6233922 |
+ |
Ppp2r5b |
NNNCAAACATCACCCCACAT |
NGG |
2 |
0.3061 |
Tier I |
| 2 |
NC_000069.6 |
3 |
103625768 |
+ |
Syt6 |
NNNCAAATATGACCCCACTT |
NGG |
2 |
0.5833 |
Tier II |
| 3 |
NC_000080.6 |
14 |
45281443 |
+ |
Gpr137c |
NNNTAAACATGACCCCACAT |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000083.6 |
17 |
13855592 |
+ |
Afdn |
NNNCAGACATGACCCCATGT |
NGG |
2 |
0.4592 |
Tier II |
Other clones with same target sequence:
(none)
