Construct: sgRNA BRDN0001147622
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGAGTCATCTCCAATGTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DLG1 (1739)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76620
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
197140215 |
+ |
DLG1 |
NNNAGTCATCTCCAATGTGT |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000002.12 |
2 |
162063125 |
- |
DPP4 |
NNNAGTCACATCCAATGTGT |
NGG |
2 |
0.8 |
Tier II |
3 |
NC_000005.10 |
5 |
109192414 |
+ |
FER |
NNNAGTCATCTCCAGTGTGT |
NGG |
1 |
0.65 |
Tier II |
4 |
NC_000005.10 |
5 |
153523744 |
- |
GRIA1 |
NNNAGTCATCTCCTATATGT |
NGG |
2 |
0.4978 |
Tier II |
5 |
NC_000007.14 |
7 |
141380175 |
- |
TMEM178B |
NNNAGTCAGCACCAATGTGT |
NGG |
2 |
0.4643 |
Tier II |
6 |
NC_000022.11 |
22 |
21829312 |
- |
MAPK1 |
NNNAGTCTCCTCCAATGTGT |
NGG |
2 |
0.3956 |
Tier II |
7 |
NC_000016.10 |
16 |
7051632 |
- |
RBFOX1 |
NNNAGTCTTCTCCAATGTTT |
NGG |
2 |
0.2857 |
Tier II |
8 |
NC_000020.11 |
20 |
53013691 |
- |
TSHZ2 |
NNNAGTCATCACCACTGTGT |
NGG |
2 |
0.2045 |
Tier II |
9 |
NC_000003.12 |
3 |
125462210 |
+ |
SNX4 |
NNNAGTCATATCCCATGTGT |
NGG |
2 |
0.1857 |
Tier II |
10 |
NC_000005.10 |
5 |
95545582 |
- |
SKIC3 |
NNNAGTCATCTGCAATCTGT |
NGG |
2 |
0.1046 |
Tier II |
11 |
NC_000010.11 |
10 |
82749897 |
- |
NRG3 |
NNNAGTAATCTCCAATGTGC |
NGG |
2 |
0.0682 |
Tier II |
12 |
NC_000016.10 |
16 |
11084697 |
+ |
CLEC16A |
NNNAGTCATCTCCACTGTGG |
NGG |
2 |
0.0481 |
Tier II |
13 |
NC_000013.11 |
13 |
33183701 |
+ |
STARD13 |
NNNAGTCATCTCCAATCTGG |
NGG |
2 |
0.0415 |
Tier II |
14 |
NC_000016.10 |
16 |
7051632 |
- |
LOC105371068 |
NNNAGTCTTCTCCAATGTTT |
NGG |
2 |
0.2857 |
Tier III |
15 |
NC_000019.10 |
19 |
22621681 |
- |
LINC01785 |
NNNATTCATCTCCAAAGTGT |
NGG |
2 |
0.2727 |
Tier III |
16 |
NC_000008.11 |
8 |
116839545 |
+ |
LOC112268030 |
NNNAGTCATCCCAAATGTGT |
NGG |
2 |
0.2564 |
Tier III |
17 |
NC_000004.12 |
4 |
103980199 |
- |
LINC02503 |
NNNAGTCATCTACATTGTGT |
NGG |
2 |
0.1429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000082.6 |
16 |
31790227 |
- |
Dlg1 |
NNNAGTCATCTCCAATGTGT |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000069.6 |
3 |
127870966 |
+ |
Fam241a |
NNNAGTCATCTCCAATGGGT |
NCG |
2 |
0.0357 |
Tier I |
3 |
NC_000071.6 |
5 |
102752461 |
- |
Arhgap24 |
NNNAGTCATTTACAATGTGT |
NGG |
2 |
0.6723 |
Tier II |
4 |
NC_000072.6 |
6 |
141273389 |
+ |
Pde3a |
NNNAGTCATCACCAATGGGT |
NGG |
2 |
0.25 |
Tier II |
5 |
NC_000080.6 |
14 |
48473011 |
+ |
Tmem260 |
NNNAGTCATCTGCAATGTGA |
NGG |
2 |
0.25 |
Tier II |
6 |
NC_000067.6 |
1 |
181392409 |
+ |
Cnih3 |
NNNAGTCATCTCCCATATGT |
NGG |
2 |
0.2 |
Tier II |
7 |
NC_000079.6 |
13 |
8555400 |
+ |
Adarb2 |
NNNACTCATCTCCAATGTGT |
NTG |
2 |
0.0306 |
Tier II |
8 |
NC_000067.6 |
1 |
36589869 |
+ |
Fam178b |
NNNAGTCATCTCCAATGTGA |
NTG |
2 |
0.0219 |
Tier II |
9 |
NC_000080.6 |
14 |
20681166 |
- |
Sec24c |
NNNAATCATCTCCAATGTGT |
NGC |
2 |
0.0193 |
Tier II |
10 |
NC_000072.6 |
6 |
144471939 |
- |
Sox5 |
NNNAGTCATCTCCAATGTGG |
NGA |
2 |
0.0123 |
Tier II |
11 |
NC_000078.6 |
12 |
17716628 |
+ |
Hpcal1 |
NNNAGTCATCTCCATTGTGT |
NTG |
2 |
0.0078 |
Tier II |
12 |
NC_000075.6 |
9 |
79508552 |
- |
Gm10635 |
NNNCGTCATCTCAAATGTGT |
NGG |
2 |
0.1357 |
Tier III |
13 |
NC_000084.6 |
18 |
68928623 |
+ |
4930546C10Rik |
NNNAGTCATTTCCAATGTGT |
NTG |
2 |
0.0367 |
Tier III |
14 |
NC_000078.6 |
12 |
17716628 |
+ |
Gm40842 |
NNNAGTCATCTCCATTGTGT |
NTG |
2 |
0.0078 |
Tier III |
Other clones with same target sequence:
(none)