Construct: sgRNA BRDN0001147622

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGAGTCATCTCCAATGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG1 (1739)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76620

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 197140215 + DLG1 NNNAGTCATCTCCAATGTGT NGG 0 1.0 Tier I
2 NC_000002.12 2 162063125 - DPP4 NNNAGTCACATCCAATGTGT NGG 2 0.8 Tier II
3 NC_000005.10 5 109192414 + FER NNNAGTCATCTCCAGTGTGT NGG 1 0.65 Tier II
4 NC_000005.10 5 153523744 - GRIA1 NNNAGTCATCTCCTATATGT NGG 2 0.4978 Tier II
5 NC_000007.14 7 141380175 - TMEM178B NNNAGTCAGCACCAATGTGT NGG 2 0.4643 Tier II
6 NC_000022.11 22 21829312 - MAPK1 NNNAGTCTCCTCCAATGTGT NGG 2 0.3956 Tier II
7 NC_000016.10 16 7051632 - RBFOX1 NNNAGTCTTCTCCAATGTTT NGG 2 0.2857 Tier II
8 NC_000020.11 20 53013691 - TSHZ2 NNNAGTCATCACCACTGTGT NGG 2 0.2045 Tier II
9 NC_000003.12 3 125462210 + SNX4 NNNAGTCATATCCCATGTGT NGG 2 0.1857 Tier II
10 NC_000005.10 5 95545582 - SKIC3 NNNAGTCATCTGCAATCTGT NGG 2 0.1046 Tier II
11 NC_000010.11 10 82749897 - NRG3 NNNAGTAATCTCCAATGTGC NGG 2 0.0682 Tier II
12 NC_000016.10 16 11084697 + CLEC16A NNNAGTCATCTCCACTGTGG NGG 2 0.0481 Tier II
13 NC_000013.11 13 33183701 + STARD13 NNNAGTCATCTCCAATCTGG NGG 2 0.0415 Tier II
14 NC_000016.10 16 7051632 - LOC105371068 NNNAGTCTTCTCCAATGTTT NGG 2 0.2857 Tier III
15 NC_000019.10 19 22621681 - LINC01785 NNNATTCATCTCCAAAGTGT NGG 2 0.2727 Tier III
16 NC_000008.11 8 116839545 + LOC112268030 NNNAGTCATCCCAAATGTGT NGG 2 0.2564 Tier III
17 NC_000004.12 4 103980199 - LINC02503 NNNAGTCATCTACATTGTGT NGG 2 0.1429 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 31790227 - Dlg1 NNNAGTCATCTCCAATGTGT NGG 0 1.0 Tier I
2 NC_000069.6 3 127870966 + Fam241a NNNAGTCATCTCCAATGGGT NCG 2 0.0357 Tier I
3 NC_000071.6 5 102752461 - Arhgap24 NNNAGTCATTTACAATGTGT NGG 2 0.6723 Tier II
4 NC_000072.6 6 141273389 + Pde3a NNNAGTCATCACCAATGGGT NGG 2 0.25 Tier II
5 NC_000080.6 14 48473011 + Tmem260 NNNAGTCATCTGCAATGTGA NGG 2 0.25 Tier II
6 NC_000067.6 1 181392409 + Cnih3 NNNAGTCATCTCCCATATGT NGG 2 0.2 Tier II
7 NC_000079.6 13 8555400 + Adarb2 NNNACTCATCTCCAATGTGT NTG 2 0.0306 Tier II
8 NC_000067.6 1 36589869 + Fam178b NNNAGTCATCTCCAATGTGA NTG 2 0.0219 Tier II
9 NC_000080.6 14 20681166 - Sec24c NNNAATCATCTCCAATGTGT NGC 2 0.0193 Tier II
10 NC_000072.6 6 144471939 - Sox5 NNNAGTCATCTCCAATGTGG NGA 2 0.0123 Tier II
11 NC_000078.6 12 17716628 + Hpcal1 NNNAGTCATCTCCATTGTGT NTG 2 0.0078 Tier II
12 NC_000075.6 9 79508552 - Gm10635 NNNCGTCATCTCAAATGTGT NGG 2 0.1357 Tier III
13 NC_000084.6 18 68928623 + 4930546C10Rik NNNAGTCATTTCCAATGTGT NTG 2 0.0367 Tier III
14 NC_000078.6 12 17716628 + Gm40842 NNNAGTCATCTCCATTGTGT NTG 2 0.0078 Tier III
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Other clones with same target sequence:

(none)