Construct: sgRNA BRDN0001147624

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCACTGGTCACAAAAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5K1B (8395)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77083

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 68889042 - PIP5K1B NNNACTGGTCACAAAAAACA NGG 0 1.0 Tier I
2 NC_000012.12 12 42121866 + GXYLT1 NNNACTGACCACAAAAAACA NGG 2 0.9231 Tier II
3 NC_000012.12 12 80217956 - OTOGL NNNATTGGTAACAAAAAACA NGG 2 0.5515 Tier II
4 NC_000009.12 9 113050594 - ZFP37 NNNACTGGTTACAAAAATCA NGG 2 0.4706 Tier II
5 NC_000004.12 4 161932126 + FSTL5 NNNACTGGACACAAAAAATA NGG 2 0.4286 Tier II
6 NC_000018.10 18 36737035 + FHOD3 NNNTCTGCTCACAAAAAACA NGG 2 0.3916 Tier II
7 NC_000013.11 13 59996093 + DIAPH3 NNNACTGGTCAAATAAAACA NGG 2 0.381 Tier II
8 NC_000012.12 12 103545279 - C12orf42 NNNACTGTTCACAAAAAACT NGG 2 0.375 Tier II
9 NC_000020.11 20 13602288 + TASP1 NNNTATGGTCACAAAAAACA NGG 2 0.3636 Tier II
10 NC_000012.12 12 121256084 + CAMKK2 NNNACTGGTCAAAGAAAACA NGG 2 0.3333 Tier II
11 NC_000011.10 11 40283091 - LRRC4C NNNGCTGGTCACAAAAATCA NGG 2 0.3125 Tier II
12 NC_000005.10 5 39161642 - FYB1 NNNACTGGTCACATAAAATA NGG 2 0.2462 Tier II
13 NC_000015.10 15 83587338 - SH3GL3 NNNACGGGTCACAAAAAAAA NGG 2 0.2449 Tier II
14 NC_000006.12 6 68930791 - ADGRB3 NNNACTGGTGACAAAAAAAA NGG 2 0.1667 Tier II
15 NC_000002.12 2 176325581 + MTX2 NNNACTGGTCATTAAAAACA NGG 2 0.1615 Tier II
16 NC_000020.11 20 8839639 + PLCB1 NNNACTGGTCACAAAAATCA NAG 2 0.1296 Tier II
17 NC_000012.12 12 55684438 + TMT1B NNNACTGGTCACAAAAGTCA NGG 2 0.0882 Tier II
18 NC_000007.14 7 78359416 - MAGI2 NNNTCTGGTCACAAAATACA NGG 2 0.0848 Tier II
19 NC_000002.12 2 182708990 + PDE1A NNNACTGGTCAAAAAAAACA NGA 2 0.0496 Tier II
20 NC_000007.14 7 24732042 - GSDME NNNACTGGTCACAATAAACC NGG 2 0.0455 Tier II
21 NC_000005.10 5 54219671 + ARL15 NNNACTGGTCACAAAAAAGA NAG 2 0.0324 Tier II
22 NC_000003.12 3 134607654 - CEP63 NNNACTGGTCACAAAAAAGC NGG 2 0.0284 Tier II
23 NC_000003.12 3 134607654 - KY NNNACTGGTCACAAAAAAGC NGG 2 0.0284 Tier II
24 NC_000016.10 16 66623658 + CMTM4 NNNACTGGTCACACAAAAGA NGG 2 0.0268 Tier II
25 NC_000016.10 16 6194273 - RBFOX1 NNNACTGGTCACAAAGAACA NTG 2 0.0075 Tier II
26 NC_000017.11 17 45477642 - PLEKHM1 NNNACTGGTCACAAAAAGCA NGT 2 0.0065 Tier II
27 NC_000006.12 6 119824912 + LOC105377975 NNNACAGGTCACAAAAAACT NGG 2 0.52 Tier III
28 NC_000002.12 2 163313488 + LOC105373727 NNNACTGGTCACAAAAATCA NGG 1 0.5 Tier III
29 NC_000004.12 4 138876025 + LOC105377448 NNNACTGGCCACATAAAACA NGG 2 0.4923 Tier III
30 NC_000024.10 Y 21298378 + LOC107987347 NNNACTGCTCACAAGAAACA NGG 2 0.4 Tier III
31 NC_000023.11 X 28042583 + LOC105373151 NNNTCTGGTCACAAAAAAAA NGG 2 0.2727 Tier III
32 NC_000009.12 9 90485898 - LINC01501 NNNACTGCTCACAAAAAACA NAG 2 0.1595 Tier III
33 NC_000003.12 3 6534798 - LOC105376944 NNNTCTGGTCACACAAAACA NGG 2 0.1364 Tier III
34 NC_000004.12 4 147002326 + LOC105377475 NNNACTTGTCACAAAACACA NGG 2 0.1008 Tier III
35 NC_000001.11 1 193868634 + LOC124904475 NNNACTGGTCACATAACACA NGG 2 0.0941 Tier III
36 NC_000007.14 7 78359416 - RPL13AP17 NNNTCTGGTCACAAAATACA NGG 2 0.0848 Tier III
37 NC_000002.12 2 182708990 + LOC101929976 NNNACTGGTCAAAAAAAACA NGA 2 0.0496 Tier III
38 NC_000017.11 17 64824367 - PLEKHM1P1 NNNACTGGTCACAAAAAACA NGT 1 0.0161 Tier III
39 NC_000020.11 20 6451722 - CASC20 NNNACTGGTCCCAAAAAACA NGT 2 0.0065 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 146977942 - Ppfibp1 NNNAATGATCACAAAAAACA NGG 2 0.5714 Tier II
2 NC_000068.7 2 173721560 - 1700010B08Rik NNNACTGGACAGAAAAAACA NGG 2 0.4127 Tier II
3 NC_000072.6 6 92513682 - Prickle2 NNNACAGGTCACAGAAAACA NGG 2 0.4044 Tier II
4 NC_000067.6 1 62575533 + Pard3b NNNACTGGTCAAATAAAACA NGG 2 0.381 Tier II
5 NC_000073.6 7 115898782 - Sox6 NNNAATGGTCACAAAAATCA NGG 2 0.2857 Tier II
6 NC_000070.6 4 65169797 - Pappa NNNACTGGTCATAAAAAATA NGG 2 0.2485 Tier II
7 NC_000079.6 13 17408831 + Sugct NNNAGTGGTCCCAAAAAACA NGG 2 0.24 Tier II
8 NC_000074.6 8 56060514 - Glra3 NNNAATGGTCCCAAAAAACA NGG 2 0.2286 Tier II
9 NC_000080.6 14 56446596 - Rnf17 NNNAATGGTCCCAAAAAACA NGG 2 0.2286 Tier II
10 NC_000083.6 17 78400112 + Fez2 NNNACTGGTCACAGAAAAAA NGG 2 0.2 Tier II
11 NC_000067.6 1 60201918 - Nbeal1 NNNACTTGTCTCAAAAAACA NGG 2 0.1758 Tier II
12 NC_000075.6 9 53371413 - Exph5 NNNCCTGGTCACAAAAAATA NGG 2 0.1629 Tier II
13 NC_000067.6 1 136099768 + Cacna1s NNNAGTGGTCACAAAAACCA NGG 2 0.1143 Tier II
14 NC_000074.6 8 48264409 + Tenm3 NNNCCTGGTCTCAAAAAACA NGG 2 0.1086 Tier II
15 NC_000080.6 14 44445932 + Gm3371 NNNACTGGTCAAAAAATACA NGG 2 0.0952 Tier II
16 NC_000071.6 5 65013277 - Fam114a1 NNNACTGGTCCCAAAAAACC NGG 2 0.0909 Tier II
17 NC_000081.6 15 95385653 + Nell2 NNNATTGGTCACAAAATACA NGG 2 0.0848 Tier II
18 NC_000083.6 17 33813514 + Kank3 NNNACTGGTCACTACAAACA NGG 2 0.0818 Tier II
19 NC_000068.7 2 32648663 + Eng NNNACTGGGCACAAAAAACA NGC 2 0.0138 Tier II
20 NC_000069.6 3 112055401 - Gm6602 NNNACTGGTTACATAAAACA NGG 2 0.502 Tier III
21 NC_000083.6 17 78400112 + Gm32336 NNNACTGGTCACAGAAAAAA NGG 2 0.2 Tier III
22 NC_000082.6 16 5359101 - Gm41414 NNNACTGGTCTCAAAAAATA NGG 2 0.142 Tier III
23 NC_000071.6 5 65013277 - Gm32374 NNNACTGGTCCCAAAAAACC NGG 2 0.0909 Tier III
24 NC_000067.6 1 51515655 + Gm17767 NNNACTGGTCACACAAAGCA NGG 2 0.0857 Tier III
25 NC_000069.6 3 99238096 + Gm38468 NNNACTGGTCAGAAAAACCA NGG 2 0.0847 Tier III
26 NC_000080.6 14 59054332 + Gm41162 NNNACTGGTCACACAAAACC NGG 2 0.0487 Tier III
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Other clones with same target sequence:

(none)