Construct: sgRNA BRDN0001147626

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGCGGAGCCCAGTACCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL5 (6792)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77196

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 18604464 + CDKL5 NNNCGGAGCCCAGTACCAGT NGG 0 1.0 Tier I
2 NC_000007.14 7 44139443 - MYL7 NNNCAGAGCCCAGTACCAGT NAG 2 0.2247 Tier I
3 NC_000014.9 14 102593299 + RCOR1 NNNCGGACCCCAGTACCAGG NGG 2 0.1086 Tier I
4 NC_000001.11 1 197429591 + CRB1 NNNCGGAGCCCAGTGCCAGC NGG 2 0.0591 Tier I
5 NC_000014.9 14 89302740 + FOXN3 NNNCAGAGCCCAGTACCAAT NGG 2 0.619 Tier II
6 NC_000003.12 3 134961126 - EPHB1 NNNCAGGGCCCAGTACCAGT NGG 2 0.6118 Tier II
7 NC_000010.11 10 116158788 + GFRA1 NNNTGGAGCCCAGTACCAGA NGG 2 0.45 Tier II
8 NC_000002.12 2 17643206 + VSNL1 NNNTGGAGCCCAGCACCAGT NGG 2 0.2286 Tier II
9 NC_000012.12 12 49646640 + FMNL3 NNNCGGTGCCCAGTACCTGT NGG 2 0.2188 Tier II
10 NC_000009.12 9 115212005 + DELEC1 NNNGGGAGCACAGTACCAGT NGG 2 0.4333 Tier III
11 NC_000002.12 2 17643206 + LOC124908054 NNNTGGAGCCCAGCACCAGT NGG 2 0.2286 Tier III
12 NC_000002.12 2 10432571 - LOC124907732 NNNCGGAGCCCAGGACCAGC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 56621236 + Lingo1 NNNCTGAGCCCAGTACCAGC NGG 2 0.0273 Tier I
2 NC_000084.6 18 21849029 + Ccdc178 NNNCAGAGCTCAGTACCAGT NGG 2 0.8157 Tier II
3 NC_000072.6 6 88420652 - Eefsec NNNAGGAGCCAAGTACCAGT NGG 2 0.6316 Tier II
4 NC_000073.6 7 79392634 + Fanci NNNTGGAGCCCAGTGCCAGT NGG 2 0.52 Tier II
5 NC_000077.6 11 97837473 + Lasp1 NNNCAGAGCCCAGTACCAGA NGG 2 0.4875 Tier II
6 NC_000083.6 17 49545565 - Daam2 NNNCAGAGCCCTGTACCAGT NGG 2 0.2889 Tier II
7 NC_000069.6 3 116529682 - Dbt NNNTGGAGCCCAGTCCCAGT NGG 2 0.2182 Tier II
8 NC_000082.6 16 4375982 - Adcy9 NNNTGGAGCCCAGTACCAGT NAG 2 0.2074 Tier II
9 NC_000071.6 5 144948241 + Smurf1 NNNCTGTGCCCAGTACCAGT NGG 2 0.1313 Tier II
10 NC_000086.7 X 138271226 + Il1rapl2 NNNGGGAGCCCAGTTCCAGT NGG 2 0.1 Tier II
11 NC_000071.6 5 118153484 + Fbxw8 NNNTGGAGCCCAGGACCAGT NGG 2 0.0 Tier II
12 NC_000080.6 14 57107010 + Gm32912 NNNAGGAGCTCAGTACCAGT NGG 2 0.7926 Tier III
13 NC_000084.6 18 68574463 + Gm30700 NNNTGGAGCCCAGTACCAAT NGG 2 0.5714 Tier III
14 NC_000068.7 2 79122924 - Gm35748 NNNCAGAGCCCAGTCCCAGT NGG 2 0.2364 Tier III
15 NC_000067.6 1 73807467 - 6030407O03Rik NNNGGGAGCCCAGTCCCAGT NGG 2 0.1364 Tier III
16 NC_000067.6 1 73807467 - Gm29183 NNNGGGAGCCCAGTCCCAGT NGG 2 0.1364 Tier III
17 NC_000084.6 18 74977981 + 9030625G05Rik NNNCGGAGACCAGTAGCAGT NGG 2 0.1319 Tier III
18 NC_000068.7 2 75284668 + 2600014E21Rik NNNCGGAGCCCAGTTCCAGT NGA 2 0.0139 Tier III
19 NC_000068.7 2 75284668 + Gm34401 NNNCGGAGCCCAGTTCCAGT NGA 2 0.0139 Tier III
20 NC_000070.6 4 152769529 - Gm30851 NNNCGGAGCCCTGGACCAGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)