Construct: sgRNA BRDN0001147629

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGACGAGTTTGAGAACATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK4 (10298)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77573

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 39172988 + PAK4 NNNCGAGTTTGAGAACATGT NGG 0 1.0 Tier I
2 NC_000007.14 7 43555248 + HECW1 NNNCAAGTTTTAGAACATGT NGG 2 0.3333 Tier II
3 NC_000001.11 1 183038726 - LAMC1 NNNGGATTTTGAGAACATGT NGG 2 0.2857 Tier II
4 NC_000010.11 10 87547326 - MINPP1 NNNAGAGTTTGAGAACAGGT NGG 2 0.2807 Tier II
5 NC_000001.11 1 24104761 - MYOM3 NNNGGAGTTTGAGAACATCT NGG 2 0.2241 Tier II
6 NC_000003.12 3 122053593 - ILDR1 NNNGGAGTTTGAGAACATCT NGG 2 0.2241 Tier II
7 NC_000002.12 2 85056980 + KCMF1 NNNCGAGTTTGAGACCATTT NGG 2 0.1818 Tier II
8 NC_000013.11 13 49324117 + CAB39L NNNGGAGTTTGAGAACAGGT NGG 2 0.1667 Tier II
9 NC_000003.12 3 77627498 - ROBO2 NNNCGAGTTTGAGAACAGCT NGG 2 0.1494 Tier II
10 NC_000001.11 1 62141774 + PATJ NNNCGAGTTTGAGAACAGGC NGG 2 0.0303 Tier II
11 NC_000010.11 10 57376742 + LOC105378313 NNNCCAGATTGAGAACATGT NGG 2 0.6286 Tier III
12 NC_000007.14 7 23229607 + LOC101927890 NNNGGAGTTTGAGACCATGT NGG 2 0.1364 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 28565202 - Pak4 NNNCGAATTTGAGAACATGT NGG 1 1.0 Tier I
2 NC_000071.6 5 107373362 - Brdt NNNTGAGTTTGAGAATATGT NGG 2 0.2462 Tier I
3 NC_000085.6 19 12036193 + Olfr1423 NNNCAAGTTTGAGAACCTGT NGG 2 0.1529 Tier I
4 NC_000085.6 19 12099090 + Olfr1427 NNNCGAGTTTGAGGACCTGT NGG 2 0.0824 Tier I
5 NC_000069.6 3 88294814 + Tsacc NNNCGAGTTTGAGGACATGC NGG 2 0.0424 Tier I
6 NC_000079.6 13 77767665 - Fam172a NNNTGAGTTTGAGAGCATGT NGG 2 0.52 Tier II
7 NC_000070.6 4 21991571 - Faxc NNNTGAGTGTGAGAACATGT NGG 2 0.4952 Tier II
8 NC_000070.6 4 58829769 - Ecpas NNNCAAGTTGGAGAACATGT NGG 2 0.4333 Tier II
9 NC_000084.6 18 23855132 + Mapre2 NNNAGAGTTTGAGAACATCT NGG 2 0.3775 Tier II
10 NC_000075.6 9 57460780 + Scamp5 NNNTGAGTTTGAGAACATCT NGG 2 0.3586 Tier II
11 NC_000082.6 16 90981141 - Synj1 NNNGGTGTTTGAGAACATGT NGG 2 0.3571 Tier II
12 NC_000081.6 15 66676344 + Tg NNNCCAGTTTTAGAACATGT NGG 2 0.3022 Tier II
13 NC_000078.6 12 91046552 + Cep128 NNNTGAGTTTGTGAACATGT NGG 2 0.2667 Tier II
14 NC_000074.6 8 119302502 + Cdh13 NNNCGAGTCTGAGACCATGT NGG 2 0.2517 Tier II
15 NC_000067.6 1 37906988 + Lyg2 NNNCGAGTTTGAGGACATCT NGG 2 0.2092 Tier II
16 NC_000073.6 7 79352979 + Abhd2 NNNGGAGTTTGAGAACAGGT NGG 2 0.1667 Tier II
17 NC_000081.6 15 23051260 - Cdh18 NNNCTAGTTTGAGAAGATGT NGG 2 0.0462 Tier II
18 NC_000071.6 5 144270605 - Baiap2l1 NNNTGAGTTTGAGAACATGT NGT 2 0.0129 Tier II
19 NC_000078.6 12 91046552 + 5430427M07Rik NNNTGAGTTTGTGAACATGT NGG 2 0.2667 Tier III
20 NC_000071.6 5 107373362 - Gm17202 NNNTGAGTTTGAGAATATGT NGG 2 0.2462 Tier III
21 NC_000073.6 7 79352979 + Gm24224 NNNGGAGTTTGAGAACAGGT NGG 2 0.1667 Tier III
22 NC_000080.6 14 24961786 + Gm32068 NNNAGAGTTTGAGAACATGT NTG 2 0.0328 Tier III
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Other clones with same target sequence:

(none)