Construct: sgRNA BRDN0001147634

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAGTTGTGTCCCCTGCATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2H1 (2965)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76601

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 18335809 - GTF2H1 NNNTTGTGTCCCCTGCATGT NGG 0 1.0 Tier I
2 NC_000014.9 14 104869587 + CEP170B NNNTTGTGTCCCCTGCAATT NGG 2 0.4444 Tier I
3 NC_000003.12 3 196640629 + NRROS NNNTCGTGGCCCCTGCATGT NGG 2 0.619 Tier II
4 NC_000010.11 10 113046262 + TCF7L2 NNNTTGTAGCCCCTGCATGT NGG 2 0.619 Tier II
5 NC_000015.10 15 39904616 + GPR176 NNNTTGTGTCCCCTGCAATT NGG 2 0.4444 Tier II
6 NC_000001.11 1 205809693 - SLC41A1 NNNTTTTGTCCCCTGCATTT NGG 2 0.4444 Tier II
7 NC_000007.14 7 746800 + DNAAF5 NNNCTCTGTCCCCTGCATGT NGG 2 0.4412 Tier II
8 NC_000007.14 7 69828411 - AUTS2 NNNTGCTGTCCCCTGCATGT NGG 2 0.4364 Tier II
9 NC_000011.10 11 128550059 - ETS1 NNNTTGTGTCCACTGCATGA NGG 2 0.4018 Tier II
10 NC_000001.11 1 48236308 - SLC5A9 NNNGTGGGTCCCCTGCATGT NGG 2 0.3676 Tier II
11 NC_000014.9 14 69131678 - DCAF5 NNNTTCTGTCCTCTGCATGT NGG 2 0.3671 Tier II
12 NC_000008.11 8 1258113 - DLGAP2 NNNTTGAGTCCCCTGTATGT NGG 2 0.2692 Tier II
13 NC_000012.12 12 526078 + B4GALNT3 NNNGTGTGTGCCCTGCATGT NGG 2 0.2431 Tier II
14 NC_000017.11 17 82012031 + ASPSCR1 NNNTGGTGTCCCCTGCAGGT NGG 2 0.2133 Tier II
15 NC_000009.12 9 3447702 + RFX3 NNNTTGTGTCTCCTGCATGA NGG 2 0.1731 Tier II
16 NC_000001.11 1 181053829 - MR1 NNNTTGTGTCCCCTACATGG NGG 2 0.1661 Tier II
17 NC_000010.11 10 1638893 - ADARB2 NNNTTGTGTCACCTGCATGG NGG 2 0.1324 Tier II
18 NC_000012.12 12 76521348 + OSBPL8 NNNTTGTGTGCCCTGCAGGT NGG 2 0.1296 Tier II
19 NC_000002.12 2 43280720 + THADA NNNTTGTGTCCCATGTATGT NGG 2 0.1183 Tier II
20 NC_000010.11 10 96456807 - TLL2 NNNTTGTGTCTCCTGCAGGT NGG 2 0.1026 Tier II
21 NC_000006.12 6 45882363 + CLIC5 NNNTTGTGTGCCCTGCCTGT NGG 2 0.0686 Tier II
22 NC_000011.10 11 78223157 - GAB2 NNNTTGTGCCCCCTGCATGT NGA 2 0.0641 Tier II
23 NC_000011.10 11 78223157 - USP35 NNNTTGTGCCCCCTGCATGT NGA 2 0.0641 Tier II
24 NC_000003.12 3 11810145 + TAMM41 NNNTTGTGTCTCCTGCCTGT NGG 2 0.0543 Tier II
25 NC_000002.12 2 191389897 + MYO1B NNNTTGTGTCCCTTGCATGT NGA 2 0.0486 Tier II
26 NC_000005.10 5 172174527 - STK10 NNNTTGTGTCCCCTGCATTT NGA 2 0.0463 Tier II
27 NC_000009.12 9 123579748 - DENND1A NNNTTGTGTCCCCTGCATTT NGC 2 0.0148 Tier II
28 NC_000014.9 14 103020279 - CDC42BPB NNNTTGTGTCCTCTGCATGT NGC 2 0.012 Tier II
29 NC_000012.12 12 54634505 - LACRT NNNTGGTGTCCCCTGCATGT NGT 2 0.0103 Tier II
30 NC_000004.12 4 161458063 - FSTL5 NNNTTGTGTCCCCTGCATGT NAC 2 0.0 Tier II
31 NC_000001.11 1 59023187 - LINC01358 NNNTTGTGCCCACTGCATGT NGG 2 0.6593 Tier III
32 NC_000016.10 16 49092675 + LOC105371241 NNNTTCTGTCCCCTGCAGGT NGG 2 0.2273 Tier III
33 NC_000009.12 9 123579748 - LOC105376266 NNNTTGTGTCCCCTGCATTT NGC 2 0.0148 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 83609281 - Tmc3 NNNTTGTGGGCCCTGCATGT NGG 2 0.2407 Tier I
2 NC_000073.6 7 46803872 - Gtf2h1 NNNTTGTGTCCCCTGCATGC NGG 1 0.0909 Tier I
3 NC_000075.6 9 28484557 - Opcml NNNTCCTGTCCCCTGCATGT NGG 2 0.6818 Tier II
4 NC_000071.6 5 139170455 - Dnaaf5 NNNTTGTGACCCCAGCATGT NGG 2 0.5748 Tier II
5 NC_000069.6 3 98148198 + Notch2 NNNTTCTCTCCCCTGCATGT NGG 2 0.4196 Tier II
6 NC_000074.6 8 115201260 + Wwox NNNTTTTTTCCCCTGCATGT NGG 2 0.4167 Tier II
7 NC_000080.6 14 20300939 + Nudt13 NNNCTGTGTCCCCAGCATGT NGG 2 0.4006 Tier II
8 NC_000067.6 1 136426237 + Ddx59 NNNTTCTGTCCTCTGCATGT NGG 2 0.3671 Tier II
9 NC_000074.6 8 16423024 - Csmd1 NNNTTGTGTCTCCTGCATGT NGG 1 0.3077 Tier II
10 NC_000070.6 4 32909579 - Ankrd6 NNNTTCTGTCCCCTGCATCT NGG 2 0.3056 Tier II
11 NC_000072.6 6 96117272 - Tafa1 NNNTTGTGTTCCCTCCATGT NGG 2 0.2567 Tier II
12 NC_000082.6 16 96047818 + Brwd1 NNNTTGTGACCCCTCCATGT NGG 2 0.2532 Tier II
13 NC_000076.6 10 81470285 - Celf5 NNNTTGTGTCCCCTGCAGAT NGG 2 0.2381 Tier II
14 NC_000076.6 10 70128676 + Ccdc6 NNNTTGTGTCCCCTGCAGGA NGG 2 0.1875 Tier II
15 NC_000076.6 10 64502336 - Ctnna3 NNNTTGTTTCCCCTGCATGT NAG 2 0.162 Tier II
16 NC_000079.6 13 117244342 - Emb NNNTTGTTTCCCCTGCATGT NAG 2 0.162 Tier II
17 NC_000075.6 9 107693133 - Sema3f NNNCTGTGTCCCCTGCATGG NGG 2 0.1142 Tier II
18 NC_000073.6 7 120008632 + Dnah3 NNNTTGTGTCCCCTGGATAT NGG 2 0.1099 Tier II
19 NC_000078.6 12 116366246 + Esyt2 NNNTTGTGTCACCTGCATGC NGG 2 0.0682 Tier II
20 NC_000068.7 2 134974291 - Plcb1 NNNTTGTGTCTCCTGCATGG NGG 2 0.0543 Tier II
21 NC_000085.6 19 22862461 + Trpm3 NNNTTGTGGCCCCTGCATGT NGA 2 0.043 Tier II
22 NC_000072.6 6 103536220 + Chl1 NNNTTGTATCCCCTGCATGT NTG 2 0.039 Tier II
23 NC_000080.6 14 24464125 - Polr3a NNNTTGTGTCCCCTTCATGT NAG 2 0.037 Tier II
24 NC_000077.6 11 19997078 - Spred2 NNNTTCTGTCCCCTGCATGT NTG 2 0.0266 Tier II
25 NC_000077.6 11 60147331 + Rai1 NNNTTGTGTCCCCTTCCTGT NGG 2 0.0252 Tier II
26 NC_000075.6 9 67311142 + Tln2 NNNGTGTGTCCCCTGCATGT NGC 2 0.0139 Tier II
27 NC_000073.6 7 132679185 - Lhpp NNNTTGGGTCCCCTGCATGT NGT 2 0.0095 Tier II
28 NC_000072.6 6 96117272 - 2900060L22Rik NNNTTGTGTTCCCTCCATGT NGG 2 0.2567 Tier III
29 NC_000073.6 7 83609281 - Gm16638 NNNTTGTGGGCCCTGCATGT NGG 2 0.2407 Tier III
30 NC_000085.6 19 43627562 + Gm34922 NNNTTGTGTCCCCTGTAAGT NGG 2 0.2051 Tier III
31 NC_000071.6 5 117002329 + Gm30028 NNNTTCTGTCCCCTGCATGT NAG 2 0.1768 Tier III
Download CSV

Other clones with same target sequence:

(none)