Construct: sgRNA BRDN0001147649
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGCCGCATCCTGTTATATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80187
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
168514326 |
+ |
RARS1 |
NNNCCGCATTCTGTTATTTT |
NGG |
2 |
0.4706 |
Tier II |
| 2 |
NC_000006.12 |
6 |
25792148 |
+ |
SLC17A1 |
NNNCTGCATCCTTTTATATT |
NGG |
2 |
0.1909 |
Tier II |
| 3 |
NC_000020.11 |
20 |
52410343 |
+ |
LOC105372666 |
NNNCCACATCCTGTTATGTT |
NGG |
2 |
0.4 |
Tier III |
| 4 |
NC_000017.11 |
17 |
72427574 |
+ |
LINC00673 |
NNNCCACATCCTGTTTTATT |
NGG |
2 |
0.0 |
Tier III |
| 5 |
NC_000020.11 |
20 |
55720603 |
+ |
LOC107984001 |
NNNCCGCATCCTGTTTTATC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
35016925 |
+ |
Kirrel3 |
NNNCCACTTCCTGTTATATT |
NGG |
2 |
0.4286 |
Tier II |
| 2 |
NC_000084.6 |
18 |
20534312 |
- |
Dsg3 |
NNNCAGCATCCTTTTATATT |
NGG |
2 |
0.1714 |
Tier II |
| 3 |
NC_000084.6 |
18 |
60944381 |
+ |
Camk2a |
NNNCAGCATCCTGTTTTATT |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000073.6 |
7 |
139390678 |
- |
Inpp5a |
NNNCCGCATCCTGCTTTATT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
