Construct: sgRNA BRDN0001147653

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGAAGAAGAATTGCATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K3 (8491)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76406

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 39293235 - MAP4K3 NNNAAGAAGAATTGCATCAG NGG 0 1.0 Tier I
2 NC_000005.10 5 178964723 + ZNF454 NNNAAGAAGAATTGGATCAA NGG 2 0.0469 Tier I
3 NC_000010.11 10 119058148 - EIF3A NNNAAGAAGAATTGGAACAG NGG 2 0.0267 Tier I
4 NC_000005.10 5 111507152 - STARD4 NNNAGGAAGAAATGCATCAG NGG 2 0.576 Tier II
5 NC_000013.11 13 111124219 - ARHGEF7 NNNAAAAAGAATTGCATAAG NGG 2 0.5385 Tier II
6 NC_000011.10 11 79209230 + TENM4 NNNAAGAAGGATTGCATCAA NGG 2 0.5208 Tier II
7 NC_000020.11 20 7935042 - HAO1 NNNAAGAAAAAATGCATCAG NGG 2 0.5143 Tier II
8 NC_000002.12 2 110443362 - LIMS4 NNNAAAAAGAAGTGCATCAG NGG 2 0.5 Tier II
9 NC_000006.12 6 42266180 - TRERF1 NNNAAAAAGAATTCCATCAG NGG 2 0.4286 Tier II
10 NC_000014.9 14 32984944 + NPAS3 NNNAAGGACAATTGCATCAG NGG 2 0.3801 Tier II
11 NC_000016.10 16 83226973 + CDH13 NNNAAGAAGAAGTGCATTAG NGG 2 0.3214 Tier II
12 NC_000001.11 1 236202091 - GPR137B NNNAAGATGAATTGCATCAC NGG 2 0.1837 Tier II
13 NC_000008.11 8 68698100 - C8orf34 NNNAAGAAGAAATGAATCAG NGG 2 0.1778 Tier II
14 NC_000007.14 7 150203249 + ACTR3C NNNAAGAAGCATTGCAACAG NGG 2 0.1778 Tier II
15 NC_000007.14 7 153765881 - DPP6 NNNAAGAAGCATTGCAACAG NGG 2 0.1778 Tier II
16 NC_000011.10 11 70073147 + ANO1 NNNAAGAAGAATTGAACCAG NGG 2 0.1569 Tier II
17 NC_000017.11 17 8572297 - MYH10 NNNAAGAAGAATAGAATCAG NGG 2 0.1538 Tier II
18 NC_000012.12 12 70833863 - PTPRR NNNAAGAAGAATTGCATGGG NGG 2 0.05 Tier II
19 NC_000004.12 4 55461748 - CLOCK NNNAAGAAGACTTGCAGCAG NGG 2 0.0471 Tier II
20 NC_000014.9 14 92743049 - LGMN NNNAAGAAAAATTGCATCAG NTG 2 0.025 Tier II
21 NC_000017.11 17 32776875 - MYO1D NNNAAGAAGAATTTTATCAG NGG 2 0.0178 Tier II
22 NC_000003.12 3 17348556 - TBC1D5 NNNAAGAAGAATTGTATGAG NGG 2 0.0089 Tier II
23 NC_000002.12 2 172844686 + RAPGEF4 NNNAAGAAGAATTTCCTCAG NGG 2 0.0 Tier II
24 NC_000008.11 8 124628777 - MTSS1 NNNATGAAGAATTGCCTCAG NGG 2 0.0 Tier II
25 NC_000002.12 2 109928147 + LIMS3-LOC440895 NNNAAAAAGAAGTGCATCAG NGG 2 0.5 Tier III
26 NC_000017.11 17 10737085 - TMEM220-AS1 NNNAAGAAGAATGGCATCAA NGG 2 0.2446 Tier III
27 NC_000011.10 11 70073147 + LINC02584 NNNAAGAAGAATTGAACCAG NGG 2 0.1569 Tier III
28 NC_000007.14 7 7275450 - LINC03016 NNNAAGAAGAATTGAATTAG NGG 2 0.1429 Tier III
29 NC_000007.14 7 7275450 - LOC107986764 NNNAAGAAGAATTGAATTAG NGG 2 0.1429 Tier III
30 NC_000003.12 3 194228617 - LINC02036 NNNAAGAAGAATTGCATGAG NTG 2 0.0052 Tier III
31 NC_000006.12 6 53939527 - LOC101927189 NNNAAGAAGAATTGGATCAG NGT 2 0.0008 Tier III
32 NC_000022.11 22 47468940 - LINC01644 NNNAAGAAGACTTGCTTCAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 80618562 - Map4k3 NNNAAGAGGAATTACATCAG NGG 2 0.55 Tier I
2 NC_000067.6 1 128300156 - Lct NNNGAGAAGAATTACATCAG NGG 2 0.4688 Tier I
3 NC_000072.6 6 132868150 + Tas2r110 NNNAAGAAGCATTTCATCAG NGG 2 0.0889 Tier I
4 NC_000072.6 6 120476911 + Il17ra NNNAAGAAGAATTCCATCAG NGA 2 0.0298 Tier I
5 NC_000085.6 19 31191424 + Prkg1 NNNAGGAAGAATCGCATCAG NGG 2 0.5684 Tier II
6 NC_000083.6 17 58094447 + Cntnap5c NNNAAGAAGAAAAGCATCAG NGG 2 0.5538 Tier II
7 NC_000072.6 6 38978529 - Tbxas1 NNNAAGAAGAACTGCATAAG NGG 2 0.5101 Tier II
8 NC_000071.6 5 81250454 - Adgrl3 NNNTAGAAGAATCGCATCAG NGG 2 0.5024 Tier II
9 NC_000073.6 7 79310856 - Abhd2 NNNAAAAAGAAGTGCATCAG NGG 2 0.5 Tier II
10 NC_000075.6 9 95642012 - Pcolce2 NNNAAGAACTATTGCATCAG NGG 2 0.4751 Tier II
11 NC_000070.6 4 34771823 + Smim8 NNNAAGAAGAAGAGCATCAG NGG 2 0.3462 Tier II
12 NC_000078.6 12 100590043 + Rps6ka5 NNNAACTAGAATTGCATCAG NGG 2 0.2983 Tier II
13 NC_000074.6 8 99147124 - Cdh8 NNNTAGAAGAATTGCATCAC NGG 2 0.2727 Tier II
14 NC_000079.6 13 17779905 - Cdk13 NNNCAGAAGAATAGCATCAG NGG 2 0.2443 Tier II
15 NC_000068.7 2 127767333 + Nphp1 NNNAAGAACAATTCCATCAG NGG 2 0.2308 Tier II
16 NC_000068.7 2 5044900 - Optn NNNAAGAAACATTGCATCAG NGG 2 0.2143 Tier II
17 NC_000076.6 10 87913553 + Igf1 NNNAAGAAGAAAGGCATCAG NGG 2 0.2087 Tier II
18 NC_000070.6 4 66023377 + Astn2 NNNGAGAAGCATTGCATCAG NGG 2 0.2083 Tier II
19 NC_000077.6 11 33459951 + Ranbp17 NNNAAGTAGAATTCCATCAG NGG 2 0.1875 Tier II
20 NC_000084.6 18 44764736 - Mcc NNNAAGAAGCATTGCATCTG NGG 2 0.1795 Tier II
21 NC_000086.7 X 82898499 + Dmd NNNAAGAAGAGTGGCATCAG NGG 2 0.1696 Tier II
22 NC_000067.6 1 136075187 + Cacna1s NNNAAGAAGAATGGCATCTG NGG 2 0.1405 Tier II
23 NC_000080.6 14 70743771 + Xpo7 NNNAAGAAGACTTGCATGAG NGG 2 0.0533 Tier II
24 NC_000071.6 5 89824314 + Adamts3 NNNAAGAAGAATAGTATCAG NGG 2 0.0462 Tier II
25 NC_000069.6 3 45398517 - Pcdh10 NNNAAGAATAATTGTATCAG NGG 2 0.0356 Tier II
26 NC_000075.6 9 28151851 - Opcml NNNAAAAAGAATTGCATCAG NGC 2 0.0222 Tier II
27 NC_000085.6 19 21004282 - Tmc1 NNNAAGAAGAATTACATCAG NGT 2 0.0121 Tier II
28 NC_000082.6 16 34784313 - Mylk NNNAAGAAGAATGGCCTCAG NGG 2 0.0 Tier II
29 NC_000085.6 19 52940638 + Xpnpep1 NNNAAGAAGAATTCCCTCAG NGG 2 0.0 Tier II
30 NC_000072.6 6 8036157 + Col28a1 NNNAAGAAGACTTGCTTCAG NGG 2 0.0 Tier II
31 NC_000069.6 3 137297281 + Gm567 NNNTAGAAGAAATGCATCAG NGG 2 0.5091 Tier III
32 NC_000079.6 13 10437058 + Gm26861 NNNTAGAAGAATTGCATAAG NGG 2 0.3427 Tier III
33 NC_000082.6 16 31350043 + Gm41443 NNNAAGAATAATTGCATCTG NGG 2 0.2872 Tier III
34 NC_000074.6 8 99147124 - Gm15681 NNNTAGAAGAATTGCATCAC NGG 2 0.2727 Tier III
35 NC_000072.6 6 68621043 - Igk NNNAAGAAGAATTGGATCAA NGG 2 0.0469 Tier III
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Other clones with same target sequence:

(none)