Construct: sgRNA BRDN0001147654
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTACTGGCATCTAGATCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKMT1B (1159)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76593
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
43595453 |
- |
CKMT1B |
NNNACTGGCATCTAGATCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43695285 |
- |
CKMT1A |
NNNACTGGCATCTAGATCCG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000016.10 |
16 |
82840747 |
- |
CDH13 |
NNNACTGTCATCTAGATCCT |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000012.12 |
12 |
80220723 |
+ |
OTOGL |
NNNACTGGCATCTAGATCCC |
NGG |
1 |
0.4286 |
Tier II |
| 5 |
NC_000013.11 |
13 |
51663375 |
- |
WDFY2 |
NNNCCTGGCATCTAGATCTG |
NGG |
2 |
0.1629 |
Tier II |
| 6 |
NC_000002.12 |
2 |
200434968 |
+ |
SPATS2L |
NNNACTGGCAGCTAGATCGG |
NGG |
2 |
0.05 |
Tier II |
| 7 |
NC_000006.12 |
6 |
150244918 |
- |
PPP1R14C |
NNNACTGGCATCTAGATCAG |
NGC |
2 |
0.0095 |
Tier II |
| 8 |
NC_000003.12 |
3 |
13220854 |
+ |
IQSEC1 |
NNNACTGGCATCTAGATCTG |
NGT |
2 |
0.0074 |
Tier II |
| 9 |
NC_000002.12 |
2 |
200434968 |
+ |
LOC101927741 |
NNNACTGGCAGCTAGATCGG |
NGG |
2 |
0.05 |
Tier III |
| 10 |
NC_000006.12 |
6 |
21902701 |
- |
CASC15 |
NNNACTAGCATCTAGTTCCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
31169584 |
+ |
Acap2 |
NNNACTGGCATCTAGATCCC |
NAG |
2 |
0.1111 |
Tier II |
| 2 |
NC_000069.6 |
3 |
103172619 |
- |
Bcas2 |
NNNCCTGGCATCTAGATCCG |
NAG |
2 |
0.0915 |
Tier II |
| 3 |
NC_000084.6 |
18 |
45663549 |
- |
Kcnn2 |
NNNACTGGCATCTAGAGCCT |
NGG |
2 |
0.0824 |
Tier II |
| 4 |
NC_000068.7 |
2 |
145725946 |
- |
BC039771 |
NNNACTGGCATTTAGATCCC |
NGG |
2 |
0.2308 |
Tier III |
| 5 |
NC_000086.7 |
X |
102132745 |
+ |
Gm16471 |
NNNACTGGCATCTAGAACCG |
NAG |
2 |
0.1383 |
Tier III |
| 6 |
NC_000084.6 |
18 |
45663549 |
- |
Gm46623 |
NNNACTGGCATCTAGAGCCT |
NGG |
2 |
0.0824 |
Tier III |
Other clones with same target sequence:
(none)
