Construct: sgRNA BRDN0001147656
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGTCTTGAGTCAATCACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MUSK (4593)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77503
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
110747682 |
- |
MUSK |
NNNGTCTTGAGTCAATCACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
49561135 |
- |
NOSIP |
NNNGCCTTGAGCCAATCACT |
NGG |
2 |
0.9474 |
Tier II |
| 3 |
NC_000010.11 |
10 |
122802946 |
- |
LOC124900290 |
NNNGCCTTGAGTCAAACACT |
NGG |
2 |
0.9091 |
Tier II |
| 4 |
NC_000009.12 |
9 |
114163850 |
+ |
COL27A1 |
NNNGTCCTGAGTCAGTCACT |
NGG |
2 |
0.4469 |
Tier II |
| 5 |
NC_000001.11 |
1 |
81374196 |
- |
ADGRL2 |
NNNGTTTTGAGTCAATAACT |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000003.12 |
3 |
11662015 |
- |
VGLL4 |
NNNTTCTTGAGCCAATCACT |
NGG |
2 |
0.3445 |
Tier II |
| 7 |
NC_000012.12 |
12 |
117280450 |
+ |
NOS1 |
NNNGTCTTGAGTAACTCACT |
NGG |
2 |
0.1049 |
Tier II |
| 8 |
NC_000008.11 |
8 |
60854075 |
+ |
CHD7 |
NNNCTCTTGAGTCAATGACT |
NGG |
2 |
0.0311 |
Tier II |
| 9 |
NC_000001.11 |
1 |
24200798 |
- |
LINC02800 |
NNNGTCTTTACTCAATCACT |
NGG |
2 |
0.2286 |
Tier III |
| 10 |
NC_000016.10 |
16 |
79816237 |
+ |
LINC01228 |
NNNGTCATGAGTCATTCACT |
NGG |
2 |
0.175 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
58344193 |
- |
Musk |
NNNGTCTTGAGTCAATCACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
31613110 |
- |
Fubp3 |
NNNGTCTTGAGTCACTTACT |
NGG |
2 |
0.1273 |
Tier I |
| 3 |
NC_000074.6 |
8 |
29065990 |
+ |
Unc5d |
NNNGTCATGAGCCAATCACT |
NGG |
2 |
0.8289 |
Tier II |
| 4 |
NC_000068.7 |
2 |
164986264 |
- |
Slc12a5 |
NNNGTCTTGAGCCAGTCACT |
NGG |
2 |
0.6158 |
Tier II |
| 5 |
NC_000081.6 |
15 |
71514031 |
- |
Fam135b |
NNNGTCTTAAGTCAATCACA |
NGG |
2 |
0.3616 |
Tier II |
| 6 |
NC_000077.6 |
11 |
77986822 |
- |
Phf12 |
NNNGTCCTGAGTCAATCCCT |
NGG |
2 |
0.131 |
Tier II |
| 7 |
NC_000068.7 |
2 |
164009156 |
- |
Ywhab |
NNNGTCTTGACTCACTCACT |
NGG |
2 |
0.1169 |
Tier II |
| 8 |
NC_000070.6 |
4 |
47240671 |
+ |
Col15a1 |
NNNGTCTAGAGTGAATCACT |
NGG |
2 |
0.1091 |
Tier II |
| 9 |
NC_000073.6 |
7 |
3648153 |
- |
Cnot3 |
NNNGTCTTGATTCAATCCCT |
NGG |
2 |
0.0733 |
Tier II |
| 10 |
NC_000074.6 |
8 |
125448307 |
+ |
Sipa1l2 |
NNNGTCTTTAGTCAATCACT |
NGT |
2 |
0.0086 |
Tier II |
| 11 |
NC_000070.6 |
4 |
47240671 |
+ |
Gm32317 |
NNNGTCTAGAGTGAATCACT |
NGG |
2 |
0.1091 |
Tier III |
| 12 |
NC_000083.6 |
17 |
9703912 |
+ |
Gm34684 |
NNNCTCTTGAGTGAATCACT |
NGG |
2 |
0.0722 |
Tier III |
Other clones with same target sequence:
(none)
