Construct: sgRNA BRDN0001147657
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCGAAGCCAATGTACCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AXL (558)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76701
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
41220748 |
- |
AXL |
NNNGAAGCCAATGTACCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
43387233 |
+ |
HNRNPF |
NNNGATGCCAATGTACCTCC |
NGG |
2 |
0.3061 |
Tier I |
| 3 |
NC_000001.11 |
1 |
24342865 |
- |
GRHL3 |
NNNGAAGCCAATGTGCCCCG |
NGG |
2 |
0.2786 |
Tier II |
| 4 |
NC_000013.11 |
13 |
100525758 |
+ |
PCCA |
NNNGAAGCCAATGTACTTGG |
NGG |
2 |
0.0583 |
Tier II |
| 5 |
NC_000002.12 |
2 |
97817477 |
+ |
TMEM131 |
NNNGAAGACAATGTACCTCG |
NGA |
2 |
0.0451 |
Tier II |
| 6 |
NC_000002.12 |
2 |
132108812 |
- |
VN1R19P |
NNNGAAGCCAATGTACCTTA |
NGG |
2 |
0.4327 |
Tier III |
| 7 |
NC_000013.11 |
13 |
18463624 |
+ |
VN1R58P |
NNNGAAGCCAATGTACCTTA |
NGG |
2 |
0.4327 |
Tier III |
| 8 |
NC_000018.10 |
18 |
15276695 |
- |
VN1R75P |
NNNGAAGCCAATGTACCTTA |
NGG |
2 |
0.4327 |
Tier III |
| 9 |
NC_000021.9 |
21 |
13091810 |
+ |
ANKRD30BP2 |
NNNGAAGCCAATGTACCTTA |
NGG |
2 |
0.4327 |
Tier III |
| 10 |
NC_000021.9 |
21 |
13091810 |
+ |
VN1R109P |
NNNGAAGCCAATGTACCTTA |
NGG |
2 |
0.4327 |
Tier III |
| 11 |
NC_000003.12 |
3 |
12258073 |
- |
GSTM5P1 |
NNNGAAGCCAAAGTACCTGG |
NGG |
2 |
0.1 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
4917932 |
+ |
Dpp3 |
NNNGAAGCCAATGTAACTAG |
NGG |
2 |
0.4286 |
Tier I |
| 2 |
NC_000075.6 |
9 |
54367959 |
- |
Dmxl2 |
NNNGAAGCCAAAGTCCCTCG |
NGG |
2 |
0.2182 |
Tier II |
| 3 |
NC_000072.6 |
6 |
99371781 |
+ |
Foxp1 |
NNNGAAGCCACTGTACCTCC |
NGG |
2 |
0.1714 |
Tier II |
Other clones with same target sequence:
(none)
