Construct: sgRNA BRDN0001147661
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGATCTAGCGTCCAACCAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKMT1B (1159)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76594
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
43595069 |
- |
CKMT1B |
NNNTCTAGCGTCCAACCAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43694901 |
- |
CKMT1A |
NNNTCTAGCGTCCAACCAGT |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000017.11 |
17 |
47699652 |
+ |
TBKBP1 |
NNNTCTGGCCTCCAACCAGT |
NGG |
2 |
0.2824 |
Tier I |
| 4 |
NC_000003.12 |
3 |
57887900 |
- |
SLMAP |
NNNTCTAGCTTCCAACCAGA |
NGG |
2 |
0.457 |
Tier II |
| 5 |
NC_000019.10 |
19 |
41220424 |
- |
AXL |
NNNTCTAGCGTCCATCCCGT |
NGG |
2 |
0.0381 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
121359676 |
- |
Ckmt1 |
NNNTCTAGTGTCCAACCAGT |
NGG |
1 |
0.875 |
Tier I |
| 2 |
NC_000077.6 |
11 |
97146346 |
- |
Tbkbp1 |
NNNTCTGGCCTCCAACCAGT |
NGG |
2 |
0.2824 |
Tier I |
| 3 |
NC_000080.6 |
14 |
121505093 |
+ |
Slc15a1 |
NNNTCCAGCCTCCAACCAGT |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000067.6 |
1 |
151753879 |
- |
2810414N06Rik |
NNNCCTAGTGTCCAACCAGT |
NGG |
2 |
0.5662 |
Tier III |
Other clones with same target sequence:
(none)
