Construct: sgRNA BRDN0001147662
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTAGTGCATTCAAGCACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KIT (3815)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77562
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
54725965 |
+ |
KIT |
NNNAGTGCATTCAAGCACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
31020268 |
+ |
ZNF438 |
NNNAGTTTATTCAAGCACAA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000003.12 |
3 |
146536721 |
- |
PLSCR1 |
NNNAATGCAGTCAAGCACAA |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000013.11 |
13 |
43522703 |
- |
ENOX1 |
NNNAGTGCACTAAAGCACAA |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000001.11 |
1 |
89170577 |
+ |
GBP7 |
NNNAGTGCATCCAAGCACTA |
NGG |
2 |
0.359 |
Tier II |
| 6 |
NC_000015.10 |
15 |
76647478 |
- |
SCAPER |
NNNAGTGCCTTCAGGCACAA |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000004.12 |
4 |
154279545 |
+ |
DCHS2 |
NNNAGTCCATTCAACCACAA |
NGG |
2 |
0.1875 |
Tier II |
| 8 |
NC_000006.12 |
6 |
149390683 |
- |
TAB2 |
NNNAGTGAATTCAAGCACCA |
NGG |
2 |
0.1345 |
Tier II |
| 9 |
NC_000006.12 |
6 |
125867533 |
+ |
NCOA7 |
NNNAGTGCATTCAAGCACAG |
NGA |
2 |
0.0531 |
Tier II |
| 10 |
NC_000009.12 |
9 |
101389713 |
+ |
MRPL50 |
NNNAGTGCATTAAAGCACAA |
NGA |
2 |
0.0496 |
Tier II |
| 11 |
NC_000009.12 |
9 |
87637758 |
+ |
DAPK1 |
NNNAGTGCTTTCAAGCACAA |
NTG |
2 |
0.0234 |
Tier II |
| 12 |
NC_000006.12 |
6 |
6558174 |
- |
LY86-AS1 |
NNNAGTGCATGCAAGCACAA |
NGG |
1 |
0.4 |
Tier III |
| 13 |
NC_000006.12 |
6 |
91655584 |
- |
CASC6 |
NNNAGTGCAATGAAGCACAA |
NGG |
2 |
0.381 |
Tier III |
| 14 |
NC_000001.11 |
1 |
89170577 |
+ |
LOC105378842 |
NNNAGTGCATCCAAGCACTA |
NGG |
2 |
0.359 |
Tier III |
| 15 |
NC_000023.11 |
X |
103531835 |
+ |
LINC02589 |
NNNACTGCATTGAAGCACAA |
NGG |
2 |
0.3492 |
Tier III |
| 16 |
NC_000003.12 |
3 |
18180161 |
+ |
BALR6 |
NNNAGGGCATTCAAGCAAAA |
NGG |
2 |
0.3077 |
Tier III |
| 17 |
NC_000003.12 |
3 |
18180161 |
+ |
LOC124909351 |
NNNAGGGCATTCAAGCAAAA |
NGG |
2 |
0.3077 |
Tier III |
| 18 |
NC_000004.12 |
4 |
154279545 |
+ |
LOC101927947 |
NNNAGTCCATTCAACCACAA |
NGG |
2 |
0.1875 |
Tier III |
| 19 |
NC_000018.10 |
18 |
49515806 |
- |
LINC02837 |
NNNAGTGCATTCAGGCAGAA |
NGG |
2 |
0.0622 |
Tier III |
| 20 |
NC_000017.11 |
17 |
8709359 |
- |
LOC105371525 |
NNNAGTCCATTCAAGCACAA |
NGA |
2 |
0.0477 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
44572096 |
+ |
Gulp1 |
NNNAATGCATTCAAACACAA |
NGG |
2 |
0.8157 |
Tier II |
| 2 |
NC_000086.7 |
X |
93307845 |
- |
Pola1 |
NNNAGTGCATTCAAGCACTA |
NGG |
1 |
0.5385 |
Tier II |
| 3 |
NC_000077.6 |
11 |
103463716 |
- |
Lrrc37a |
NNNAATGCATTCAAGCACAT |
NGG |
2 |
0.52 |
Tier II |
| 4 |
NC_000084.6 |
18 |
69404878 |
- |
Tcf4 |
NNNTCTGCATTCAAGCACAA |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000076.6 |
10 |
123107717 |
- |
Usp15 |
NNNAGGGCAGTCAAGCACAA |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000080.6 |
14 |
117031399 |
- |
Gpc6 |
NNNAGTGCATTCAACAACAA |
NGG |
2 |
0.2727 |
Tier II |
| 7 |
NC_000076.6 |
10 |
105800843 |
+ |
Mettl25 |
NNNAGTGCATTCAAGAACAA |
NAG |
2 |
0.2593 |
Tier II |
| 8 |
NC_000071.6 |
5 |
32259541 |
- |
Plb1 |
NNNATTCCATTCAAGCACAA |
NGG |
2 |
0.2063 |
Tier II |
| 9 |
NC_000073.6 |
7 |
44752991 |
- |
Vrk3 |
NNNAGTGCATTCAATCACAT |
NGG |
2 |
0.0857 |
Tier II |
| 10 |
NC_000074.6 |
8 |
62098125 |
+ |
Anxa10 |
NNNAGTGCATTCATGCACAA |
NTG |
2 |
0.0208 |
Tier II |
| 11 |
NC_000072.6 |
6 |
97885580 |
- |
Mitf |
NNNAGTGCATGCAAGCACAA |
NTG |
2 |
0.0156 |
Tier II |
| 12 |
NC_000079.6 |
13 |
7456423 |
- |
Gm36074 |
NNNAGTGCATCCAGGCACAA |
NGG |
2 |
0.3111 |
Tier III |
| 13 |
NC_000080.6 |
14 |
100470594 |
- |
Gm34199 |
NNNAGTGCATTCATGCACAA |
NGT |
2 |
0.0086 |
Tier III |
Other clones with same target sequence:
(none)
