Construct: sgRNA BRDN0001147663
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCCTCATAGAATTCCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK2 (156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75683
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
67279257 |
- |
GRK2 |
NNNCTCATAGAATTCCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
153320488 |
+ |
PNMA6F |
NNNCTCAGCGAATTCCACCA |
NGG |
2 |
0.419 |
Tier I |
| 3 |
NC_000015.10 |
15 |
48444789 |
+ |
FBN1 |
NNNTTCATAAAATTCCACCA |
NGG |
2 |
0.7467 |
Tier II |
| 4 |
NC_000010.11 |
10 |
74135313 |
+ |
AP3M1 |
NNNCTCATACAGTTCCACCA |
NGG |
2 |
0.2889 |
Tier II |
| 5 |
NC_000023.11 |
X |
123727366 |
+ |
THOC2 |
NNNCTAATAGAAGTCCACCA |
NGG |
2 |
0.2422 |
Tier II |
| 6 |
NC_000007.14 |
7 |
151503525 |
- |
RHEB |
NNNCTGTTAGAATTCCACCA |
NGG |
2 |
0.2188 |
Tier II |
| 7 |
NC_000011.10 |
11 |
128517602 |
+ |
ETS1 |
NNNCTCATGGAATTCCACCA |
NAG |
2 |
0.1728 |
Tier II |
| 8 |
NC_000002.12 |
2 |
201855511 |
- |
CDK15 |
NNNCTCAAAGAATTCCCCCA |
NGG |
2 |
0.1412 |
Tier II |
| 9 |
NC_000001.11 |
1 |
185787754 |
- |
HMCN1 |
NNNCTCATAGAATTACACAA |
NGG |
2 |
0.0952 |
Tier II |
| 10 |
NC_000020.11 |
20 |
24649347 |
- |
SYNDIG1 |
NNNCTCATAGATTTCCAGCA |
NGG |
2 |
0.0444 |
Tier II |
| 11 |
NC_000002.12 |
2 |
29602326 |
+ |
ALK |
NNNCTCATAGAATCCCAGCA |
NGG |
2 |
0.0381 |
Tier II |
| 12 |
NC_000010.11 |
10 |
11239150 |
+ |
CELF2 |
NNNCTCATAGAATTCCACCC |
NGA |
2 |
0.0158 |
Tier II |
| 13 |
NC_000002.12 |
2 |
165927743 |
- |
TTC21B |
NNNCTCATAGAATTGTACCA |
NGG |
2 |
0.0154 |
Tier II |
| 14 |
NC_000023.11 |
X |
19458860 |
- |
MAP3K15 |
NNNCTCATAGAATTCCACAA |
NGC |
2 |
0.0095 |
Tier II |
| 15 |
NC_000023.11 |
X |
153028984 |
- |
LOC112268308 |
NNNCTCAGCGAATTCCACCA |
NGG |
2 |
0.419 |
Tier III |
| 16 |
NC_000003.12 |
3 |
99468506 |
- |
LOC105374007 |
NNNCTGAAAGAATTCCACCA |
NGG |
2 |
0.4 |
Tier III |
| 17 |
NC_000007.14 |
7 |
151503525 |
- |
ETF1P2 |
NNNCTGTTAGAATTCCACCA |
NGG |
2 |
0.2188 |
Tier III |
| 18 |
NC_000002.12 |
2 |
11402556 |
+ |
LINC00570 |
NNNCTCATAGAATTCCCCCA |
NGG |
1 |
0.1765 |
Tier III |
| 19 |
NC_000007.14 |
7 |
142323230 |
- |
TRB |
NNNCTCATAAAATGCCACCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
129387540 |
- |
Plpp4 |
NNNCTAATGGAATTCCACCA |
NGG |
2 |
0.619 |
Tier II |
| 2 |
NC_000071.6 |
5 |
127904973 |
+ |
Tmem132d |
NNNATCATAGAATTCCATCA |
NGG |
2 |
0.5414 |
Tier II |
| 3 |
NC_000070.6 |
4 |
128379748 |
- |
Csmd2 |
NNNCTCACAGAATTCCAACA |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000084.6 |
18 |
43035194 |
+ |
Ppp2r2b |
NNNTACATAGAATTCCACCA |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000074.6 |
8 |
14768009 |
+ |
Dlgap2 |
NNNCTCAAAGAATTCCACAA |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000067.6 |
1 |
146779988 |
- |
Brinp3 |
NNNATCATAGCATTCCACCA |
NGG |
2 |
0.3368 |
Tier II |
| 7 |
NC_000075.6 |
9 |
67240420 |
- |
Tln2 |
NNNCACATGGAATTCCACCA |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000078.6 |
12 |
105007193 |
- |
Syne3 |
NNNCACATCGAATTCCACCA |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000067.6 |
1 |
91370879 |
- |
Erfe |
NNNCTCATGGAATTCCACAA |
NGG |
2 |
0.2857 |
Tier II |
| 10 |
NC_000077.6 |
11 |
98357517 |
+ |
Ppp1r1b |
NNNCTCATAGACTTCAACCA |
NGG |
2 |
0.2632 |
Tier II |
| 11 |
NC_000077.6 |
11 |
8463742 |
- |
Tns3 |
NNNCTCATAGTATTCCACCT |
NGG |
2 |
0.1846 |
Tier II |
| 12 |
NC_000068.7 |
2 |
52431723 |
+ |
Cacnb4 |
NNNCTCATAGAATTCTAACA |
NGG |
2 |
0.1657 |
Tier II |
| 13 |
NC_000082.6 |
16 |
23295662 |
+ |
St6gal1 |
NNNCTCAGAGAATTACACCA |
NGG |
2 |
0.163 |
Tier II |
| 14 |
NC_000076.6 |
10 |
49295082 |
+ |
Grik2 |
NNNTTCATAGAATTCCTCCA |
NGG |
2 |
0.1067 |
Tier II |
| 15 |
NC_000080.6 |
14 |
17694408 |
+ |
Thrb |
NNNCTCAGAGAATTCCAGCA |
NGG |
2 |
0.0978 |
Tier II |
| 16 |
NC_000083.6 |
17 |
53574739 |
- |
Kat2b |
NNNCTCATAGAATTACCCCA |
NGG |
2 |
0.0392 |
Tier II |
| 17 |
NC_000071.6 |
5 |
44529054 |
- |
Ldb2 |
NNNCTCCTAGAATTTCACCA |
NGG |
2 |
0.0292 |
Tier II |
| 18 |
NC_000077.6 |
11 |
77408027 |
- |
Ssh2 |
NNNCTCATAGCATGCCACCA |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000077.6 |
11 |
98357517 |
+ |
1700003D09Rik |
NNNCTCATAGACTTCAACCA |
NGG |
2 |
0.2632 |
Tier III |
| 20 |
NC_000068.7 |
2 |
52431723 |
+ |
A430018G15Rik |
NNNCTCATAGAATTCTAACA |
NGG |
2 |
0.1657 |
Tier III |
| 21 |
NC_000069.6 |
3 |
52532906 |
- |
Gm30173 |
NNNCTCATAGAATCCCAACA |
NGG |
2 |
0.1538 |
Tier III |
| 22 |
NC_000069.6 |
3 |
34869563 |
+ |
Gm21388 |
NNNCTCATAGAAATTCACCA |
NGG |
2 |
0.0462 |
Tier III |
Other clones with same target sequence:
(none)
