Construct: sgRNA BRDN0001147665
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGCAGGTGCTGTTATTCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GK2 (2712)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77909
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
79407214 |
- |
GK2 |
NNNCAGGTGCTGTTATTCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
165278659 |
- |
GK3 |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier I |
| 3 |
NC_000023.11 |
X |
30718568 |
+ |
GK |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier I |
| 4 |
NC_000011.10 |
11 |
73010704 |
+ |
FCHSD2 |
NNNCAGGTGCAGTTATTAGT |
NGG |
2 |
0.4038 |
Tier II |
| 5 |
NC_000015.10 |
15 |
42488554 |
+ |
ZNF106 |
NNNCAGGTGCTCTTATTCAT |
NGG |
2 |
0.3782 |
Tier II |
| 6 |
NC_000003.12 |
3 |
178725723 |
+ |
KCNMB2 |
NNNCATGTGCTGTTATTCGA |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000001.11 |
1 |
75929929 |
+ |
ASB17 |
NNNCAGGTGCTGTTAATCGG |
NGG |
2 |
0.1604 |
Tier II |
| 8 |
NC_000004.12 |
4 |
165278659 |
- |
KLHL2 |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier II |
| 9 |
NC_000023.11 |
X |
54633121 |
- |
GNL3L |
NNNCAGGTGCTGGTATTCGT |
NGC |
2 |
0.0058 |
Tier II |
| 10 |
NC_000003.12 |
3 |
178725723 |
+ |
KCNMB2-AS1 |
NNNCATGTGCTGTTATTCGA |
NGG |
2 |
0.375 |
Tier III |
| 11 |
NT_113888.1 |
14 |
87898 |
+ |
MAFIP |
NNNCAGGTACTCTTATTCGT |
NGG |
2 |
0.3403 |
Tier III |
| 12 |
NC_000004.12 |
4 |
119393971 |
- |
GK6P |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier III |
| 13 |
NC_000004.12 |
4 |
119393971 |
- |
LOC107986193 |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier III |
| 14 |
NC_000023.11 |
X |
101779554 |
- |
GK4P |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
21653479 |
- |
Armc10 |
NNNCAGGTGCTGATAGTCGT |
NGG |
2 |
0.2396 |
Tier I |
| 2 |
NC_000079.6 |
13 |
49521035 |
- |
Ecm2 |
NNNCTGGTGCTGTTATTGGT |
NGG |
2 |
0.0485 |
Tier I |
| 3 |
NC_000071.6 |
5 |
97455991 |
- |
Gk2 |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier I |
| 4 |
NC_000069.6 |
3 |
67514992 |
+ |
Rarres1 |
NNNCAGGGGCTGTTATTCAT |
NGG |
2 |
0.5238 |
Tier II |
| 5 |
NC_000068.7 |
2 |
42269359 |
- |
Lrp1b |
NNNCAGGTCCAGTTATTCGT |
NGG |
2 |
0.4038 |
Tier II |
| 6 |
NC_000075.6 |
9 |
117422971 |
- |
Rbms3 |
NNNCAGGTCCAGTTATTCGT |
NGG |
2 |
0.4038 |
Tier II |
| 7 |
NC_000079.6 |
13 |
89041212 |
+ |
Edil3 |
NNNCAGGTCCAGTTATTCGT |
NGG |
2 |
0.4038 |
Tier II |
| 8 |
NC_000080.6 |
14 |
51483074 |
+ |
Gm7247 |
NNNCAGGTCCAGTTATTCGT |
NGG |
2 |
0.4038 |
Tier II |
| 9 |
NC_000085.6 |
19 |
39361768 |
+ |
Cyp2c39 |
NNNCAGGTCCAGTTATTCGT |
NGG |
2 |
0.4038 |
Tier II |
| 10 |
NC_000068.7 |
2 |
37601218 |
- |
Strbp |
NNNCAGGTGCTATTTTTCGT |
NGG |
2 |
0.1867 |
Tier II |
| 11 |
NC_000080.6 |
14 |
75930940 |
+ |
Gtf2f2 |
NNNCAGGTGCTGGTAGTCGT |
NGG |
2 |
0.0903 |
Tier II |
| 12 |
NC_000079.6 |
13 |
49521035 |
- |
Cenpp |
NNNCTGGTGCTGTTATTGGT |
NGG |
2 |
0.0485 |
Tier II |
| 13 |
NC_000069.6 |
3 |
32347704 |
+ |
Zmat3 |
NNNCAGCTGCTGTTATTCGT |
NTG |
2 |
0.0268 |
Tier II |
| 14 |
NC_000075.6 |
9 |
73204850 |
+ |
Gm27211 |
NNNCAGGTGCTATTATTTGT |
NGG |
2 |
0.6 |
Tier III |
| 15 |
NC_000081.6 |
15 |
24303593 |
- |
Gm36147 |
NNNCAGGTTCAGTTATTCGT |
NGG |
2 |
0.4 |
Tier III |
| 16 |
NC_000067.6 |
1 |
33173538 |
- |
Gm39616 |
NNNCAGATGCTGTTATTGGT |
NGG |
2 |
0.1333 |
Tier III |
| 17 |
NC_000069.6 |
3 |
58666093 |
+ |
LOC100417266 |
NNNCAGGTGCTGGTAGTCGT |
NGG |
2 |
0.0903 |
Tier III |
| 18 |
NC_000080.6 |
14 |
75930940 |
+ |
Gm9209 |
NNNCAGGTGCTGGTAGTCGT |
NGG |
2 |
0.0903 |
Tier III |
| 19 |
NC_000071.6 |
5 |
97455991 |
- |
4930467D21Rik |
NNNCTGGTGCTGTTATTCGC |
NGG |
2 |
0.0331 |
Tier III |
| 20 |
NC_000075.6 |
9 |
69786343 |
- |
B230323A14Rik |
NNNCAGGTGCTGTTATTGGG |
NGG |
2 |
0.0235 |
Tier III |
Other clones with same target sequence:
(none)
