Construct: sgRNA BRDN0001147666
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGCAAGCCATATACTGTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PEAK1 (79834)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75855
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
77180498 |
- |
PEAK1 |
NNNCAAGCCATATACTGTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
110772403 |
+ |
RBM20 |
NNNGAAGCCATATACAGTCG |
NGG |
2 |
0.4545 |
Tier II |
| 3 |
NC_000004.12 |
4 |
2674041 |
+ |
FAM193A |
NNNCAAGCCATATACGGTAG |
NGG |
2 |
0.1484 |
Tier II |
| 4 |
NC_000015.10 |
15 |
64491933 |
- |
ZNF609 |
NNNAAAGCCATATACTGTCG |
NGA |
2 |
0.0585 |
Tier II |
| 5 |
NC_000005.10 |
5 |
174341180 |
+ |
LINC01411 |
NNNCAAGTCATATACTGTCA |
NGG |
2 |
0.8203 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
93987180 |
- |
Cldn14 |
NNNCAAGCCATATACTGTCA |
NGG |
1 |
0.9375 |
Tier II |
| 2 |
NC_000073.6 |
7 |
131189338 |
+ |
Cdcp3 |
NNNCAAGCCAGATGCTGTCG |
NGG |
2 |
0.1867 |
Tier II |
| 3 |
NC_000070.6 |
4 |
50810387 |
+ |
Gm42287 |
NNNCAAGACATATACTGTGG |
NGG |
2 |
0.0813 |
Tier III |
| 4 |
NC_000070.6 |
4 |
50810387 |
+ |
Gm42288 |
NNNCAAGACATATACTGTGG |
NGG |
2 |
0.0813 |
Tier III |
Other clones with same target sequence:
(none)
