Construct: sgRNA BRDN0001147667
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTAGGTCTGATGTTCGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK3 (5595)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75968
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
30121950 |
+ |
MAPK3 |
NNNAGGTCTGATGTTCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
78446656 |
+ |
MAGI2 |
NNNAGTTCTGATGTTCAAAG |
NGG |
2 |
0.6222 |
Tier II |
| 3 |
NC_000001.11 |
1 |
167740715 |
- |
MPZL1 |
NNNAGGTCTGATGTTCAGAG |
NGG |
2 |
0.3733 |
Tier II |
| 4 |
NC_000003.12 |
3 |
69114209 |
+ |
LMOD3 |
NNNAGATCTGATGTTCCAAG |
NGG |
2 |
0.2353 |
Tier II |
| 5 |
NC_000007.14 |
7 |
99638509 |
- |
ZSCAN25 |
NNNAGGTCTGATCTTCGTAG |
NGG |
2 |
0.2105 |
Tier II |
| 6 |
NC_000004.12 |
4 |
184760123 |
- |
ACSL1 |
NNNAGGTCTGATGTACCAAG |
NGG |
2 |
0.1362 |
Tier II |
| 7 |
NC_000013.11 |
13 |
85382194 |
- |
LINC00351 |
NNNAGGTCAGATGTTTGAAG |
NGG |
2 |
0.2857 |
Tier III |
| 8 |
NC_000001.11 |
1 |
67913603 |
- |
GNG12-AS1 |
NNNAGGTCTGAAGTTTGAAG |
NGG |
2 |
0.2462 |
Tier III |
| 9 |
NC_000007.14 |
7 |
99638509 |
- |
LOC442603 |
NNNAGGTCTGATCTTCGTAG |
NGG |
2 |
0.2105 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
108483121 |
+ |
Eml1 |
NNNAGGTCTGATATTCAAAG |
NGG |
2 |
0.8615 |
Tier II |
| 2 |
NC_000083.6 |
17 |
57280952 |
+ |
Vav1 |
NNNAGGTCTTATGTACGAAG |
NGG |
2 |
0.4704 |
Tier II |
| 3 |
NC_000075.6 |
9 |
44826677 |
- |
Kmt2a |
NNNAGGTCTGATCTTCGAAG |
NAG |
2 |
0.1092 |
Tier II |
| 4 |
NC_000081.6 |
15 |
93741643 |
+ |
Gm30339 |
NNNGGGTCTGATGTTCGAAT |
NGG |
2 |
0.4375 |
Tier III |
Other clones with same target sequence:
(none)
