Construct: sgRNA BRDN0001147671
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGAGTTGAACTGAACCCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGR (2268)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75720
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
27616902 |
+ |
FGR |
NNNGTTGAACTGAACCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
10985279 |
+ |
CTNND2 |
NNNGGTGAACTGAACACGTG |
NGG |
2 |
0.64 |
Tier II |
| 3 |
NC_000006.12 |
6 |
89811534 |
- |
MDN1 |
NNNGTTGAACTGAACCCGAG |
NGG |
1 |
0.2857 |
Tier II |
| 4 |
NC_000006.12 |
6 |
162267322 |
- |
PRKN |
NNNTTTGAACTGAACCAGTG |
NGG |
2 |
0.1697 |
Tier II |
| 5 |
NC_000006.12 |
6 |
39647800 |
- |
KIF6 |
NNNCTTGAACTGAACCCGGG |
NGG |
2 |
0.1324 |
Tier II |
| 6 |
NC_000014.9 |
14 |
72824661 |
- |
DPF3 |
NNNCTTGAACTGAACCCGGG |
NGG |
2 |
0.1324 |
Tier II |
| 7 |
NC_000009.12 |
9 |
116846936 |
- |
ASTN2 |
NNNGTTGAACTGAACCCTTG |
NTG |
2 |
0.026 |
Tier II |
| 8 |
NC_000019.10 |
19 |
2763541 |
+ |
SGTA |
NNNGTTGAACTGAACCGGGG |
NGG |
2 |
0.0147 |
Tier II |
| 9 |
NC_000005.10 |
5 |
10985279 |
+ |
LOC105374654 |
NNNGGTGAACTGAACACGTG |
NGG |
2 |
0.64 |
Tier III |
| 10 |
NC_000017.11 |
17 |
13305141 |
- |
LINC02093 |
NNNGTTGAACTGAACCTGTG |
NGG |
1 |
0.4667 |
Tier III |
| 11 |
NC_000009.12 |
9 |
116846936 |
- |
LOC105376238 |
NNNGTTGAACTGAACCCTTG |
NTG |
2 |
0.026 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
121609862 |
- |
Lyzl4 |
NNNGTTGAACTGAACCAGTT |
NGG |
2 |
0.3267 |
Tier II |
| 2 |
NC_000073.6 |
7 |
45528379 |
+ |
Plekha4 |
NNNGCTGAACTGAACCCGTG |
NAG |
2 |
0.2593 |
Tier II |
| 3 |
NC_000086.7 |
X |
9992316 |
- |
Sytl5 |
NNNGTTGAACTGAACCCTAG |
NGG |
2 |
0.1905 |
Tier II |
| 4 |
NC_000075.6 |
9 |
121609862 |
- |
Lyzl4os |
NNNGTTGAACTGAACCAGTT |
NGG |
2 |
0.3267 |
Tier III |
| 5 |
NC_000072.6 |
6 |
29985721 |
- |
Smkr-ps |
NNNGTTGAACTGTACCGGTG |
NGG |
2 |
0.0176 |
Tier III |
Other clones with same target sequence:
(none)
