Construct: sgRNA BRDN0001147672
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTAACGGTGGTAATGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK13 (8621)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76625
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
39988204 |
- |
CDK13 |
NNNTAACGGTGGTAATGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
92415437 |
+ |
OMD |
NNNAAATGGTGGTAATGTAG |
NGG |
2 |
0.3869 |
Tier I |
| 3 |
NC_000021.9 |
21 |
36142609 |
+ |
CBR3 |
NNNTAACTGTAGTAATGTAG |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000009.12 |
9 |
92415437 |
+ |
CENPP |
NNNAAATGGTGGTAATGTAG |
NGG |
2 |
0.3869 |
Tier II |
| 5 |
NC_000023.11 |
X |
74880488 |
- |
NEXMIF |
NNNTAACGTTTGTAATGTAG |
NGG |
2 |
0.2051 |
Tier II |
| 6 |
NC_000012.12 |
12 |
93468225 |
+ |
MRPL42 |
NNNTAACGGTGTTAATGTAC |
NGG |
2 |
0.1648 |
Tier II |
| 7 |
NC_000021.9 |
21 |
36142609 |
+ |
CBR3-AS1 |
NNNTAACTGTAGTAATGTAG |
NGG |
2 |
0.625 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
175194860 |
- |
Rgs7 |
NNNTAACTGTGGAAATGTAG |
NGG |
2 |
0.4327 |
Tier II |
| 2 |
NC_000072.6 |
6 |
120094772 |
- |
Ninj2 |
NNNTAACTGTGGTACTGTAG |
NGG |
2 |
0.1705 |
Tier II |
| 3 |
NC_000071.6 |
5 |
144839721 |
+ |
Trrap |
NNNTAACGGTGGTAATGACG |
NGG |
2 |
0.1379 |
Tier II |
| 4 |
NC_000071.6 |
5 |
26162391 |
- |
Gm34067 |
NNNTAAGAGTGGTAATGTAG |
NGG |
2 |
0.4706 |
Tier III |
Other clones with same target sequence:
(none)
