Construct: sgRNA BRDN0001147680

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGACATGATCTGAGCTGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLK (51701)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75483

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 28161224 - NLK NNNACATGATCTGAGCTGAG NGG 0 1.0 Tier I
2 NC_000003.12 3 161232752 - NMD3 NNNAGATGATCTGAACTGAG NGG 2 0.5647 Tier II
3 NC_000005.10 5 170522508 + KCNIP1 NNNACATGATCTGAGCTAAT NGG 2 0.4846 Tier II
4 NC_000022.11 22 30975471 - TUG1 NNNACATGAACTGTGCTGAG NGG 2 0.4571 Tier II
5 NC_000020.11 20 63282967 + ARFGAP1 NNNACATGCTCAGAGCTGAG NGG 2 0.4571 Tier II
6 NC_000006.12 6 98899966 - FBXL4 NNNACATTTTCTGAGCTGAG NGG 2 0.375 Tier II
7 NC_000007.14 7 40395656 - SUGCT NNNACATTTTCTGAGCTGAG NGG 2 0.375 Tier II
8 NC_000020.11 20 4173817 + SMOX NNNACAGCATCTGAGCTGAG NGG 2 0.362 Tier II
9 NC_000011.10 11 61729156 - DAGLA NNNGCATGATCTGAGCAGAG NGG 2 0.3333 Tier II
10 NC_000006.12 6 108870371 + ARMC2 NNNGCATGATCGGAGCTGAG NGG 2 0.3125 Tier II
11 NC_000007.14 7 88866704 - ZNF804B NNNAGATGAGCTGAGCTGAG NGG 2 0.3 Tier II
12 NC_000004.12 4 6432833 + PPP2R2C NNNACATGAGCTGAGCTGTG NGG 2 0.2692 Tier II
13 NC_000022.11 22 32821604 + TIMP3 NNNAGATGATCTCAGCTGAG NGG 2 0.2526 Tier II
14 NC_000022.11 22 32821604 + SYN3 NNNAGATGATCTCAGCTGAG NGG 2 0.2526 Tier II
15 NC_000015.10 15 32785785 - FMN1 NNNACATGATCTGAGTCGAG NGG 2 0.2172 Tier II
16 NC_000017.11 17 16443329 + LRRC75A NNNCCATGAGCTGAGCTGAG NGG 2 0.1765 Tier II
17 NC_000006.12 6 4779822 - CDYL NNNACATGAGGTGAGCTGAG NGG 2 0.125 Tier II
18 NC_000001.11 1 167769001 + MPZL1 NNNACATGATCTCACCTGAG NGG 2 0.1148 Tier II
19 NC_000002.12 2 74093487 - TET3 NNNACATGACCTGAGGTGAG NGG 2 0.0821 Tier II
20 NC_000003.12 3 54334877 - CACNA2D3 NNNACAGGATCTGAGCTGAG NGA 2 0.0408 Tier II
21 NC_000008.11 8 53921429 + RGS20 NNNACTTGATCTGAGCTGAG NTG 2 0.0278 Tier II
22 NC_000008.11 8 41958806 - KAT6A NNNACATGATCTGAGCTGAT NTG 2 0.0273 Tier II
23 NC_000003.12 3 27468432 + SLC4A7 NNNACATGATCTAAGCTGAG NGC 2 0.0205 Tier II
24 NC_000012.12 12 99035009 + ANKS1B NNNACATGATGTGAGCTGAG NTG 2 0.0097 Tier II
25 NC_000014.9 14 21077760 + ARHGEF40 NNNACATGATCTGAGCTGAG NAT 2 0.0 Tier II
26 NC_000003.12 3 15919258 + LOC107986064 NNNACATGATAAGAGCTGAG NGG 2 0.6 Tier III
27 NC_000020.11 20 23656439 + LOC124904965 NNNACAAGCTCTGAGCTGAG NGG 2 0.5 Tier III
28 NC_000003.12 3 59403012 + CFAP20DC-DT NNNATATGCTCTGAGCTGAG NGG 2 0.3636 Tier III
29 NC_000002.12 2 12256106 - MIR3681HG NNNACAGGAGCTGAGCTGAG NGG 2 0.2941 Tier III
30 NC_000015.10 15 32785785 - LOC107984089 NNNACATGATCTGAGTCGAG NGG 2 0.2172 Tier III
31 NC_000013.11 13 110959015 - LOC105370364 NNNACATGTTCTTAGCTGAG NGG 2 0.18 Tier III
32 NC_000006.12 6 1065296 - LINC01622 NNNCCATGAGCTGAGCTGAG NGG 2 0.1765 Tier III
33 NC_000017.11 17 16443329 + SNHG29 NNNCCATGAGCTGAGCTGAG NGG 2 0.1765 Tier III
34 NC_000003.12 3 18063047 - BALR6 NNNACATGAGCTGAGCTGAG NTG 2 0.0195 Tier III
35 NC_000008.11 8 96430362 - LOC105375653 NNNACATGATCTGAGCCGAG NGC 2 0.0157 Tier III
36 NC_000003.12 3 59685659 + CFAP20DC-DT NNNACATGATCTGAACTGAG NGT 2 0.0152 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 78590926 + Nlk NNNACATGATCCGAGCTGAG NGG 1 0.9474 Tier I
2 NC_000077.6 11 77288843 + Ssh2 NNNATATAATCTGAGCTGAG NGG 2 0.6364 Tier II
3 NC_000072.6 6 88260818 - Eefsec NNNACATCATCTGAGCTGAG NGG 1 0.6154 Tier II
4 NC_000067.6 1 180435084 + Stum NNNAGATGAACTGAGCTGAG NGG 2 0.5143 Tier II
5 NC_000072.6 6 72613321 + Tgoln1 NNNACGTGTTCTGAGCTGAG NGG 2 0.4286 Tier II
6 NC_000083.6 17 62773130 - Efna5 NNNACATGAGCAGAGCTGAG NGG 2 0.4 Tier II
7 NC_000071.6 5 107870469 - Evi5 NNNACATGGTCTGAGCAGAG NGG 2 0.3556 Tier II
8 NC_000069.6 3 104789535 + Rhoc NNNAGCTGATCTGAGCTGAG NGG 2 0.2727 Tier II
9 NC_000070.6 4 15038040 - Necab1 NNNACATGATCTGAGCACAG NGG 2 0.254 Tier II
10 NC_000068.7 2 90704252 - Nup160 NNNACATAATGTGAGCTGAG NGG 2 0.25 Tier II
11 NC_000072.6 6 86208089 - Tgfa NNNACATGCTCTCAGCTGAG NGG 2 0.2406 Tier II
12 NC_000077.6 11 31973397 + 4930524B15Rik NNNACATTATCTTAGCTGAG NGG 2 0.1875 Tier II
13 NC_000081.6 15 92285788 + Cntn1 NNNACATGCTTTGAGCTGAG NGG 2 0.1758 Tier II
14 NC_000068.7 2 141872962 - Macrod2 NNNAGATGATCTGACCTGAG NGG 2 0.1636 Tier II
15 NC_000077.6 11 101086877 - Mlx NNNACATGATCTGATCTAAG NGG 2 0.0989 Tier II
16 NC_000073.6 7 109325426 - Stk33 NNNACATGATCTGAGCTAAG NGA 2 0.0481 Tier II
17 NC_000081.6 15 27909439 + Trio NNNACATGAACTGAGCTGAG NGT 2 0.0138 Tier II
18 NC_000072.6 6 145172125 + Lrmp NNNACATGATGTGAGCTGAG NTG 2 0.0097 Tier II
19 NC_000067.6 1 69961041 + Spag16 NNNACATGAGCTGAGCTGAG NGT 2 0.0081 Tier II
20 NC_000068.7 2 102047156 + Ldlrad3 NNNACATGATCTGATCTGAG NGC 2 0.0032 Tier II
21 NC_000068.7 2 87166643 + Nlk-ps1 NNNACATGATCCGAGCTGAG NGG 1 0.9474 Tier III
22 NC_000076.6 10 72974876 - A330049N07Rik NNNACATGAACTGAGCTGAT NGG 2 0.6 Tier III
23 NC_000073.6 7 130303515 - Gm33555 NNNACACGAGCTGAGCTGAG NGG 2 0.3438 Tier III
24 NC_000073.6 7 144906944 + Gm39119 NNNACATGCCCTGAGCTGAG NGG 2 0.3048 Tier III
25 NC_000079.6 13 53638156 - 9530014B07Rik NNNACATGATCAGAGTTGAG NGG 2 0.2462 Tier III
26 NC_000069.6 3 104569590 + Gm34490 NNNACATGATATGAGCTGAG NAG 2 0.1944 Tier III
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Other clones with same target sequence:

(none)