Construct: sgRNA BRDN0001147680
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGACATGATCTGAGCTGAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NLK (51701)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000017.11 | 17 | 28161224 | - | NLK | NNNACATGATCTGAGCTGAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000003.12 | 3 | 161232752 | - | NMD3 | NNNAGATGATCTGAACTGAG | NGG | 2 | 0.5647 | Tier II |
3 | NC_000005.10 | 5 | 170522508 | + | KCNIP1 | NNNACATGATCTGAGCTAAT | NGG | 2 | 0.4846 | Tier II |
4 | NC_000022.11 | 22 | 30975471 | - | TUG1 | NNNACATGAACTGTGCTGAG | NGG | 2 | 0.4571 | Tier II |
5 | NC_000020.11 | 20 | 63282967 | + | ARFGAP1 | NNNACATGCTCAGAGCTGAG | NGG | 2 | 0.4571 | Tier II |
6 | NC_000006.12 | 6 | 98899966 | - | FBXL4 | NNNACATTTTCTGAGCTGAG | NGG | 2 | 0.375 | Tier II |
7 | NC_000007.14 | 7 | 40395656 | - | SUGCT | NNNACATTTTCTGAGCTGAG | NGG | 2 | 0.375 | Tier II |
8 | NC_000020.11 | 20 | 4173817 | + | SMOX | NNNACAGCATCTGAGCTGAG | NGG | 2 | 0.362 | Tier II |
9 | NC_000011.10 | 11 | 61729156 | - | DAGLA | NNNGCATGATCTGAGCAGAG | NGG | 2 | 0.3333 | Tier II |
10 | NC_000006.12 | 6 | 108870371 | + | ARMC2 | NNNGCATGATCGGAGCTGAG | NGG | 2 | 0.3125 | Tier II |
11 | NC_000007.14 | 7 | 88866704 | - | ZNF804B | NNNAGATGAGCTGAGCTGAG | NGG | 2 | 0.3 | Tier II |
12 | NC_000004.12 | 4 | 6432833 | + | PPP2R2C | NNNACATGAGCTGAGCTGTG | NGG | 2 | 0.2692 | Tier II |
13 | NC_000022.11 | 22 | 32821604 | + | TIMP3 | NNNAGATGATCTCAGCTGAG | NGG | 2 | 0.2526 | Tier II |
14 | NC_000022.11 | 22 | 32821604 | + | SYN3 | NNNAGATGATCTCAGCTGAG | NGG | 2 | 0.2526 | Tier II |
15 | NC_000015.10 | 15 | 32785785 | - | FMN1 | NNNACATGATCTGAGTCGAG | NGG | 2 | 0.2172 | Tier II |
16 | NC_000017.11 | 17 | 16443329 | + | LRRC75A | NNNCCATGAGCTGAGCTGAG | NGG | 2 | 0.1765 | Tier II |
17 | NC_000006.12 | 6 | 4779822 | - | CDYL | NNNACATGAGGTGAGCTGAG | NGG | 2 | 0.125 | Tier II |
18 | NC_000001.11 | 1 | 167769001 | + | MPZL1 | NNNACATGATCTCACCTGAG | NGG | 2 | 0.1148 | Tier II |
19 | NC_000002.12 | 2 | 74093487 | - | TET3 | NNNACATGACCTGAGGTGAG | NGG | 2 | 0.0821 | Tier II |
20 | NC_000003.12 | 3 | 54334877 | - | CACNA2D3 | NNNACAGGATCTGAGCTGAG | NGA | 2 | 0.0408 | Tier II |
21 | NC_000008.11 | 8 | 53921429 | + | RGS20 | NNNACTTGATCTGAGCTGAG | NTG | 2 | 0.0278 | Tier II |
22 | NC_000008.11 | 8 | 41958806 | - | KAT6A | NNNACATGATCTGAGCTGAT | NTG | 2 | 0.0273 | Tier II |
23 | NC_000003.12 | 3 | 27468432 | + | SLC4A7 | NNNACATGATCTAAGCTGAG | NGC | 2 | 0.0205 | Tier II |
24 | NC_000012.12 | 12 | 99035009 | + | ANKS1B | NNNACATGATGTGAGCTGAG | NTG | 2 | 0.0097 | Tier II |
25 | NC_000014.9 | 14 | 21077760 | + | ARHGEF40 | NNNACATGATCTGAGCTGAG | NAT | 2 | 0.0 | Tier II |
26 | NC_000003.12 | 3 | 15919258 | + | LOC107986064 | NNNACATGATAAGAGCTGAG | NGG | 2 | 0.6 | Tier III |
27 | NC_000020.11 | 20 | 23656439 | + | LOC124904965 | NNNACAAGCTCTGAGCTGAG | NGG | 2 | 0.5 | Tier III |
28 | NC_000003.12 | 3 | 59403012 | + | CFAP20DC-DT | NNNATATGCTCTGAGCTGAG | NGG | 2 | 0.3636 | Tier III |
29 | NC_000002.12 | 2 | 12256106 | - | MIR3681HG | NNNACAGGAGCTGAGCTGAG | NGG | 2 | 0.2941 | Tier III |
30 | NC_000015.10 | 15 | 32785785 | - | LOC107984089 | NNNACATGATCTGAGTCGAG | NGG | 2 | 0.2172 | Tier III |
31 | NC_000013.11 | 13 | 110959015 | - | LOC105370364 | NNNACATGTTCTTAGCTGAG | NGG | 2 | 0.18 | Tier III |
32 | NC_000006.12 | 6 | 1065296 | - | LINC01622 | NNNCCATGAGCTGAGCTGAG | NGG | 2 | 0.1765 | Tier III |
33 | NC_000017.11 | 17 | 16443329 | + | SNHG29 | NNNCCATGAGCTGAGCTGAG | NGG | 2 | 0.1765 | Tier III |
34 | NC_000003.12 | 3 | 18063047 | - | BALR6 | NNNACATGAGCTGAGCTGAG | NTG | 2 | 0.0195 | Tier III |
35 | NC_000008.11 | 8 | 96430362 | - | LOC105375653 | NNNACATGATCTGAGCCGAG | NGC | 2 | 0.0157 | Tier III |
36 | NC_000003.12 | 3 | 59685659 | + | CFAP20DC-DT | NNNACATGATCTGAACTGAG | NGT | 2 | 0.0152 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000077.6 | 11 | 78590926 | + | Nlk | NNNACATGATCCGAGCTGAG | NGG | 1 | 0.9474 | Tier I |
2 | NC_000077.6 | 11 | 77288843 | + | Ssh2 | NNNATATAATCTGAGCTGAG | NGG | 2 | 0.6364 | Tier II |
3 | NC_000072.6 | 6 | 88260818 | - | Eefsec | NNNACATCATCTGAGCTGAG | NGG | 1 | 0.6154 | Tier II |
4 | NC_000067.6 | 1 | 180435084 | + | Stum | NNNAGATGAACTGAGCTGAG | NGG | 2 | 0.5143 | Tier II |
5 | NC_000072.6 | 6 | 72613321 | + | Tgoln1 | NNNACGTGTTCTGAGCTGAG | NGG | 2 | 0.4286 | Tier II |
6 | NC_000083.6 | 17 | 62773130 | - | Efna5 | NNNACATGAGCAGAGCTGAG | NGG | 2 | 0.4 | Tier II |
7 | NC_000071.6 | 5 | 107870469 | - | Evi5 | NNNACATGGTCTGAGCAGAG | NGG | 2 | 0.3556 | Tier II |
8 | NC_000069.6 | 3 | 104789535 | + | Rhoc | NNNAGCTGATCTGAGCTGAG | NGG | 2 | 0.2727 | Tier II |
9 | NC_000070.6 | 4 | 15038040 | - | Necab1 | NNNACATGATCTGAGCACAG | NGG | 2 | 0.254 | Tier II |
10 | NC_000068.7 | 2 | 90704252 | - | Nup160 | NNNACATAATGTGAGCTGAG | NGG | 2 | 0.25 | Tier II |
11 | NC_000072.6 | 6 | 86208089 | - | Tgfa | NNNACATGCTCTCAGCTGAG | NGG | 2 | 0.2406 | Tier II |
12 | NC_000077.6 | 11 | 31973397 | + | 4930524B15Rik | NNNACATTATCTTAGCTGAG | NGG | 2 | 0.1875 | Tier II |
13 | NC_000081.6 | 15 | 92285788 | + | Cntn1 | NNNACATGCTTTGAGCTGAG | NGG | 2 | 0.1758 | Tier II |
14 | NC_000068.7 | 2 | 141872962 | - | Macrod2 | NNNAGATGATCTGACCTGAG | NGG | 2 | 0.1636 | Tier II |
15 | NC_000077.6 | 11 | 101086877 | - | Mlx | NNNACATGATCTGATCTAAG | NGG | 2 | 0.0989 | Tier II |
16 | NC_000073.6 | 7 | 109325426 | - | Stk33 | NNNACATGATCTGAGCTAAG | NGA | 2 | 0.0481 | Tier II |
17 | NC_000081.6 | 15 | 27909439 | + | Trio | NNNACATGAACTGAGCTGAG | NGT | 2 | 0.0138 | Tier II |
18 | NC_000072.6 | 6 | 145172125 | + | Lrmp | NNNACATGATGTGAGCTGAG | NTG | 2 | 0.0097 | Tier II |
19 | NC_000067.6 | 1 | 69961041 | + | Spag16 | NNNACATGAGCTGAGCTGAG | NGT | 2 | 0.0081 | Tier II |
20 | NC_000068.7 | 2 | 102047156 | + | Ldlrad3 | NNNACATGATCTGATCTGAG | NGC | 2 | 0.0032 | Tier II |
21 | NC_000068.7 | 2 | 87166643 | + | Nlk-ps1 | NNNACATGATCCGAGCTGAG | NGG | 1 | 0.9474 | Tier III |
22 | NC_000076.6 | 10 | 72974876 | - | A330049N07Rik | NNNACATGAACTGAGCTGAT | NGG | 2 | 0.6 | Tier III |
23 | NC_000073.6 | 7 | 130303515 | - | Gm33555 | NNNACACGAGCTGAGCTGAG | NGG | 2 | 0.3438 | Tier III |
24 | NC_000073.6 | 7 | 144906944 | + | Gm39119 | NNNACATGCCCTGAGCTGAG | NGG | 2 | 0.3048 | Tier III |
25 | NC_000079.6 | 13 | 53638156 | - | 9530014B07Rik | NNNACATGATCAGAGTTGAG | NGG | 2 | 0.2462 | Tier III |
26 | NC_000069.6 | 3 | 104569590 | + | Gm34490 | NNNACATGATATGAGCTGAG | NAG | 2 | 0.1944 | Tier III |