Construct: sgRNA BRDN0001147684

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATACTTAGCTGTAACACGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSKH1 (5681)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76740

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 67909028 - PSKH1 NNNACTTAGCTGTAACACGT NGG 0 1.0 Tier I
2 NC_000001.11 1 151199180 - PIP5K1A NNNACTTACCGGTAACACGT NGG 2 0.2154 Tier I
3 NC_000013.11 13 79366641 + RBM26 NNNACTTACCTGTAACAGGT NGG 2 0.0718 Tier I
4 NC_000008.11 8 42167517 + AP3M2 NNNACTTAACAGTAACACGT NGG 2 0.4821 Tier II
5 NC_000003.12 3 126076785 - SLC41A3 NNNACTCAGCTGTAACACTT NGG 2 0.4583 Tier II
6 NC_000007.14 7 27882402 - JAZF1 NNNATTTAGCTGTAACAAGT NGG 2 0.3427 Tier II
7 NC_000013.11 13 29493517 + MTUS2 NNNCCATAGCTGTAACACGT NGG 2 0.3059 Tier II
8 NC_000008.11 8 49959983 - SNTG1 NNNAATTAGCTGTAACACCT NGG 2 0.2562 Tier II
9 NC_000008.11 8 25286202 - DOCK5 NNNACGTAGCTGTAACACCT NGG 2 0.2562 Tier II
10 NC_000023.11 X 53629477 - HUWE1 NNNCCTTAGCTGTAACACTT NGG 2 0.2353 Tier II
11 NC_000009.12 9 123588742 - DENND1A NNNACTTTGCTTTAACACGT NGG 2 0.1648 Tier II
12 NC_000015.10 15 40293886 - PLCB2 NNNGCTTAGCTGTAACCCGT NGG 2 0.1103 Tier II
13 NC_000005.10 5 96554434 + CAST NNNACTTAACTGTAAGACGT NGG 2 0.0989 Tier II
14 NC_000007.14 7 16612153 - ANKMY2 NNNATTTAGCTGTAACAGGT NGG 2 0.0848 Tier II
15 NC_000016.10 16 63488138 - LOC105371308 NNNACTTAGCTGTATCACAT NGG 2 0.1429 Tier III
16 NC_000005.10 5 96554434 + LOC101929710 NNNACTTAACTGTAAGACGT NGG 2 0.0989 Tier III
17 NC_000007.14 7 16612153 - LOC105375169 NNNATTTAGCTGTAACAGGT NGG 2 0.0848 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 105912968 - Pskh1 NNNACTTAGCTGTCACTCGT NGG 2 0.0286 Tier I
2 NC_000084.6 18 67092958 - Gnal NNNACTGAGCTGTAACACGT NGG 1 0.5882 Tier II
3 NC_000068.7 2 163318493 + Tox2 NNNAATTAGCTGCAACACGT NGG 2 0.4511 Tier II
4 NC_000067.6 1 193007860 + Syt14 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
5 NC_000070.6 4 57211839 + Ptpn3 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
6 NC_000071.6 5 43888349 - Cd38 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
7 NC_000071.6 5 122659527 - P2rx7 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
8 NC_000074.6 8 73201384 + Large1 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
9 NC_000083.6 17 7205896 - Rps6ka2 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
10 NC_000083.6 17 42778247 - Cd2ap NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
11 NC_000085.6 19 20530605 - Aldh1a1 NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier II
12 NC_000071.6 5 142709826 + Slc29a4 NNNCCTTAGCTGTAACACGT NGG 1 0.3529 Tier II
13 NC_000086.7 X 36893099 - Nkrf NNNACTTAGCTGTAACGCCT NGG 2 0.0791 Tier II
14 NC_000076.6 10 111332165 - Gm40761 NNNACTGAGCTGTAACACAT NGG 2 0.4202 Tier III
15 NC_000076.6 10 42022661 + 1700016J18Rik NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier III
16 NC_000085.6 19 20530605 - E030003E18Rik NNNACTGAGCTGTAACACTT NGG 2 0.3922 Tier III
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Other clones with same target sequence:

(none)