Construct: sgRNA BRDN0001147684
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATACTTAGCTGTAACACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PSKH1 (5681)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76740
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000016.10 |
16 |
67909028 |
- |
PSKH1 |
NNNACTTAGCTGTAACACGT |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000001.11 |
1 |
151199180 |
- |
PIP5K1A |
NNNACTTACCGGTAACACGT |
NGG |
2 |
0.2154 |
Tier I |
3 |
NC_000013.11 |
13 |
79366641 |
+ |
RBM26 |
NNNACTTACCTGTAACAGGT |
NGG |
2 |
0.0718 |
Tier I |
4 |
NC_000008.11 |
8 |
42167517 |
+ |
AP3M2 |
NNNACTTAACAGTAACACGT |
NGG |
2 |
0.4821 |
Tier II |
5 |
NC_000003.12 |
3 |
126076785 |
- |
SLC41A3 |
NNNACTCAGCTGTAACACTT |
NGG |
2 |
0.4583 |
Tier II |
6 |
NC_000007.14 |
7 |
27882402 |
- |
JAZF1 |
NNNATTTAGCTGTAACAAGT |
NGG |
2 |
0.3427 |
Tier II |
7 |
NC_000013.11 |
13 |
29493517 |
+ |
MTUS2 |
NNNCCATAGCTGTAACACGT |
NGG |
2 |
0.3059 |
Tier II |
8 |
NC_000008.11 |
8 |
49959983 |
- |
SNTG1 |
NNNAATTAGCTGTAACACCT |
NGG |
2 |
0.2562 |
Tier II |
9 |
NC_000008.11 |
8 |
25286202 |
- |
DOCK5 |
NNNACGTAGCTGTAACACCT |
NGG |
2 |
0.2562 |
Tier II |
10 |
NC_000023.11 |
X |
53629477 |
- |
HUWE1 |
NNNCCTTAGCTGTAACACTT |
NGG |
2 |
0.2353 |
Tier II |
11 |
NC_000009.12 |
9 |
123588742 |
- |
DENND1A |
NNNACTTTGCTTTAACACGT |
NGG |
2 |
0.1648 |
Tier II |
12 |
NC_000015.10 |
15 |
40293886 |
- |
PLCB2 |
NNNGCTTAGCTGTAACCCGT |
NGG |
2 |
0.1103 |
Tier II |
13 |
NC_000005.10 |
5 |
96554434 |
+ |
CAST |
NNNACTTAACTGTAAGACGT |
NGG |
2 |
0.0989 |
Tier II |
14 |
NC_000007.14 |
7 |
16612153 |
- |
ANKMY2 |
NNNATTTAGCTGTAACAGGT |
NGG |
2 |
0.0848 |
Tier II |
15 |
NC_000016.10 |
16 |
63488138 |
- |
LOC105371308 |
NNNACTTAGCTGTATCACAT |
NGG |
2 |
0.1429 |
Tier III |
16 |
NC_000005.10 |
5 |
96554434 |
+ |
LOC101929710 |
NNNACTTAACTGTAAGACGT |
NGG |
2 |
0.0989 |
Tier III |
17 |
NC_000007.14 |
7 |
16612153 |
- |
LOC105375169 |
NNNATTTAGCTGTAACAGGT |
NGG |
2 |
0.0848 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000074.6 |
8 |
105912968 |
- |
Pskh1 |
NNNACTTAGCTGTCACTCGT |
NGG |
2 |
0.0286 |
Tier I |
2 |
NC_000084.6 |
18 |
67092958 |
- |
Gnal |
NNNACTGAGCTGTAACACGT |
NGG |
1 |
0.5882 |
Tier II |
3 |
NC_000068.7 |
2 |
163318493 |
+ |
Tox2 |
NNNAATTAGCTGCAACACGT |
NGG |
2 |
0.4511 |
Tier II |
4 |
NC_000067.6 |
1 |
193007860 |
+ |
Syt14 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
5 |
NC_000070.6 |
4 |
57211839 |
+ |
Ptpn3 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
6 |
NC_000071.6 |
5 |
43888349 |
- |
Cd38 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
7 |
NC_000071.6 |
5 |
122659527 |
- |
P2rx7 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
8 |
NC_000074.6 |
8 |
73201384 |
+ |
Large1 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
9 |
NC_000083.6 |
17 |
7205896 |
- |
Rps6ka2 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
10 |
NC_000083.6 |
17 |
42778247 |
- |
Cd2ap |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
11 |
NC_000085.6 |
19 |
20530605 |
- |
Aldh1a1 |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier II |
12 |
NC_000071.6 |
5 |
142709826 |
+ |
Slc29a4 |
NNNCCTTAGCTGTAACACGT |
NGG |
1 |
0.3529 |
Tier II |
13 |
NC_000086.7 |
X |
36893099 |
- |
Nkrf |
NNNACTTAGCTGTAACGCCT |
NGG |
2 |
0.0791 |
Tier II |
14 |
NC_000076.6 |
10 |
111332165 |
- |
Gm40761 |
NNNACTGAGCTGTAACACAT |
NGG |
2 |
0.4202 |
Tier III |
15 |
NC_000076.6 |
10 |
42022661 |
+ |
1700016J18Rik |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier III |
16 |
NC_000085.6 |
19 |
20530605 |
- |
E030003E18Rik |
NNNACTGAGCTGTAACACTT |
NGG |
2 |
0.3922 |
Tier III |
Other clones with same target sequence:
(none)