Construct: sgRNA BRDN0001147688
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGCTACACACCTGACATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TESK2 (10420)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76434
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
45347924 |
+ |
TESK2 |
NNNCTACACACCTGACATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
78658514 |
+ |
WWOX |
NNNCCAGACACCTGACATCG |
NGG |
2 |
0.4706 |
Tier II |
| 3 |
NC_000001.11 |
1 |
156598595 |
+ |
GPATCH4 |
NNNCCACACACCTGACATTG |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000017.11 |
17 |
56889997 |
- |
TRIM25 |
NNNCTACACACCTGACATGC |
NGG |
2 |
0.0536 |
Tier II |
| 5 |
NC_000015.10 |
15 |
73906869 |
- |
TBC1D21 |
NNNCTACACACATGACATCG |
NTG |
2 |
0.0278 |
Tier II |
| 6 |
NC_000022.11 |
22 |
29419161 |
+ |
RFPL1 |
NNNCTACACACCTGACATCT |
NTG |
2 |
0.0273 |
Tier II |
| 7 |
NC_000013.11 |
13 |
60222814 |
- |
LINC00434 |
NNNCTACCCACCTGACATCG |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
29849389 |
+ |
Myt1l |
NNNCTACACACATGACATGG |
NGG |
2 |
0.0893 |
Tier I |
| 2 |
NC_000068.7 |
2 |
153139483 |
+ |
Hck |
NNNCTACAGACCTGAAATCG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000070.6 |
4 |
128940261 |
+ |
Azin2 |
NNNCTACACACTTGACATCA |
NGG |
2 |
0.5048 |
Tier II |
| 4 |
NC_000077.6 |
11 |
36697090 |
- |
Tenm2 |
NNNCTTCAGACCTGACATCG |
NGG |
2 |
0.4422 |
Tier II |
| 5 |
NC_000073.6 |
7 |
139407759 |
- |
Inpp5a |
NNNCTACACACCTGACATCC |
NGG |
1 |
0.4286 |
Tier II |
| 6 |
NC_000084.6 |
18 |
82962431 |
- |
Zfp516 |
NNNCTACACACCTGACACCA |
NGG |
2 |
0.4018 |
Tier II |
| 7 |
NC_000075.6 |
9 |
78582275 |
+ |
Slc17a5 |
NNNCTACACACCTGACAGCA |
NGG |
2 |
0.3125 |
Tier II |
| 8 |
NC_000068.7 |
2 |
49720068 |
+ |
Kif5c |
NNNCTAGACACCGGACATCG |
NGG |
2 |
0.1228 |
Tier II |
| 9 |
NC_000074.6 |
8 |
124332803 |
+ |
Galnt2 |
NNNCTACACACCTGACCACG |
NGG |
2 |
0.1176 |
Tier II |
Other clones with same target sequence:
(none)
