Construct: sgRNA BRDN0001147695

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCCTCAAGTCCACAGACGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AATK (9625)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77316

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 81128528 - AATK NNNCTCAAGTCCACAGACGT NGG 0 1.0 Tier I
2 NC_000001.11 1 42591303 + CCDC30 NNNCACAGGTCCACAGACGT NGG 2 0.3667 Tier II
3 NC_000010.11 10 47581260 + ANXA8 NNNCTCCAGTCCACAGATGT NGG 2 0.2812 Tier II
4 NC_000002.12 2 205031026 - PARD3B NNNCTCATGTCCACAGACCT NGG 2 0.1921 Tier II
5 NC_000006.12 6 38501702 - BTBD9 NNNCTCAAGTGCACAGACAT NGG 2 0.1786 Tier II
6 NC_000023.11 X 26138679 + MAGEB18 NNNCTCAAGTCAACAGACGG NGG 2 0.1261 Tier II
7 NC_000017.11 17 269857 + RPH3AL NNNCTCCAGTGCACAGACGT NGG 2 0.1094 Tier II
8 NC_000016.10 16 12385296 - SNX29 NNNCACAAGTCCCCAGACGT NGG 2 0.1053 Tier II
9 NC_000011.10 11 132101152 - NTM NNNCTCAAGTCCATAGAGGT NGG 2 0.0978 Tier II
10 NC_000002.12 2 73203577 - NOTO NNNCTCAAGTTCACAGACGT NAG 2 0.0798 Tier II
11 NC_000006.12 6 108967956 + ARMC2 NNNCTCAAGCCCACAGTCGT NGG 2 0.0711 Tier II
12 NC_000011.10 11 133141123 - OPCML NNNCTCAAGTCCACACACTT NGG 2 0.0 Tier II
13 NC_000010.11 10 19330754 + MALRD1 NNNCTGAAGTCCACACACGT NGG 2 0.0 Tier II
14 NC_000001.11 1 42591303 + LOC124904162 NNNCACAGGTCCACAGACGT NGG 2 0.3667 Tier III
15 NC_000010.11 10 46759153 - GLUD1P2 NNNCTCCAGTCCACAGATGT NGG 2 0.2812 Tier III
16 NC_000010.11 10 47581260 + CTSLP2 NNNCTCCAGTCCACAGATGT NGG 2 0.2812 Tier III
17 NC_000010.11 10 50707838 + CTSLP4 NNNCTCCAGTCCACAGATGT NGG 2 0.2812 Tier III
18 NC_000010.11 10 79872835 + CTSLP6 NNNCTCCAGTCCACAGATGT NGG 2 0.2812 Tier III
19 NC_000010.11 10 87389137 + CTSLP1 NNNCTCCAGTCCACAGATGT NGG 2 0.2812 Tier III
20 NC_000002.12 2 12542509 - MIR3681HG NNNCTCAAGACCACAGACGT NAG 2 0.2222 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 64367908 + Ctsl NNNCTCCAGTCCACAGACTT NGG 2 0.2917 Tier I
2 NC_000077.6 11 120016786 - Aatk NNNCTCAAGTCCACGGACCT NGG 2 0.2914 Tier I
3 NC_000081.6 15 42494623 - Angpt1 NNNCTTAAGTACACAGACGT NGG 2 0.6964 Tier II
4 NC_000078.6 12 19537041 - Gm6943 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
5 NC_000078.6 12 19846441 + Gm38702 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
6 NC_000078.6 12 19993699 - Gm40853 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
7 NC_000078.6 12 20122220 - Gm9265 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
8 NC_000078.6 12 22688058 + Gm9295 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
9 NC_000078.6 12 24033820 + Gm5954 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
10 NC_000078.6 12 24317368 + Gm4120 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier II
11 NC_000078.6 12 17777894 - Hpcal1 NNNCTCAGGTCCACAGACCT NGG 2 0.3287 Tier II
12 NC_000078.6 12 18890038 - Gm40847 NNNCTCAGGTCCACAGACCT NGG 2 0.3287 Tier II
13 NC_000078.6 12 22430850 + Gm10476 NNNCTCAGGTCCACAGACCT NGG 2 0.3287 Tier II
14 NC_000078.6 12 23589880 + Gm40862 NNNCTCAGGTCCACAGACCT NGG 2 0.3287 Tier II
15 NC_000068.7 2 173155444 - Pck1 NNNCTCAAGTCCCCAGATGT NGG 2 0.1353 Tier II
16 NC_000071.6 5 136843826 + Col26a1 NNNCTCAGGTCCACAGAGGT NGG 2 0.0978 Tier II
17 NC_000071.6 5 75617699 - Kit NNNCTCAAGTCCACAGACTT NGA 2 0.0463 Tier II
18 NC_000075.6 9 67849609 - Vps13c NNNCTCAAGTCCACAGACAT NGC 2 0.0159 Tier II
19 NC_000075.6 9 86372665 - Ube2cbp NNNCTCAAGTCCTCAGACGT NGC 2 0.0067 Tier II
20 NC_000078.6 12 18170591 - LOC108167983 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier III
21 NC_000078.6 12 20536120 - Gm4012 NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier III
22 NC_000078.6 12 22969829 + 2410018L13Rik NNNCTCAGGTCCACAGACAT NGG 2 0.5238 Tier III
23 NC_000073.6 7 30996052 - Gm42377 NNNCTCAAGTGCACACACGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)