Construct: sgRNA BRDN0001147696
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAGATGGATGATCGTCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K1 (4214)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77825
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
56856656 |
+ |
MAP3K1 |
NNNGATGGATGATCGTCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
31278259 |
- |
DMD |
NNNGATGCATGATTGTCCAG |
NGG |
2 |
0.4513 |
Tier II |
| 3 |
NC_000023.11 |
X |
64214753 |
+ |
LOC112268307 |
NNNGCTGGCTGATCGTCCAG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000008.11 |
8 |
141429355 |
+ |
PTP4A3 |
NNNGATGGATGAGCCTCCAG |
NGG |
2 |
0.0711 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
137854895 |
- |
Pilrb1 |
NNNGATGGATGATCTTCCTG |
NGG |
2 |
0.0769 |
Tier I |
| 2 |
NC_000071.6 |
5 |
137868595 |
- |
Pilrb2 |
NNNGATGGATGATCTTCCTG |
NGG |
2 |
0.0769 |
Tier I |
| 3 |
NC_000068.7 |
2 |
179565138 |
+ |
Cdh4 |
NNNGATGGATGATCGATCAG |
NGG |
2 |
0.4242 |
Tier II |
| 4 |
NC_000073.6 |
7 |
101482312 |
+ |
Pde2a |
NNNGATGGATGACCCTCCAG |
NGG |
2 |
0.2153 |
Tier II |
| 5 |
NC_000077.6 |
11 |
104634525 |
- |
Itgb3 |
NNNGATGGGTGATCTTCCAG |
NGG |
2 |
0.0952 |
Tier II |
| 6 |
NC_000082.6 |
16 |
32809730 |
+ |
Smbd1 |
NNNGTTGGATGATCTTCCAG |
NGG |
2 |
0.0519 |
Tier II |
| 7 |
NC_000086.7 |
X |
138211116 |
+ |
Il1rapl2 |
NNNGATGGATGTTCTTCCAG |
NGG |
2 |
0.0476 |
Tier II |
| 8 |
NC_000083.6 |
17 |
46605742 |
+ |
Ptk7 |
NNNGATGGATGCTCTTCCAG |
NGG |
2 |
0.0376 |
Tier II |
| 9 |
NC_000067.6 |
1 |
175670005 |
- |
Opn3 |
NNNGATGGATGATGGTCCAG |
NGC |
2 |
0.0 |
Tier II |
| 10 |
NC_000068.7 |
2 |
167986233 |
+ |
Ripor3 |
NNNGATGGATGGTGGTCCAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000082.6 |
16 |
90839109 |
- |
Eva1c |
NNNGATGGGTGATGGTCCAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000082.6 |
16 |
90839109 |
- |
Gm36363 |
NNNGATGGGTGATGGTCCAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
