Construct: sgRNA BRDN0001147698

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAAACTTCAGCCAACTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK9 (5601)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76718

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 180242679 - MAPK9 NNNAACTTCAGCCAACTGTG NGG 0 1.0 Tier I
2 NC_000001.11 1 39189461 - MACF1 NNNAACTTCTGCCAACTTTG NGG 2 0.5882 Tier II
3 NC_000005.10 5 73885210 - ARHGEF28 NNNAACTTAAGCCAGCTGTG NGG 2 0.5571 Tier II
4 NC_000012.12 12 85180217 + LRRIQ1 NNNAAGTTCAACCAACTGTG NGG 2 0.5 Tier II
5 NC_000016.10 16 10117171 - GRIN2A NNNAACTAGAGCCAACTGTG NGG 2 0.4952 Tier II
6 NC_000006.12 6 151862395 - ESR1 NNNAACTCCAGCCAACTCTG NGG 2 0.4762 Tier II
7 NC_000007.14 7 38872181 - VPS41 NNNAACTTCTGCCAACAGTG NGG 2 0.4706 Tier II
8 NC_000009.12 9 133544708 + ADAMTSL2 NNNAACTTGAGCCAGCTGTG NGG 2 0.4024 Tier II
9 NC_000006.12 6 82920135 - UBE3D NNNAGCTTCAGTCAACTGTG NGG 2 0.3877 Tier II
10 NC_000004.12 4 183924272 - STOX2 NNNATCTCCAGCCAACTGTG NGG 2 0.3636 Tier II
11 NC_000015.10 15 41818806 + MAPKBP1 NNNTCCTTCAGCCAACTGTG NGG 2 0.3182 Tier II
12 NC_000009.12 9 130611079 - FUBP3 NNNCACTTTAGCCAACTGTG NGG 2 0.3088 Tier II
13 NC_000002.12 2 111000251 - ACOXL NNNAACTTCAGGCAACAGTG NGG 2 0.237 Tier II
14 NC_000001.11 1 214611841 - CENPF NNNAACTTCAGCCAACTCTC NGG 2 0.2041 Tier II
15 NC_000004.12 4 97856521 - STPG2 NNNAACTTCAGCAAACTCTG NGG 2 0.1832 Tier II
16 NC_000004.12 4 37498690 - PGCKA1 NNNAACTTCAGCAAACTGTC NGG 2 0.1648 Tier II
17 NC_000011.10 11 62389847 - ASRGL1 NNNAACTTCAGTCACCTGTG NGG 2 0.1469 Tier II
18 NC_000020.11 20 2876354 - PTPRA NNNAACTTCAGCCATCTCTG NGG 2 0.0952 Tier II
19 NC_000002.12 2 76932990 - LRRTM4 NNNAACTTCAACCAACTGTG NGA 2 0.0694 Tier II
20 NC_000002.12 2 239361282 - HDAC4 NNNAACTTCAGCCCCCTGTG NGG 2 0.0584 Tier II
21 NC_000023.11 X 96973068 - DIAPH2 NNNAACTTCAGCCAACTGTA NGC 2 0.0208 Tier II
22 NC_000015.10 15 71031883 + LRRC49 NNNAACTTCAGCCTACTGTG NTG 2 0.0208 Tier II
23 NC_000019.10 19 41276139 + HNRNPUL1 NNNAACATCAGCCAACTGTG NGC 2 0.0194 Tier II
24 NC_000010.11 10 66783942 + CTNNA3 NNNAACTTCAGCCAACAGTG NGT 2 0.0086 Tier II
25 NC_000010.11 10 132865826 + CFAP46 NNNAACTTCAGCAAACTGTG NGT 2 0.0062 Tier II
26 NC_000011.10 11 99591049 + CNTN5 NNNAACTTCAGCCCACTGTG NGT 2 0.0035 Tier II
27 NC_000024.10 Y 21293610 - LOC107987347 NNNAATTTCTGCCAACTGTG NGG 2 0.8193 Tier III
28 NC_000005.10 5 66401381 - LOC105379003 NNNAACTTCAGCAAACTGTC NGG 2 0.1648 Tier III
29 NC_000021.9 21 29256195 - LINC00189 NNNAATTTCAGCCAAGTGTG NGG 2 0.1429 Tier III
30 NC_000001.11 1 117140092 - LOC124904387 NNNATCTTCAGCCATCTGTG NGG 2 0.0727 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 49878308 + Mapk9 NNNAACTTCAGCCAACTGTA NGG 1 0.9375 Tier I
2 NC_000072.6 6 124850652 - P3h3 NNNAACTTCAGCCGAGTGTG NGG 2 0.0718 Tier I
3 NC_000069.6 3 95194802 + Gabpb2 NNNGACTTCAGCCAACTGTG NGG 1 0.625 Tier II
4 NC_000068.7 2 53037080 - Fmnl2 NNNAGCTTAAGCCAACTGTG NGG 2 0.6171 Tier II
5 NC_000068.7 2 181197588 + Ptk6 NNNAACCTCAGCTAACTGTG NGG 2 0.4812 Tier II
6 NC_000085.6 19 34819688 + Pank1 NNNCACTCCAGCCAACTGTG NGG 2 0.3529 Tier II
7 NC_000076.6 10 59704476 - Micu1 NNNATATTCAGCCAACTGTG NGG 2 0.3377 Tier II
8 NC_000077.6 11 51973360 + Cdkn2aipnl NNNAACTTCAGCCAGCTCTG NGG 2 0.3095 Tier II
9 NC_000078.6 12 81090495 - Smoc1 NNNAACTTCACACAACTGTG NGG 2 0.3061 Tier II
10 NC_000074.6 8 45008633 + Fat1 NNNAACCTCAGGCAACTGTG NGG 2 0.3056 Tier II
11 NC_000076.6 10 86701518 + Hsp90b1 NNNAACTGCAGCCAACTGTG NAG 2 0.1901 Tier II
12 NC_000073.6 7 139898749 - Kndc1 NNNATCTTCAGCCAACTCTG NGG 2 0.1732 Tier II
13 NC_000071.6 5 128386377 - Tmem132d NNNGACTTCAGCCAACTGTG NAG 2 0.162 Tier II
14 NC_000071.6 5 142438138 - Foxk1 NNNAACTCCAGCCAAGTGTG NGG 2 0.1538 Tier II
15 NC_000077.6 11 88619692 - Msi2 NNNCACTTCCGCCAACTGTG NGG 2 0.1176 Tier II
16 NC_000079.6 13 73494509 + Lpcat1 NNNAAGTTCAGCCCACTGTG NGG 2 0.1071 Tier II
17 NC_000074.6 8 16772627 - Csmd1 NNNACCTTCAGCCATCTGTG NGG 2 0.1 Tier II
18 NC_000068.7 2 18962971 - Pip4k2a NNNAAGTTCAGCCAAGTGTG NGG 2 0.0769 Tier II
19 NC_000067.6 1 40812494 + Tmem182 NNNAACATCAGCCAACTGTG NGT 2 0.0141 Tier II
20 NC_000077.6 11 23273803 - Xpo1 NNNAACTTCAGCCAAGTGTG NTG 2 0.006 Tier II
21 NC_000084.6 18 34365340 + Reep5 NNNAACTTCAGCCAAGTGTG NTG 2 0.006 Tier II
22 NC_000078.6 12 115041705 - Igh NNNAACTTCAGCCAACTGTT NAG 2 0.1815 Tier III
23 NC_000077.6 11 88619692 - 0610039H22Rik NNNCACTTCCGCCAACTGTG NGG 2 0.1176 Tier III
24 NC_000083.6 17 45722111 + Gm35456 NNNAACTTCAGCCAACTGGG NAG 2 0.0648 Tier III
25 NC_000077.6 11 86012107 + Gm20759 NNNAACTTCAGCCAAGTGTG NTG 2 0.006 Tier III
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Other clones with same target sequence:

(none)