Construct: sgRNA BRDN0001147700
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCTCTCGGGCAGACAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCD (5580)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76003
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
53185931 |
+ |
PRKCD |
NNNTCTCGGGCAGACAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
48823530 |
- |
AGBL4 |
NNNTCTCAGGCAGACAACAA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000003.12 |
3 |
64132964 |
+ |
PRICKLE2 |
NNNTCTCTGGAAGACAACAG |
NGG |
2 |
0.4688 |
Tier II |
| 4 |
NC_000007.14 |
7 |
103871019 |
+ |
RELN |
NNNTCTCAGGCAGAAAACAG |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000002.12 |
2 |
99680436 |
+ |
AFF3 |
NNNTCCCGGGCAGACAGCAG |
NGG |
2 |
0.1604 |
Tier II |
| 6 |
NC_000017.11 |
17 |
42690728 |
- |
CNTNAP1 |
NNNTCTCTGGCAGACAAGAG |
NGG |
2 |
0.0833 |
Tier II |
| 7 |
NC_000016.10 |
16 |
67553473 |
- |
LOC124903703 |
NNNTCTCAGGCAGACAGCAG |
NGG |
2 |
0.1765 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
23300040 |
+ |
St6gal1 |
NNNTCTCAAGCAGACAACAG |
NGG |
2 |
0.6429 |
Tier II |
| 2 |
NC_000081.6 |
15 |
93292228 |
+ |
Yaf2 |
NNNTCTAGGGAAGACAACAG |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000083.6 |
17 |
29283307 |
- |
BC004004 |
NNNTGTCTGGCAGACAACAG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000083.6 |
17 |
29283307 |
- |
LOC115488789 |
NNNTGTCTGGCAGACAACAG |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000080.6 |
14 |
29153543 |
+ |
Cacna2d3 |
NNNTCTGGGGCAGGCAACAG |
NGG |
2 |
0.2196 |
Tier II |
| 6 |
NC_000082.6 |
16 |
4140450 |
- |
Crebbp |
NNNTCTAGGGCAGACAACAG |
NAG |
2 |
0.1944 |
Tier II |
| 7 |
NC_000069.6 |
3 |
79124683 |
- |
Rapgef2 |
NNNTCTCGGGAAGAGAACAG |
NGG |
2 |
0.0375 |
Tier II |
| 8 |
NC_000071.6 |
5 |
30440473 |
+ |
Otof |
NNNTCTCGGGCAGACCAGAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000070.6 |
4 |
101390188 |
+ |
0610043K17Rik |
NNNTCTGAGGCAGACAACAG |
NGG |
2 |
0.4706 |
Tier III |
Other clones with same target sequence:
(none)
