Construct: sgRNA BRDN0001147701
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAAACCATCGTAAGCTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CB (5291)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76191
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
138714659 |
- |
PIK3CB |
NNNAACCATCGTAAGCTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
108112213 |
- |
LEF1 |
NNNAACCATCATAAGCTCAT |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000023.11 |
X |
64193654 |
+ |
AMER1 |
NNNGACCATCCTAAGCTCAG |
NGG |
2 |
0.2679 |
Tier II |
| 4 |
NC_000002.12 |
2 |
189800747 |
+ |
PMS1 |
NNNAACCATCCTAGGCTCAG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000002.12 |
2 |
222252046 |
- |
PAX3 |
NNNAACCATCCTTAGCTCAG |
NGG |
2 |
0.1286 |
Tier II |
| 6 |
NC_000002.12 |
2 |
1104731 |
- |
SNTG2 |
NNNAACCACCGTAAGCTGAG |
NGG |
2 |
0.1231 |
Tier II |
| 7 |
NC_000012.12 |
12 |
5314016 |
+ |
LOC105369617 |
NNNAGCCATCTTAAGCTCAG |
NGG |
2 |
0.2769 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
99073770 |
- |
Pik3cb |
NNNAACCGTCGTAAGCTCAG |
NGA |
2 |
0.0509 |
Tier I |
| 2 |
NC_000080.6 |
14 |
23667091 |
+ |
Kcnma1 |
NNNAACCAGAGTAAGCTCAG |
NGG |
2 |
0.5365 |
Tier II |
| 3 |
NC_000069.6 |
3 |
116697438 |
+ |
Slc35a3 |
NNNAACCATCATAAGCACAG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000067.6 |
1 |
131272698 |
+ |
Ikbke |
NNNAACCATTGTAAGCTCTG |
NGG |
2 |
0.5068 |
Tier II |
| 5 |
NC_000084.6 |
18 |
75454417 |
- |
Ctif |
NNNAACCATAGTAAGTTCAG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000075.6 |
9 |
70347553 |
+ |
Myo1e |
NNNGACCATCGTAAGTTCAG |
NGG |
2 |
0.1923 |
Tier II |
| 7 |
NC_000082.6 |
16 |
58470830 |
+ |
St3gal6 |
NNNACCCATCGTAAGCTCAG |
NAG |
2 |
0.1296 |
Tier II |
| 8 |
NC_000070.6 |
4 |
131903191 |
- |
Gm12992 |
NNNAACCGTCGTAAGCTCAG |
NGA |
2 |
0.0509 |
Tier III |
| 9 |
NC_000070.6 |
4 |
131903191 |
- |
Gm12978 |
NNNAACCGTCGTAAGCTCAG |
NGA |
2 |
0.0509 |
Tier III |
Other clones with same target sequence:
(none)
