Construct: sgRNA BRDN0001147702
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATGGACAGTCATTAACCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGI3 (260425)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76550
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
113642110 |
+ |
MAGI3 |
NNNGGACAGTCATTAACCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
124351676 |
- |
SH2D1A |
NNNTGACAGTCACTAACCAA |
NGG |
2 |
0.2871 |
Tier II |
| 3 |
NC_000018.10 |
18 |
50189465 |
- |
MYO5B |
NNNGAACAGTCCTTAACCAA |
NGG |
2 |
0.2281 |
Tier II |
| 4 |
NC_000011.10 |
11 |
129155648 |
+ |
ARHGAP32 |
NNNGGAAAGTCATTTACCAA |
NGG |
2 |
0.15 |
Tier II |
| 5 |
NC_000002.12 |
2 |
75566091 |
+ |
EVA1A |
NNNGGATAGTCATTAACCAA |
NTG |
2 |
0.0317 |
Tier II |
| 6 |
NC_000001.11 |
1 |
148845000 |
- |
PDE4DIP |
NNNGGAGAGTCATTAAGCAA |
NGG |
2 |
0.0277 |
Tier II |
| 7 |
NC_000001.11 |
1 |
149285249 |
+ |
LOC124904395 |
NNNGGAGAGTCATTAAGCAA |
NGG |
2 |
0.0277 |
Tier II |
| 8 |
NC_000001.11 |
1 |
120233553 |
- |
LOC100996723 |
NNNGGAGAGTCATTAAGCAA |
NGG |
2 |
0.0277 |
Tier III |
| 9 |
NC_000001.11 |
1 |
120664858 |
+ |
PDE4DIPP2 |
NNNGGAGAGTCATTAAGCAA |
NGG |
2 |
0.0277 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
81307834 |
+ |
Asic2 |
NNNGGGCAGCCATTAACCAA |
NGG |
2 |
0.381 |
Tier II |
| 2 |
NC_000077.6 |
11 |
3839534 |
- |
Osbp2 |
NNNGGACAGACATTAACCAA |
NAG |
2 |
0.2222 |
Tier II |
| 3 |
NC_000069.6 |
3 |
84137115 |
- |
Mnd1 |
NNNTGACAGTCATTAACCAT |
NGG |
2 |
0.2182 |
Tier II |
| 4 |
NC_000073.6 |
7 |
81552719 |
+ |
Fsd2 |
NNNGGACAGTCAACAACCAA |
NGG |
2 |
0.1978 |
Tier II |
| 5 |
NC_000075.6 |
9 |
107675112 |
+ |
Gnat1 |
NNNGGACAGTCACTAACCAC |
NGG |
2 |
0.1794 |
Tier II |
| 6 |
NC_000077.6 |
11 |
67545175 |
- |
Gas7 |
NNNGGACAGACATTTACCAA |
NGG |
2 |
0.1714 |
Tier II |
| 7 |
NC_000081.6 |
15 |
20802178 |
+ |
Cdh12 |
NNNGGACATTCATTCACCAA |
NGG |
2 |
0.1455 |
Tier II |
| 8 |
NC_000076.6 |
10 |
61692881 |
- |
Sar1a |
NNNGGACAGTCTTTAGCCAA |
NGG |
2 |
0.0641 |
Tier II |
| 9 |
NC_000078.6 |
12 |
44562059 |
+ |
Nrcam |
NNNGGTCAGTCATTAAGCAA |
NGG |
2 |
0.042 |
Tier II |
| 10 |
NC_000075.6 |
9 |
29913376 |
- |
Ntm |
NNNGGACAGTCAAGAACCAA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000078.6 |
12 |
69844255 |
+ |
Map4k5 |
NNNGGACAGTCATGCACCAA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000079.6 |
13 |
92888163 |
+ |
Gm31630 |
NNNGGACAGTCATTTACCAT |
NGG |
2 |
0.12 |
Tier III |
| 13 |
NC_000074.6 |
8 |
109113971 |
- |
D030068K23Rik |
NNNGGACAGTCATGAACCAT |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000078.6 |
12 |
69844255 |
+ |
4931403G20Rik |
NNNGGACAGTCATGCACCAA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
