Construct: sgRNA BRDN0001147703
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTATGTGGAGTAATCTTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAN3 (255967)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77425
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28256334 |
- |
PAN3 |
NNNATGTGGAGTAATCTTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
51223458 |
+ |
FAM124A |
NNNATCTGGAGTAATCCTGG |
NGG |
2 |
0.4813 |
Tier II |
| 3 |
NC_000003.12 |
3 |
121715615 |
- |
GOLGB1 |
NNNATGTGGTGGAATCTTGG |
NGG |
2 |
0.4412 |
Tier II |
| 4 |
NC_000017.11 |
17 |
17047740 |
+ |
MPRIP |
NNNATTTGGAGTAAACTTGG |
NGG |
2 |
0.386 |
Tier II |
| 5 |
NC_000003.12 |
3 |
183267405 |
+ |
MCF2L2 |
NNNATGTGGAGTGATCATGG |
NGG |
2 |
0.3478 |
Tier II |
| 6 |
NC_000003.12 |
3 |
183267405 |
+ |
B3GNT5 |
NNNATGTGGAGTGATCATGG |
NGG |
2 |
0.3478 |
Tier II |
| 7 |
NC_000006.12 |
6 |
139279610 |
+ |
TXLNB |
NNNATGTGAAGTAATCATGG |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000004.12 |
4 |
167110233 |
- |
SPOCK3 |
NNNATGTGGAATACTCTTGG |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000001.11 |
1 |
13594013 |
+ |
PDPN |
NNNATGTTGAGTAATCTTGG |
NTG |
2 |
0.0244 |
Tier II |
| 10 |
NC_000002.12 |
2 |
226877779 |
+ |
RHBDD1 |
NNNATGTGGACTAATCTTGG |
NGT |
2 |
0.0069 |
Tier II |
| 11 |
NC_000007.14 |
7 |
40981909 |
- |
SUGCT |
NNNATGTGGAGTAATTTTGG |
NGC |
2 |
0.0068 |
Tier II |
| 12 |
NC_000006.12 |
6 |
139279610 |
+ |
LOC102723690 |
NNNATGTGAAGTAATCATGG |
NGG |
2 |
0.3429 |
Tier III |
| 13 |
NC_000001.11 |
1 |
59144483 |
- |
HSD52 |
NNNATGTTGATTAATCTTGG |
NGG |
2 |
0.2404 |
Tier III |
| 14 |
NC_000004.12 |
4 |
129953051 |
- |
LINC02465 |
NNNATGTGAAGTAATCTTGG |
NAG |
2 |
0.1667 |
Tier III |
| 15 |
NC_000014.9 |
14 |
57531972 |
- |
LOC105370519 |
NNNATGTGGAGTTATGTTGG |
NGG |
2 |
0.0462 |
Tier III |
| 16 |
NC_000006.12 |
6 |
30045570 |
- |
POLR1HASP |
NNNATGTGGAGTAGTCTTGG |
NGA |
2 |
0.0324 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
147519547 |
- |
Pan3 |
NNNGTGTGGAGTAATTTTGG |
NGG |
2 |
0.1923 |
Tier I |
| 2 |
NC_000084.6 |
18 |
31834446 |
+ |
Wdr33 |
NNNATGTGTAGTAAACTTGG |
NGG |
2 |
0.3088 |
Tier II |
| 3 |
NC_000073.6 |
7 |
140108773 |
- |
Echs1 |
NNNATGTAGAGTCATCTTGG |
NGG |
2 |
0.2105 |
Tier II |
| 4 |
NC_000078.6 |
12 |
3821798 |
+ |
Dnmt3a |
NNNATGTGGATGAATCTTGG |
NGG |
2 |
0.1923 |
Tier II |
| 5 |
NC_000076.6 |
10 |
90041027 |
- |
Anks1b |
NNNATGTGGAGTGATCGTGG |
NGG |
2 |
0.0767 |
Tier II |
| 6 |
NC_000082.6 |
16 |
67046270 |
+ |
Cadm2 |
NNNATGTGGAGTCATTTTGG |
NGG |
2 |
0.0648 |
Tier II |
Other clones with same target sequence:
(none)
