Construct: sgRNA BRDN0001147705

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGGTTCACCCCACAGTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IP6K3 (117283)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77656

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 33735283 - IP6K3 NNNTTCACCCCACAGTACAA NGG 0 1.0 Tier I
2 NC_000003.12 3 48695095 - IP6K2 NNNTTCACTCCCCAGTACAA NGG 2 0.2303 Tier I
3 NC_000005.10 5 128531797 + FBN2 NNNTTCACCAAACAGTACAA NGG 2 0.65 Tier II
4 NC_000018.10 18 11704110 + GNAL NNNTTCAACCCACAATACAA NGG 2 0.6118 Tier II
5 NC_000009.12 9 138080145 + CACNA1B NNNTTCACTCCTCAGTACAA NGG 2 0.2917 Tier II
6 NC_000009.12 9 8839943 + PTPRD NNNTTCACCATACAGTACAA NGG 2 0.2667 Tier II
7 NC_000017.11 17 44942222 + KIF18B NNNTTCAGCCCAAAGTACAA NGG 2 0.2473 Tier II
8 NC_000001.11 1 202616026 + SYT2 NNNATCCCCCCACAGTACAA NGG 2 0.2083 Tier II
9 NC_000016.10 16 12206709 + SNX29 NNNTTCACCCCACAGAGCAA NGG 2 0.1604 Tier II
10 NC_000021.9 21 34596794 - RCAN1 NNNTTCACTCCACAGTGCAA NGG 2 0.1544 Tier II
11 NC_000005.10 5 19503384 - CDH18 NNNTTGACCCCACAGTACCA NGG 2 0.1034 Tier II
12 NC_000006.12 6 15266948 - JARID2 NNNTGCACCCCACATTACAA NGG 2 0.0914 Tier II
13 NC_000020.11 20 51508179 - NFATC2 NNNTTCAGCCCACAGTAGAA NGG 2 0.0857 Tier II
14 NC_000002.12 2 53799515 + ERLEC1 NNNTTTACCCCACAGTACAA NGC 2 0.0206 Tier II
15 NC_000002.12 2 53799515 + GPR75-ASB3 NNNTTTACCCCACAGTACAA NGC 2 0.0206 Tier II
16 NC_000023.11 X 65373133 + ZC3H12B NNNTTCACCCCACAGTACGA NTG 2 0.0146 Tier II
17 NC_000011.10 11 19779789 - NAV2 NNNTTCACCCAACAGTACAA NGT 2 0.0121 Tier II
18 NC_000023.11 X 88497534 - LOC107985713 NNNTTCATCCCACAGTAAAA NGG 2 0.4712 Tier III
19 NC_000002.12 2 190757032 + LOC124907946 NNNTTTACCCCACAGTACAA NGA 2 0.0645 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 108796392 + Ip6k2 NNNTTCACTCCCCAGTACAA NGG 2 0.2303 Tier I
2 NC_000075.6 9 108024446 + Ip6k1 NNNTTCACCCCAGAGTACAA NGG 1 0.1364 Tier I
3 NC_000070.6 4 8544283 - Rab2a NNNTTCATCCCACAGAACAA NGG 2 0.7955 Tier II
4 NC_000067.6 1 66303561 - Map2 NNNTGCACCCCACAGTACAA NGG 1 0.64 Tier II
5 NC_000079.6 13 115078951 - Itga1 NNNCTCACCTCACAGTACAA NGG 2 0.609 Tier II
6 NC_000072.6 6 37011166 - Dgki NNNTTCAGCTCACAGTACAA NGG 2 0.605 Tier II
7 NC_000074.6 8 32888574 + Nrg1 NNNTTGACTCCACAGTACAA NGG 2 0.4375 Tier II
8 NC_000074.6 8 32888574 + Gm3985 NNNTTGACTCCACAGTACAA NGG 2 0.4375 Tier II
9 NC_000073.6 7 107415036 + Syt9 NNNTTCACACCACAGGACAA NGG 2 0.2967 Tier II
10 NC_000077.6 11 102921968 + Kif18b NNNTTCAGCCCAAAGTACAA NGG 2 0.2473 Tier II
11 NC_000074.6 8 13371748 - Tfdp1 NNNGTCACCCCACAGTACCA NGG 2 0.1293 Tier II
12 NC_000069.6 3 67349590 + Rsrc1 NNNTGCACCCCAGAGTACAA NGG 2 0.0873 Tier II
13 NC_000073.6 7 132286218 + Chst15 NNNTTCACCCCACAGTGCAA NAG 2 0.0458 Tier II
14 NC_000080.6 14 12399444 - Cadps NNNTTCACCCCACAATACAA NGT 2 0.0152 Tier II
15 NC_000074.6 8 46529148 - Acsl1 NNNTTCACCCCACAGCACAA NGC 2 0.0148 Tier II
16 NC_000074.6 8 32888574 + Gm51527 NNNTTGACTCCACAGTACAA NGG 2 0.4375 Tier III
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Other clones with same target sequence:

(none)