Construct: sgRNA BRDN0001147705
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGTTCACCCCACAGTACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K3 (117283)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77656
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000006.12 |
6 |
33735283 |
- |
IP6K3 |
NNNTTCACCCCACAGTACAA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000003.12 |
3 |
48695095 |
- |
IP6K2 |
NNNTTCACTCCCCAGTACAA |
NGG |
2 |
0.2303 |
Tier I |
3 |
NC_000005.10 |
5 |
128531797 |
+ |
FBN2 |
NNNTTCACCAAACAGTACAA |
NGG |
2 |
0.65 |
Tier II |
4 |
NC_000018.10 |
18 |
11704110 |
+ |
GNAL |
NNNTTCAACCCACAATACAA |
NGG |
2 |
0.6118 |
Tier II |
5 |
NC_000009.12 |
9 |
138080145 |
+ |
CACNA1B |
NNNTTCACTCCTCAGTACAA |
NGG |
2 |
0.2917 |
Tier II |
6 |
NC_000009.12 |
9 |
8839943 |
+ |
PTPRD |
NNNTTCACCATACAGTACAA |
NGG |
2 |
0.2667 |
Tier II |
7 |
NC_000017.11 |
17 |
44942222 |
+ |
KIF18B |
NNNTTCAGCCCAAAGTACAA |
NGG |
2 |
0.2473 |
Tier II |
8 |
NC_000001.11 |
1 |
202616026 |
+ |
SYT2 |
NNNATCCCCCCACAGTACAA |
NGG |
2 |
0.2083 |
Tier II |
9 |
NC_000016.10 |
16 |
12206709 |
+ |
SNX29 |
NNNTTCACCCCACAGAGCAA |
NGG |
2 |
0.1604 |
Tier II |
10 |
NC_000021.9 |
21 |
34596794 |
- |
RCAN1 |
NNNTTCACTCCACAGTGCAA |
NGG |
2 |
0.1544 |
Tier II |
11 |
NC_000005.10 |
5 |
19503384 |
- |
CDH18 |
NNNTTGACCCCACAGTACCA |
NGG |
2 |
0.1034 |
Tier II |
12 |
NC_000006.12 |
6 |
15266948 |
- |
JARID2 |
NNNTGCACCCCACATTACAA |
NGG |
2 |
0.0914 |
Tier II |
13 |
NC_000020.11 |
20 |
51508179 |
- |
NFATC2 |
NNNTTCAGCCCACAGTAGAA |
NGG |
2 |
0.0857 |
Tier II |
14 |
NC_000002.12 |
2 |
53799515 |
+ |
ERLEC1 |
NNNTTTACCCCACAGTACAA |
NGC |
2 |
0.0206 |
Tier II |
15 |
NC_000002.12 |
2 |
53799515 |
+ |
GPR75-ASB3 |
NNNTTTACCCCACAGTACAA |
NGC |
2 |
0.0206 |
Tier II |
16 |
NC_000023.11 |
X |
65373133 |
+ |
ZC3H12B |
NNNTTCACCCCACAGTACGA |
NTG |
2 |
0.0146 |
Tier II |
17 |
NC_000011.10 |
11 |
19779789 |
- |
NAV2 |
NNNTTCACCCAACAGTACAA |
NGT |
2 |
0.0121 |
Tier II |
18 |
NC_000023.11 |
X |
88497534 |
- |
LOC107985713 |
NNNTTCATCCCACAGTAAAA |
NGG |
2 |
0.4712 |
Tier III |
19 |
NC_000002.12 |
2 |
190757032 |
+ |
LOC124907946 |
NNNTTTACCCCACAGTACAA |
NGA |
2 |
0.0645 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000075.6 |
9 |
108796392 |
+ |
Ip6k2 |
NNNTTCACTCCCCAGTACAA |
NGG |
2 |
0.2303 |
Tier I |
2 |
NC_000075.6 |
9 |
108024446 |
+ |
Ip6k1 |
NNNTTCACCCCAGAGTACAA |
NGG |
1 |
0.1364 |
Tier I |
3 |
NC_000070.6 |
4 |
8544283 |
- |
Rab2a |
NNNTTCATCCCACAGAACAA |
NGG |
2 |
0.7955 |
Tier II |
4 |
NC_000067.6 |
1 |
66303561 |
- |
Map2 |
NNNTGCACCCCACAGTACAA |
NGG |
1 |
0.64 |
Tier II |
5 |
NC_000079.6 |
13 |
115078951 |
- |
Itga1 |
NNNCTCACCTCACAGTACAA |
NGG |
2 |
0.609 |
Tier II |
6 |
NC_000072.6 |
6 |
37011166 |
- |
Dgki |
NNNTTCAGCTCACAGTACAA |
NGG |
2 |
0.605 |
Tier II |
7 |
NC_000074.6 |
8 |
32888574 |
+ |
Nrg1 |
NNNTTGACTCCACAGTACAA |
NGG |
2 |
0.4375 |
Tier II |
8 |
NC_000074.6 |
8 |
32888574 |
+ |
Gm3985 |
NNNTTGACTCCACAGTACAA |
NGG |
2 |
0.4375 |
Tier II |
9 |
NC_000073.6 |
7 |
107415036 |
+ |
Syt9 |
NNNTTCACACCACAGGACAA |
NGG |
2 |
0.2967 |
Tier II |
10 |
NC_000077.6 |
11 |
102921968 |
+ |
Kif18b |
NNNTTCAGCCCAAAGTACAA |
NGG |
2 |
0.2473 |
Tier II |
11 |
NC_000074.6 |
8 |
13371748 |
- |
Tfdp1 |
NNNGTCACCCCACAGTACCA |
NGG |
2 |
0.1293 |
Tier II |
12 |
NC_000069.6 |
3 |
67349590 |
+ |
Rsrc1 |
NNNTGCACCCCAGAGTACAA |
NGG |
2 |
0.0873 |
Tier II |
13 |
NC_000073.6 |
7 |
132286218 |
+ |
Chst15 |
NNNTTCACCCCACAGTGCAA |
NAG |
2 |
0.0458 |
Tier II |
14 |
NC_000080.6 |
14 |
12399444 |
- |
Cadps |
NNNTTCACCCCACAATACAA |
NGT |
2 |
0.0152 |
Tier II |
15 |
NC_000074.6 |
8 |
46529148 |
- |
Acsl1 |
NNNTTCACCCCACAGCACAA |
NGC |
2 |
0.0148 |
Tier II |
16 |
NC_000074.6 |
8 |
32888574 |
+ |
Gm51527 |
NNNTTGACTCCACAGTACAA |
NGG |
2 |
0.4375 |
Tier III |
Other clones with same target sequence:
(none)