Construct: sgRNA BRDN0001147706
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTGCGGAAGAGCTGACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1E (1454)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77978
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
38298862 |
+ |
CSNK1E |
NNNTGCGGAAGAGCTGACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
38298862 |
+ |
TPTEP2-CSNK1E |
NNNTGCGGAAGAGCTGACGT |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000013.11 |
13 |
93847912 |
+ |
GPC6 |
NNNTGCTGAAGAGCTGACTT |
NGG |
2 |
0.381 |
Tier II |
| 4 |
NC_000017.11 |
17 |
263511 |
- |
RPH3AL |
NNNTTCGGAAGAGCTAACGT |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000001.11 |
1 |
182844457 |
- |
DHX9 |
NNNTGGGGAAGAGCTGAAGT |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000022.11 |
22 |
46494275 |
- |
CELSR1 |
NNNTGAGGAAGAGCTGAGGT |
NGG |
2 |
0.1238 |
Tier II |
| 7 |
NC_000016.10 |
16 |
87466072 |
+ |
ZCCHC14 |
NNNTGCAGAAGAGCTGACGC |
NGG |
2 |
0.0909 |
Tier II |
| 8 |
NC_000001.11 |
1 |
43995439 |
- |
CCDC24 |
NNNTGCGGAAGAGCTGAGTT |
NGG |
2 |
0.0889 |
Tier II |
| 9 |
NC_000006.12 |
6 |
39310273 |
- |
KCNK17 |
NNNTGCGGAAGAGCTGAGGT |
NGA |
2 |
0.0093 |
Tier II |
| 10 |
NC_000013.11 |
13 |
93847912 |
+ |
GPC6-AS2 |
NNNTGCTGAAGAGCTGACTT |
NGG |
2 |
0.381 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
131077128 |
- |
Mapkapk2 |
NNNTGGGGAAGAGCTGACAT |
NGG |
2 |
0.3571 |
Tier II |
| 2 |
NC_000074.6 |
8 |
111721669 |
+ |
Bcar1 |
NNNGGCGGAGGAGCTGACGT |
NGG |
2 |
0.3472 |
Tier II |
| 3 |
NC_000068.7 |
2 |
84363277 |
- |
Calcrl |
NNNTGCGGTAGAGCTGAAGT |
NGG |
2 |
0.3231 |
Tier II |
| 4 |
NC_000070.6 |
4 |
136736314 |
- |
Ephb2 |
NNNTGCTGAAGAGCTGAAGT |
NGG |
2 |
0.3077 |
Tier II |
| 5 |
NC_000079.6 |
13 |
54312647 |
- |
Cplx2 |
NNNTGCGGGAGAGCTGAGGT |
NGG |
2 |
0.0889 |
Tier II |
| 6 |
NC_000067.6 |
1 |
172195584 |
+ |
Dcaf8 |
NNNTGCTGAAGAGGTGACGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000070.6 |
4 |
43399244 |
+ |
Rusc2 |
NNNTGGGGAAGAGGTGACGT |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000067.6 |
1 |
90364694 |
+ |
Gm34992 |
NNNTGAGGAAGAGATGACGT |
NGG |
2 |
0.325 |
Tier III |
| 9 |
NC_000079.6 |
13 |
54312647 |
- |
LOC115488208 |
NNNTGCGGGAGAGCTGAGGT |
NGG |
2 |
0.0889 |
Tier III |
| 10 |
NC_000068.7 |
2 |
21912383 |
- |
Gm39767 |
NNNTGCGGAAGAGCTGAGGA |
NGG |
2 |
0.075 |
Tier III |
| 11 |
NC_000086.7 |
X |
74918784 |
+ |
Xlr3e-ps |
NNNTGCTGAAGAGCTTACGT |
NGG |
2 |
0.0 |
Tier III |
| 12 |
NC_000070.6 |
4 |
43399244 |
+ |
Gm52698 |
NNNTGGGGAAGAGGTGACGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
