Construct: sgRNA BRDN0001147708
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTATCCTGTCGAAGTAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80215
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
5840015 |
- |
ANO2 |
NNNATCCTGCCGAAGTAAAG |
NGG |
1 |
0.5333 |
Tier II |
| 2 |
NC_000007.14 |
7 |
157924159 |
- |
PTPRN2 |
NNNATCCTGTCCAAGTGAAG |
NGG |
2 |
0.0934 |
Tier II |
| 3 |
NC_000001.11 |
1 |
107298319 |
- |
NTNG1 |
NNNCTCCTGTCGAAGTAAAG |
NGA |
2 |
0.0245 |
Tier II |
| 4 |
NC_000002.12 |
2 |
225518321 |
+ |
NYAP2 |
NNNATCCTGTAGAAGTAAAG |
NGT |
2 |
0.0121 |
Tier II |
| 5 |
NC_000009.12 |
9 |
104656302 |
- |
LOC107987105 |
NNNATCATGTAGAAGTAAAG |
NGG |
2 |
0.5625 |
Tier III |
| 6 |
NC_000001.11 |
1 |
192845489 |
+ |
LOC105371667 |
NNNATGATGTCGAAGTAAAG |
NGG |
2 |
0.375 |
Tier III |
| 7 |
NC_000001.11 |
1 |
192845489 |
+ |
LOC105371668 |
NNNATGATGTCGAAGTAAAG |
NGG |
2 |
0.375 |
Tier III |
| 8 |
NC_000008.11 |
8 |
123757482 |
+ |
LOC105375739 |
NNNATCCTGTAGAAGTAAAG |
NGT |
2 |
0.0121 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
37726592 |
+ |
Zfp536 |
NNNATCCTGTGGAGGTAAAG |
NGG |
2 |
0.1167 |
Tier II |
| 2 |
NC_000077.6 |
11 |
68134424 |
- |
Stx8 |
NNNATCCTGTGGAAGTAAAG |
NAG |
2 |
0.0648 |
Tier II |
| 3 |
NC_000070.6 |
4 |
116325605 |
+ |
Mast2 |
NNNATCCTGTCGAAGGGAAG |
NGG |
2 |
0.0611 |
Tier II |
| 4 |
NC_000070.6 |
4 |
137175501 |
- |
Gm46872 |
NNNATCCTGTCAAAGTAGAG |
NGG |
2 |
0.3733 |
Tier III |
Other clones with same target sequence:
(none)
